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AB316916

GFP ELISA Kit- Intracellular

GFP ELISA 키트- Intracellular

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GFP ELISA Kit- Intracellular is a single-wash 90-min Simplestep used to quantify GFP with a sensitivity of 2.523 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

See more GFP assay kits and antibodies
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Sandwich ELISA - GFP ELISA Kit- Intracellular (AB316916)
  • sELISA

Supplier Data

Sandwich ELISA - GFP ELISA Kit- Intracellular (AB316916)

Interpolated concentrations of spiked eGFP in Jurkat extract and spiked denatured-eGFP in Jurkat extract

Recombinant untreated eGFP and 2% SDS denatured-eGFP were diluted 1 : 20 in 500 µg/mL Jurkat extract (prepared in 1X Cell extraction Buffer PTR Without Enhancer) and then diluted in a 2-fold dilution series in 1X Cell Extraction Buffer PTR. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - GFP ELISA Kit- Intracellular (AB316916)
  • sELISA

Supplier Data

Sandwich ELISA - GFP ELISA Kit- Intracellular (AB316916)

Interpolated concentrations of GFP in HEK293 overexpressing GFP cell extract

Interpolated concentration of native GFP was measured in duplicate at different sample concentrations. Undiluted samples are 50 ng/mL HEK293 overexpressing GFP cell extract.  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - GFP ELISA Kit- Intracellular (AB316916)
  • sELISA

Supplier Data

Sandwich ELISA - GFP ELISA Kit- Intracellular (AB316916)

Example of GFP standard curve in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - GFP ELISA Kit- Intracellular (AB316916)
  • sELISA

Supplier Data

Sandwich ELISA - GFP ELISA Kit- Intracellular (AB316916)

Standard curve comparison between the original GFP SimpleStep ELISA (ab171581) and current GFP SimpleStep ELISA (ab316916).

주요 정보

검출 방식

Colorimetric

샘플 타입

Tissue Extracts, Cell Lysate

Reacts with

Aequorea victoria

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 2.523 pg/mL

검출 범위

9.375 - 600 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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보완 제품

Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

  • 5
  • 24
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Secondary Antibodies

AB205718

Goat Anti-Rabbit IgG H&L (HRP)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (AB205718)

  • 5
  • 8
Assay Kits

AB221829

mCherry ELISA Kit

Recombinant|SimpleStep

ELISA - mCherry ELISA Kit (AB221829)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

GFP SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of GFP protein in Tissue extracts, Cell Lysate. Quantitate  GFP with 2.523 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.1" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.9" } ]
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Recovery

[ { "sample": "Tissue Extracts", "range": "82 - 107 %", "average": "= 93" }, { "sample": "Cell Lysate", "range": "82 - 92 %", "average": "= 86" } ]
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제품 구성

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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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제품 프로토콜

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타겟 정보

Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
See full target information GFP

대체 명칭 보기

Green fluorescent protein, GFP
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websiteProtocolBooklet
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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