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AB324922

Human ACSL4/FACL4 ELISA Kit

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Human ACSL4/FACL4 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human ACSL4/FACL4 with a sensitivity of 86.5pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

-Colormetric sandwich ELISA - 450 nm readout- works on any standard plate reader.

-Different formats for different needs: 10 x 96 plates for bulk orders.

-Knock out validated for guaranteed specificity
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Sandwich ELISA - Human ACSL4/FACL4 ELISA Kit (AB324922)
  • sELISA

Lab

Sandwich ELISA - Human ACSL4/FACL4 ELISA Kit (AB324922)

Example of human ACSL4/FACL4 standard curve in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human ACSL4/FACL4 ELISA Kit (AB324922)
  • sELISA

Lab

Sandwich ELISA - Human ACSL4/FACL4 ELISA Kit (AB324922)

Interpolated concentration of native ACSL4/FACL4 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : HepG2 cell extract 2.5 µg/mL and liver tissue extract 500 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human ACSL4/FACL4 ELISA Kit (AB324922)
  • sELISA

Lab

Sandwich ELISA - Human ACSL4/FACL4 ELISA Kit (AB324922)

Interpolated concentration of native ACSL4/FACL4 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : 62.5 µg/mL wild type and ACSL4/FACL4 knockout A-431 cell extracts. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR. The mean concentration was determined to be 11,031 pg/mL in wild type extract and undetectable in knockout extract. The ACSL4/FACL4 A-431 knockout cell line is available as ab269613.

주요 정보

검출 방식

Colorimetric

샘플 타입

Cell Extract, Tissue Extracts

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 86.5 pg/mL

검출 범위

218.75 - 14000 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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보완 제품

Primary Antibodies

AB125066

Anti-Glutathione Peroxidase 4 antibody [EPNCIR144]

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (AB125066)

  • 5
  • 13
Primary Antibodies

AB155282

Anti-FACL4 antibody [EPR8640]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-FACL4 antibody [EPR8640] (AB155282)

  • 4
  • 1
Primary Antibodies

AB307601

Anti-xCT antibody [EPR27115-64]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-xCT antibody [EPR27115-64] (AB307601)

  • 5
  • 1
Assay Kits

AB285291

Mouse Estrogen ELISA Kit

Competitive ELISA - Mouse Estrogen ELISA Kit (AB285291)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Human ACSL4/FACL4 ELISA Kit (ab324922) is a rapid single-wash sandwich 90-min ELISA to measure Human ACSL4/FACL4 in Cell Extract, Tissue Extract. This SimpleStep sensitivity is 86.5 pg/ml.

How the assay works

Human ACSL4/FACL4 ELISA Kit (ab324922) SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human ACSL4/FACL4 (ab324922) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.9" } ]
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Recovery

[ { "sample": "Cell Lysate", "range": "103 - 117 %", "average": "= 108" }, { "sample": "Tissue Extracts", "range": "88 - 99 %", "average": "= 95" } ]
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제품 구성

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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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제품 프로토콜

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타겟 정보

Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoA for both synthesis of cellular lipids, and degradation via beta-oxidation (PubMed : 21242590, PubMed : 22633490, PubMed : 24269233). Preferentially activates arachidonate and eicosapentaenoate as substrates (PubMed : 21242590). Preferentially activates 8,9-EET > 14,15-EET > 5,6-EET > 11,12-EET. Modulates glucose-stimulated insulin secretion by regulating the levels of unesterified EETs (By similarity). Modulates prostaglandin E2 secretion (PubMed : 21242590).
See full target information ACSL4

대체 명칭 보기

ACS4, FACL4, LACS4, ACSL4, Long-chain-fatty-acid--CoA ligase 4, Arachidonate--CoA ligase, Long-chain acyl-CoA synthetase 4, LACS 4
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websiteProtocolBooklet
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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