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AB326732

GFP ELISA Kit, Chemiluminescent - Intracellular

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GFP ELISA Kit, Chemiluminescent - Intracellular is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of GFP with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 0.82 - 1800 pg/ml with a sensitivity of 0.26 pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending
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Sandwich ELISA - GFP ELISA Kit, Chemiluminescent - Intracellular (AB326732)
  • sELISA

Lab

Sandwich ELISA - GFP ELISA Kit, Chemiluminescent - Intracellular (AB326732)

Dynamic range comparison between the Chemiluminescent GFP SimpleStep ELISA and colorimetric GFP SimpleStep ELISA (ab316916). The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Sandwich ELISA - GFP ELISA Kit, Chemiluminescent - Intracellular (AB326732)
  • sELISA

Lab

Sandwich ELISA - GFP ELISA Kit, Chemiluminescent - Intracellular (AB326732)

Standard curve comparison between the Chemiluminescent GFP SimpleStep ELISA and colorimetric GFP SimpleStep ELISA (ab316916) in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - GFP ELISA Kit, Chemiluminescent - Intracellular (AB326732)
  • sELISA

Supplier Data

Sandwich ELISA - GFP ELISA Kit, Chemiluminescent - Intracellular (AB326732)

Interpolated concentrations of spiked eGFP in Jurkat extract and spiked denatured-eGFP in Jurkat extract

Recombinant untreated eGFP and 2% SDS denatured-eGFP were diluted 1 : 20 in 500 µg/mL Jurkat extract (prepared in 1X Cell extraction Buffer PTR Without Enhancer) and then diluted in a 2-fold dilution series in 1X Cell Extraction Buffer PTR. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - GFP ELISA Kit, Chemiluminescent - Intracellular (AB326732)
  • sELISA

Supplier Data

Sandwich ELISA - GFP ELISA Kit, Chemiluminescent - Intracellular (AB326732)

Interpolated concentrations of GFP in HEK293 overexpressing GFP cell extract

Interpolated concentration of native GFP was measured in duplicate at different sample concentrations. Undiluted samples are 50 ng/mL HEK293 overexpressing GFP cell extract.  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

주요 정보

검출 방식

Luminescent

샘플 타입

Tissue Extracts, Cell Lysate

Reacts with

Aequorea victoria

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 0.26 pg/mL

검출 범위

0.82 - 1800 pg/mL

분석 소요 시간

55m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

GFP ELISA Kit, Chemiluminescent - Intracellular (ab326732) is a rapid single-wash sandwich 55-min ELISA to measure GFP in Tissue Extracts, Cell Lysate with a sensitivity of 0.26 pg/mL.

How the assay works

GFP ELISA Kit, Chemiluminescent - Intracellular (ab326732) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

GFP (ab326732) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-GFP antibody [EPR28018-88] - BSA and Aside free (Capture) ab315128
- Anti-GFP antibody [EPR28018-89] - BSA and Aside free (Detector) ab315129

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6.1" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6.9" } ]
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Recovery

[ { "sample": "Tissue Extracts", "range": "82 - 107 %", "average": "= 107" }, { "sample": "Cell Lysate", "range": "82 - 92 %", "average": "= 92" } ]
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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab326732-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB326732-CMP11", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent B", "number":"AB326732-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB326732-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"GFP Lyophilized Recombinant Protein", "number":"AB326732-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB326732-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X GFP Capture Antibody", "number":"AB326732-CMP01", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent A", "number":"AB326732-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X GFP Detector Antibody", "number":"AB326732-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB326732-CMP05", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB326732-CMP04", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB326732-CMP06", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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제품 프로토콜

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타겟 정보

Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
See full target information GFP

대체 명칭 보기

Green fluorescent protein, GFP
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websiteProtocolBooklet
en
websiteProtocolBooklet
en
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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