Helicobacter pylori IgG plus ELISA Kit

제품 세부 정보

Helicobacter pylori IgG plus ELISA Kit ab178645 is a sandwich ELISA to measure Helicobacter pylori IgG plus in serum, citrate plasma, heparin plasma with a sensitivity of 1.39 IU/mL.

How the assay works:

The quantitative immunoenzymatic determination of specific antibodies is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique. Microplates are coated with specific antigens to bind corresponding antibodies of the sample. After washing the wells to remove all unbound sample material a horseradish peroxidase (HRP) labelled conjugate is added. This conjugate binds to the captured antibodies. In a second washing step unbound conjugate is removed. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of specific antibodies in the sample. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450/620 nm is read using an ELISA microwell plate reader.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Helicobacter pylori IgG plus ELISA Kit ab178645 protocol summary

1. Add 100 µL control, standard or sample to appropriate wells. Incubate for 1 hour at 37°
2. Aspirate and wash each well three times with 300 µL 1X Washing Solution
3. Add 100 µL of HRP conjugate to each well. Incubate for 30 minutes at room temperature. Repeat the washing steps.
4. Add 100 µL TMB Substrate Solution to each well and incubate for 30 minutes at room temperature.
5. Add 100 µL Stop Solution and read OD at 450/620 nm within 30 minutes after addition of the stop solution.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.