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AB272033

Horse TIMP2 ELISA kit

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Horse TIMP2 ELISA kit is a Sandwich ELISA for the measurement of Horse TIMP2 in Horse, Human in Biofluids, Cell Culture Media samples.
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Sandwich ELISA - Horse TIMP2 ELISA kit (AB272033)
  • sELISA

Supplier Data

Sandwich ELISA - Horse TIMP2 ELISA kit (AB272033)

Horse TIMP-2 Standard Curve.

This standard curve is for demonstration only. A standard curve must be run with each assay.

Horse TIMP-2 ELISA kit (ab272033) Standard curve.

주요 정보

검출 방식

Colorimetric

샘플 타입

Plasma, Cell culture supernatant, Serum

Reacts with

Horse, Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 4 pg/mL

검출 범위

16.38 - 4000 pg/mL

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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보완 제품

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Horse TIMP-2 ELISA Kit is designed for the quantitative determination of Horse TIMP-2 in cell culture supernatants, plasma and serum samples.

This assay employs an antibody specific for TIMP-2 coated on a 96-well plate. Standards and samples are pipetted into the wells and TIMP-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Horse TIMP-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TIMP-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Detects horse and human TIMP2.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "131 - 149 %", "average": "= 141.3" }, { "sample": "Serum", "range": "135 - 148 %", "average": "= 141.2" }, { "sample": "Plasma", "range": "137 - 149 %", "average": "= 143.3" } ]
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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab272033-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB272033-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB272033-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Horse TIMP2 coated Microplate (12 x 8 wells)", "number":"AB272033-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB272033-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB272033-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"500X HRP-Streptavidin Concentrate", "number":"AB272033-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Horse TIMP2 Detection Antibody", "number":"AB272033-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Horse TIMP2 Standard (Lyophilized)", "number":"AB272033-CMP01", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
-20°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

TIMP2 also known as Tissue Inhibitor of Metalloproteinases 2 mechanistically inhibits the activity of matrix metalloproteinases (MMPs). It binds to MMPs regulating the extracellular matrix's remodeling. The protein has a molecular mass of around 21 kDa. TIMP2 is expressed in various tissues including the brain liver and kidneys where it maintains a balance between matrix degradation and production.
Biological function summary

TIMP2 plays a significant role in tissue maintenance and repair by controlling extracellular matrix turnover. It forms complexes with MMPs such as MMP-2 inhibiting their proteolytic activities. The protein is also involved in cell proliferation and differentiation affecting processes like angiogenesis and wound healing. Its regulatory function aids in maintaining tissue homeostasis and preventing excessive matrix breakdown.

Pathways

TIMP2 is integral to the matrix metalloproteinase pathway and the regulation of endothelial cell migration. The protein interacts closely with other MMPs influencing their activation and modulation. Through these pathways TIMP2 holds an important position in cellular migration and signaling ensuring precise matrix remodeling in response to physiological needs.

TIMP2 has significant links with cancer and fibrosis. Dysregulation of TIMP2 expression or its imbalance with MMPs can contribute to tumor progression and metastasis due to uncontrolled matrix degradation. Additionally abnormal TIMP2 levels associate with fibrotic diseases where excessive tissue deposition occurs. TIMP2's interaction with proteins like MMP-9 and MMP-2 highlights its involvement in these pathological conditions.
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제품 프로토콜

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타겟 정보

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them.
See full target information TIMP2

대체 명칭 보기

TIMP-2, TIMP2, Metalloproteinase inhibitor 2, Tissue inhibitor of metalloproteinases 2
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websiteProtocolBooklet
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Product promise

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