AB108798

Human alpha 1 Antitrypsin ELISA Kit (SERPINA1)

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(4 제품이 사용된 논문 )

Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) is a Competitive ELISA for the measurement of Human alpha 1 Antitrypsin (SERPINA1) in Human in Biofluids samples.

대체 명칭 보기

AAT, PI, PRO0684, PRO2209, SERPINA1, Alpha-1-antitrypsin, Alpha-1 protease inhibitor, Alpha-1-antiproteinase, Serpin A1

2 이미지

Competitive ELISA - Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (AB108798)

cELISA

Unknown

Competitive ELISA - Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (AB108798)

Alpha 1 Antitrypsin measured in biological fluids showing quantity (μg) per mL of tested sample. Serum and plasma samples diluted 100-8100 fold. Human milk and saliva diluted 10 fold. Hu urine diluted 1000 fold.

cELISA

Supplier Data

Competitive ELISA - Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (AB108798)

Standard curve : mean of duplicates (+/- SD) with background reads subtracted

주요 정보

검출 방식

Colorimetric

샘플 타입

Plasma, Serum

Reacts with

Human

분석 유형

Competitive

Sensitivity

= 0.13 µg/mL

검출 범위

0.156 - 160 µg/mL

분석 소요 시간

분석 플랫폼

Microplate

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

alpha 1 Antitrypsin (SERPINA1) Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab108798) is designed for the quantitative measurement of alpha 1 antitrypsin levels in plasma and serum.

An alpha 1 antitrypsin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated alpha 1 Antitrypsin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of alpha 1 antitrypsin captured in plate.

Get results in 90 minutes with Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (ab189579) from our SimpleStep ELISA^® range.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.1" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.2" } ]

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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108798-1x96Tests", "assetComponentDetails": [ { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB108798-CMP09", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent N Concentrate", "number":"AB108798-CMP02", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB108798-CMP08", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB108798-CMP03", "productcode":"" }, { "size":"1 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB108798-CMP06", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB108798-CMP07", "productcode":"" }, { "size":"1 x 1 Vial", "name":"1X Biotinylated Human alpha 1 Antitrypsin (Lyophilized)", "number":"AB108798-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"alpha 1 Antitrypsin Standard", "number":"AB108798-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"alpha 1 Antitrypsin Microplate (12 x 8 well strips)", "number":"AB108798-CMP04", "productcode":"" } ] } } }

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

-20°C

적절한 장기 보관 조건

Multi

보관 정보

Please refer to protocols

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Alpha 1 Antitrypsin also known as A1AT or alpha-1 proteinase inhibitor is a serine protease inhibitor with a molecular mass of about 52 kDa. This protein mainly expresses in the liver and found in high concentrations in the blood plasma. A1AT protects tissues from enzymes of inflammatory cells especially neutrophil elastase. Its expression level is regulated by the liver making it a significant player in maintaining tissue integrity.

Biological function summary

A1AT regulates protease activity by forming complexes with target enzymes. It specifically inhibits neutrophil elastase a powerful enzyme capable of degrading elastin an important component of connective tissues. A1AT prevents excessive tissue damage during inflammation by maintaining a balance in protease activity within connective tissues across various organs.

Pathways

Alpha 1 Antitrypsin functions within the proteolytic pathways involved in inflammatory response and tissue remodeling. A1AT is closely related to neutrophil elastase in these processes. It interacts with other protease inhibitors like alpha 2-macroglobulin reinforcing its protective role against enzymatic activity that can lead to tissue destruction under pathophysiological conditions.

Alpha 1 Antitrypsin deficiency is a genetic condition that can cause chronic obstructive pulmonary disease (COPD) and liver cirrhosis. Deficient A1AT levels result in unregulated elastase activity leading to lung tissue damage and impaired liver function. Other proteins such as MMP-9 collaborate with elastase in exacerbating tissue damage illustrating how insufficient A1AT can significantly contribute to disease development.

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제품 프로토콜

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타겟 정보

Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.. Short peptide from AAT. Reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).

See full target information SERPINA1

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제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

JCI insight 5: PubMed32379724

2020

Development of an RNAi therapeutic for alpha-1-antitrypsin liver disease.

Applications

Unspecified application

Species

Unspecified reactive species

Christine I Wooddell,Keith Blomenkamp,Ryan M Peterson,Vladimir M Subbotin,Christian Schwabe,James Hamilton,Qili Chu,Dawn R Christianson,Julia O Hegge,John Kolbe,Holly L Hamilton,Maria F Branca-Afrazi,Bruce D Given,David L Lewis,Edward Gane,Steven B Kanner,Jeffrey H Teckman

Scientific reports 10:8290 PubMed32427833

2020

Aggregation of M3 (E376D) variant of alpha1- antitrypsin.

Applications

Unspecified application

Species

Unspecified reactive species

Arif Bashir,Younis Hazari,Debnath Pal,Dibyajyoti Maity,Samirul Bashir,Laishram Rajendrakumar Singh,Naveed Nazir Shah,Khalid Majid Fazili

Journal of the American College of Nutrition 40:98-103 PubMed32275481

2020

Reduced 25(OH) Vitamin D Association with Lower Alpha-1-Antitrypsin Blood Levels in Type 2 Diabetic Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Virginia M Lindley,Kamal Bhusal,Laura Huning,Steven N Levine,Sushil K Jain

PeerJ 4:e2450 PubMed27672505

2016

Tissue and serum samples of patients with papillary thyroid cancer with and without benign background demonstrate different altered expression of proteins.

Applications

cELISA

Species

Human

Mardiaty Iryani Abdullah,Ching Chin Lee,Sarni Mat Junit,Khoon Leong Ng,Onn Haji Hashim

제품이 사용된 논문 모두 보기

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websiteProtocolBooklet

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human alpha 1 Antitrypsin ELISA Kit (SERPINA1)

Competitive ELISA - Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (AB108798)

Competitive ELISA - Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (AB108798)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

Sensitivity

검출 범위

분석 소요 시간

분석 플랫폼

보완 제품

Assay Kits

Assay Kits

Assay Kits

Assay Kits

Reactivity 정보

제품 세부 정보

Precision

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (4)

Product promise