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AB326009

Human AFP ELISA Kit, Chemiluminescent

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Human AFP ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human AFP with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 18.3 - 40000 pg/ml with a sensitivity of 13.9 pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending
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Sandwich ELISA - Human AFP ELISA Kit, Chemiluminescent (AB326009)
  • sELISA

Lab

Sandwich ELISA - Human AFP ELISA Kit, Chemiluminescent (AB326009)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human AFP ELISA Kit, Chemiluminescent (AB326009)
  • sELISA

Lab

Sandwich ELISA - Human AFP ELISA Kit, Chemiluminescent (AB326009)

Dynamic range comparison between the Chemiluminescent Human alpha Fetoprotein SimpleStep ELISA, colorimetric Human alpha Fetoprotein SimpleStep ELISA (ab193765), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Sandwich ELISA - Human AFP ELISA Kit, Chemiluminescent (AB326009)
  • sELISA

Lab

Sandwich ELISA - Human AFP ELISA Kit, Chemiluminescent (AB326009)

Serum from ten individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean AFP concentration was determined to be 678.4 pg/mL with a range of 133.9 – 2,257 pg/mL.

Sandwich ELISA - Human AFP ELISA Kit, Chemiluminescent (AB326009)
  • sELISA

Lab

Sandwich ELISA - Human AFP ELISA Kit, Chemiluminescent (AB326009)

Interpolated concentrations of native AFP in pregnant human serum and HepG2 cell culture supernatant samples. The concentrations of AFP were measured in duplicates, interpolated from the AFP standard curves and corrected for sample dilution. Undiluted samples are as follows : pregnant human serum 3% and HepG2 cell culture supernatant 1 : 666. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean AFP concentration was determined to be 221.8 ng/mL in neat pregnant human serum and 6,165 ng/mL in neat HepG2 cell culture supernatant.

Sandwich ELISA - Human AFP ELISA Kit, Chemiluminescent (AB326009)
  • sELISA

Lab

Sandwich ELISA - Human AFP ELISA Kit, Chemiluminescent (AB326009)

Serum from ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean AFP concentration in nine detectable donor samples was determined to be 550.7 pg/mL with a range of 247.9-816.1 pg/mL. Donor #5 was not detectable.

Sandwich ELISA - Human AFP ELISA Kit, Chemiluminescent (AB326009)
  • sELISA

Lab

Sandwich ELISA - Human AFP ELISA Kit, Chemiluminescent (AB326009)

Standard curve comparison between the Chemiluminescent Human alpha Fetoprotein SimpleStep ELISA and colorimetric Human alpha Fetoprotein SimpleStep ELISA (ab193765) in Sample Diluent 75BS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human AFP ELISA Kit, Chemiluminescent (AB326009)
  • sELISA

Lab

Sandwich ELISA - Human AFP ELISA Kit, Chemiluminescent (AB326009)

Interpolated concentration of native alpha Fetoprotein was measured in duplicate using the chemiluminescent version of this kit at different sample concentrations. Undiluted samples are as follows : serum 100% and plasma (citrate) 100%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent 75BS.

주요 정보

검출 방식

Luminescent

샘플 타입

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant, Cell culture media, Urine

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 13.9 pg/mL

검출 범위

18.3 - 40000 pg/mL

분석 소요 시간

55m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Human AFP ELISA Kit, Chemiluminescent (ab326009) is a rapid single-wash sandwich 55-min ELISA to measure Human AFP in Serum, Citrate Plasma, EDTA Plasma, Heparin Plasma, Cell Culture Supernatant, Cell Culture Media, Urine with a sensitivity of 13.9 pg/mL.

How the assay works

Human AFP ELISA Kit, Chemiluminescent (ab326009) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human AFP (ab326009) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-alpha 1 Fetoprotein antibody [EPR20365-101] - BSA and Azide free (Capture) ab252573
- Anti-alpha 1 Fetoprotein antibody [EPR20364-26] - BSA and Azide free (Detector) ab252632
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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.5" } ]
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Recovery

[ { "sample": "Serum", "range": "88 - 96 %", "average": "= 91" }, { "sample": "Citrate plasma", "range": "89 - 89 %", "average": "= 89" }, { "sample": "EDTA Plasma", "range": "98 - 102 %", "average": "= 100" }, { "sample": "Heparin Plasma", "range": "94 - 97 %", "average": "= 96" }, { "sample": "Cell culture supernatant", "range": "101 - 113 %", "average": "= 103" }, { "sample": "Cell culture media", "range": "91 - 98 %", "average": "= 94" }, { "sample": "Urine", "range": "116 - 121 %", "average": "= 119" } ]
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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab326009-1x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Vial", "name":"Human alpha Fetoprotein Lyophilized Recombinant Protein", "number":"AB326009-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Human alpha Fetoprotein Detector Antibody (Lyophilized)", "number":"AB326009-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Human alpha Fetoprotein Capture Antibody (Lyophilized)", "number":"AB326009-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB326009-CMP11", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB326009-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB326009-CMP10", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB326009-CMP04", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent B", "number":"AB326009-CMP09", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent A", "number":"AB326009-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB326009-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent 75BS", "number":"AB326009-CMP05", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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제품 프로토콜

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타겟 정보

Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
See full target information AFP

대체 명칭 보기

HPAFP, AFP, Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin
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websiteProtocolBooklet
en
websiteProtocolBooklet
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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