AB325255

Human ALK-1 (ACVRL1) ELISA Kit

SimpleStep
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Human ALK-1 (ACVRL1) ELISA Kit is a Sandwich ELISA for the measurement of Human ALK-1 (ACVRL1) in Human in Cell/Tissue Extracts samples.

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Sandwich ELISA - Human ALK-1 (ACVRL1) ELISA Kit (AB325255)

sELISA

Lab

Sandwich ELISA - Human ALK-1 (ACVRL1) ELISA Kit (AB325255)

Interpolated concentration of native ALK-1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : EA.hy926 cell extract 75 µg/mL and human lung tissue extract 125 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer SSW.

sELISA

Lab

Sandwich ELISA - Human ALK-1 (ACVRL1) ELISA Kit (AB325255)

Example of human ALK-1 standard curve in 96-well vs. 384-well plate in 1X Cell Extraction Buffer SSW. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Human ALK-1 (ACVRL1) ELISA Kit (AB325255)

Interpolated concentration of native ALK-1 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 37.5 µg/mL EA.hy926 cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer SSW.

sELISA

Lab

Sandwich ELISA - Human ALK-1 (ACVRL1) ELISA Kit (AB325255)

Example of human ALK-1 standard curve in 1X Cell Extraction Buffer SSW. Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Cell Lysate, Tissue Extracts

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 9.65 pg/mL

검출 범위

17.188 - 1100 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Human ALK-1 (ACVRL1) ELISA Kit (ab325255) is a rapid single-wash sandwich 90-min ELISA to measure Human ALK-1 (ACVRL1) in Cell Extract, Tissue Extract. This SimpleStep sensitivity is 9.65 pg/ml.

How the assay works

Human ALK-1 (ACVRL1) ELISA Kit (ab325255) SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human ALK-1 (ACVRL1) (ab325255) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Save your precious samples

Our Human ALK-1 (ACVRL1) ELISA Kit only requires 12.5µL sample.

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.5" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 7" } ]

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Recovery

[ { "sample": "Cell Lysate", "range": "107 - 108 %", "average": "= 108" }, { "sample": "Tissue Extracts", "range": "100 - 108 %", "average": "= 105" } ]

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제품 구성

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

+4°C

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제품 프로토콜

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타겟 정보

Type I receptor for TGF-beta family ligands BMP9/GDF2 and BMP10 and important regulator of normal blood vessel development. On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. May bind activin as well.

See full target information ACVRL1

대체 명칭 보기

ACVRLK1, ALK1, ACVRL1, Activin receptor type-1-like, Activin receptor-like kinase 1, Serine/threonine-protein kinase receptor R3, TGF-B superfamily receptor type I, ALK-1, SKR3, TSR-I

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websiteProtocolBooklet

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human ALK-1 (ACVRL1) ELISA Kit

Sandwich ELISA - Human ALK-1 (ACVRL1) ELISA Kit (AB325255)

Sandwich ELISA - Human ALK-1 (ACVRL1) ELISA Kit (AB325255)

Sandwich ELISA - Human ALK-1 (ACVRL1) ELISA Kit (AB325255)

Sandwich ELISA - Human ALK-1 (ACVRL1) ELISA Kit (AB325255)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

결과 유형

Sensitivity

검출 범위

분석 소요 시간

분석 플랫폼

보완 제품

Assay Kits

Assay Kits

Assay Kits

Primary Antibodies

Reactivity 정보

제품 세부 정보

Precision

Recovery

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

제품 프로토콜

타겟 정보

대체 명칭 보기

Product promise