JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB205087

Horse SAA ELISA Kit

Be the first to review this product! 리뷰 제출

|

(0 출판물)

Horse SAA ELISA Kit is a Sandwich ELISA for the measurement of Horse SAA in Horse in Biofluids samples.
2 이미지
Sandwich ELISA - Horse SAA ELISA Kit (AB205087)
  • sELISA

Supplier Data

Sandwich ELISA - Horse SAA ELISA Kit (AB205087)

Representative standard curve using ab205087 Horse SAA ELISA Kit.

Sandwich ELISA - Horse SAA ELISA Kit (AB205087)
  • sELISA

Supplier Data

Sandwich ELISA - Horse SAA ELISA Kit (AB205087)

Representative standard curve using ab205087 Horse SAA ELISA Kit.

주요 정보

검출 방식

Colorimetric

샘플 타입

Citrate plasma, EDTA Plasma, Heparin Plasma, Serum

Reacts with

Horse

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 0.962 ng/mL

검출 범위

2.25 - 72 ng/mL

분석 소요 시간

1h 50m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

style
left-column

보완 제품

Assay Kits

AB222511

Mouse C Reactive Protein ELISA Kit

Recombinant|SimpleStep

ELISA - Mouse C Reactive Protein ELISA Kit (AB222511)

  • 0
  • 0
Assay Kits

AB178013

Human IL-6 ELISA Kit

KO Validated|Recombinant|SimpleStep

Sandwich ELISA - Human IL-6 ELISA Kit (AB178013)

  • 5
  • 2
Assay Kits

AB246537

Human SAP ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human SAP ELISA Kit (AB246537)

  • 0
  • 0
Assay Kits

AB185986

Human IL-10 ELISA Kit (Interleukin-10)

KO Validated|Recombinant|SimpleStep

ELISA - Human IL-10 ELISA Kit (Interleukin-10) (AB185986)

  • 0
  • 0
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

제품 세부 정보

SAA in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of SAA protein in horse biological fluids.

In this assay the SAA present in samples reacts with the anti-SAA antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, the Detection Antibody, biotin conjugated anti-SAA, is added and complexes are formed. Following a wash step, the horseradish peroxidase (HRP) conjugated Streptavidin is added and complexes are formed. After another washing step, the complexes are assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of SAA in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of SAA in the test sample. The quantity of SAA in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
style
left-column

Recovery

[ { "sample": "Serum", "range": null, "average": ">= 85" } ]
style
left-column

제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab205087-1x96Tests", "assetComponentDetails": [ { "size":"1 x 50 mL", "name":"5X Diluent Concentrate", "number":"AB205087-CMP09", "productcode":"" }, { "size":"1 x 50 mL", "name":"20X Wash Buffer Concentrate", "number":"AB205087-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Chromogen Substrate Solution", "number":"AB205087-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Horse SAA ELISA Microplate", "number":"AB205087-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB205087-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Horse SAA Calibrator (Lyophilized)", "number":"AB205087-CMP02", "productcode":"" }, { "size":"1 x 150 µL", "name":"100X Detection Antibody Conjugate", "number":"AB205087-CMP05", "productcode":"" }, { "size":"1 x 150 µL", "name":"100X HRP Streptavidin", "number":"AB205087-CMP06", "productcode":"" } ] } } }
style
left-column

특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
style
left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Serum Amyloid A (SAA) is an important acute-phase protein with a mass of approximately 12 kDa. It plays a role in inflammation and is present in the serum. SAA is an apolipoprotein that associates with high-density lipoprotein (HDL) during inflammatory states. The protein is expressed mainly in the liver and circulating SAA levels greatly increase during inflammatory responses marking it as an important marker for inflammation.
Biological function summary

SAA contributes to the body's immune response to injury by recruiting immune cells and facilitating the removal of cholesterol. As part of the acute-phase response SAA integrates into HDL displacing other apolipoproteins and altering HDL's function. Increased SAA levels can also modulate prostaglandin synthesis and secretion influencing the inflammatory response. The exact role of SAA in pathways beyond these functions continues to be a subject of research.

Pathways

SAA influences lipid metabolism and inflammatory pathways. It plays a significant role within the acute-phase response pathway which modulates the immune system’s reaction to stress such as infection or injury. Another pathway involves cholesterol metabolism where SAA interacts with apolipoproteins AI and AII altering the composition and function of HDL particles.

High levels of SAA associate with amyloidosis and chronic inflammatory diseases. In amyloidosis misfolded SAA proteins deposit as amyloid fibrils in tissues leading to organ dysfunction. In dogs and other species SAA is used as a biomarker to monitor inflammatory diseases. Its connection with C-reactive protein further emphasizes its role in inflammatory processes. Research continues to explore how modulating SAA levels may impact disease progression and treatment.
style
left-column

제품 프로토콜

style
left-column

타겟 정보

Major acute phase reactant. Apolipoprotein of the HDL complex.
See full target information SAA1

대체 명칭 보기

Serum amyloid A protein, SAA, SAA1
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com