AB303748

Human ABP1 ELISA Kit

Recombinant
SimpleStep
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Human ABP1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human ABP1 with a sensitivity of 83.489 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

2 이미지

Sandwich ELISA - Human ABP1 ELISA Kit (AB303748)

sELISA

Supplier Data

Sandwich ELISA - Human ABP1 ELISA Kit (AB303748)

Interpolated concentrations of human ABP1 in THP-1 cell culture supernatant.

Interpolated concentration of native ABP1 was measured in duplicate at different sample concentrations. Undiluted samples are 50% THP-1 cell culture supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human ABP1 ELISA Kit (AB303748)

Example of human ABP1 standard curve in Sample Diluent NS.

Example of human ABP1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Serum, EDTA Plasma, Cell culture supernatant

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 83.489 pg/mL

검출 범위

187.5 - 12000 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Human ABP1 SimpleStep ELISA® kit (ab303748) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human ABP1 protein in serum, plasma (EDTA) and cell culture supernatant. Quantitate Human ABP1 with 83.489 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.24" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.11" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "91 - 106 %", "average": "= 96" }, { "sample": "EDTA Plasma", "range": "80 - 99 %", "average": "= 88" }, { "sample": "Serum", "range": "86 - 96 %", "average": "= 90" } ]

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제품 구성

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

+4°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

ABP1 also known as Auxin Binding Protein 1 is a protein with a molecular mass of approximately 22 kDa. This protein primarily functions as an auxin receptor in plants mediating various cellular responses to the plant hormone auxin. ABP1 locates mainly in the endoplasmic reticulum and is present across various plant tissues. The protein has a significant affinity for auxin and triggers specific intracellular signaling cascades essential for growth and development processes.

Biological function summary

ABP1 plays an important role in controlling cell elongation and division and it works in tandem with other auxin transporters. The protein functions within a broader receptor complex interacting with other proteins to modify plant cell physiology. Its action facilitates cell wall modification which directly influences the plant's response to environmental and endogenous signals. Through these interactions ABP1 significantly contributes to regulating the plant's phototropic and gravitropic responses.

Pathways

The operation of ABP1 integrates into the auxin signaling pathway and is associated with the regulation of gene expression. It links to TIR1 an important F-box protein in the SCF complex involved in the auxin signal transduction pathway. Together ABP1 and TIR1 coordinate to modulate the stability of transcription factors affecting downstream gene expression necessary for plant growth and development.

ABP1 itself mostly pertains to plant biology but its function offers insights into plant growth abnormalities. Disruptions in ABP1 function may result in disorders like auxin-insensitive phenotypes leading to defective root and shoot development. Additionally ABP1's interaction with PIN proteins responsible for auxin efflux creates potential for research into correcting growth defects in crops making it a critical focus in agricultural science.

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제품 프로토콜

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타겟 정보

Catalyzes the oxidative deamination of primary amines to the corresponding aldehydes with the concomitant production of hydrogen peroxide and ammonia (PubMed : 12072962, PubMed : 19764817, PubMed : 239684, PubMed : 8144586). Its preferred substrates are the diamines histamine and 1-methylhistamine and it could therefore play a role in allergic and immune responses (PubMed : 12072962). Has a broad specificity for diamines and can also act on cadaverine and putrescine, two products of amino acid catabolism (PubMed : 12072962). It could also act on polyamines, like spermidine and spermine though less efficiently, and regulate various biological processes (PubMed : 12072962, PubMed : 239684).

See full target information AOC1

대체 명칭 보기

ABP, ABP1, DAO, DAO1, AOC1, Diamine oxidase [copper-containing], Diamine oxidase, Amiloride-binding protein, Amiloride-binding protein 1, Amine oxidase copper domain-containing protein 1, Histaminase, Kidney amine oxidase, KAO, KDAO

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websiteProtocolBooklet

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Product promise

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Human ABP1 ELISA Kit

Sandwich ELISA - Human ABP1 ELISA Kit (AB303748)

Sandwich ELISA - Human ABP1 ELISA Kit (AB303748)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

결과 유형

Sensitivity

검출 범위

분석 소요 시간

분석 플랫폼

보완 제품

Assay Kits

Assay Kits

Assay Kits

Assay Kits

Reactivity 정보

제품 세부 정보

Precision

Recovery

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

Product promise