JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB326752

Human AMH ELISA Kit, Chemiluminescent

Be the first to review this product! 리뷰 제출

|

(0 출판물)

Human AMH ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human AMH with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 3.89 - 8500 pg/ml with a sensitivity of 2.85 pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending
6 이미지
Sandwich ELISA - Human AMH ELISA Kit, Chemiluminescent (AB326752)
  • sELISA

Lab

Sandwich ELISA - Human AMH ELISA Kit, Chemiluminescent (AB326752)

Standard curve comparison between the Chemiluminescent Human AMH SimpleStep ELISA and colorimetric Human AMH SimpleStep ELISA (ab318930) in Sample Diluent NS + 1X Enhancer. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human AMH ELISA Kit, Chemiluminescent (AB326752)
  • sELISA

Lab

Sandwich ELISA - Human AMH ELISA Kit, Chemiluminescent (AB326752)

Dynamic range comparison between the Chemiluminescent Human AMH SimpleStep ELISA, colorimetric Human AMH SimpleStep ELISA (ab318930), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Sandwich ELISA - Human AMH ELISA Kit, Chemiluminescent (AB326752)
  • sELISA

Supplier Data

Sandwich ELISA - Human AMH ELISA Kit, Chemiluminescent (AB326752)

Interpolated concentrations of human AMH in serum, plasma (citrate), plasma (EDTA), and plasma (heparin). Interpolated concentration of native AMH was measured in duplicate at different sample concentrations. Undiluted samples are 45% serum, plasma (citrate), plasma (EDTA), and plasma (heparin).  The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS + 1X Enhancer.

Sandwich ELISA - Human AMH ELISA Kit, Chemiluminescent (AB326752)
  • sELISA

Supplier Data

Sandwich ELISA - Human AMH ELISA Kit, Chemiluminescent (AB326752)

Interpolated concentrations of AMH in normal human serum donors. Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean AMH concentration of detected samples was determined to be 2,090 pg/mL with a range of undetected – 6,208 pg/mL.

Sandwich ELISA - Human AMH ELISA Kit, Chemiluminescent (AB326752)
  • sELISA

Supplier Data

Sandwich ELISA - Human AMH ELISA Kit, Chemiluminescent (AB326752)

Interpolated concentrations of AMH in normal human serum donors. Serum of ten individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean AMH concentration was determined to be 2,698 pg/mL with a range of 998 – 4,674 pg/mL.

Sandwich ELISA - Human AMH ELISA Kit, Chemiluminescent (AB326752)
  • sELISA

Supplier Data

Sandwich ELISA - Human AMH ELISA Kit, Chemiluminescent (AB326752)

Interpolated concentrations of human AMH in monkey serum and rat serum. Interpolated concentration of native AMH was measured in duplicate at different sample concentrations. Undiluted samples are 22.5% rat serum, and 2.25% monkey serum.  The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS + 1X Enhancer.

주요 정보

검출 방식

Luminescent

샘플 타입

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media

Reacts with

Human, Rat, Monkey

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 2.85 pg/mL

검출 범위

3.89 - 8500 pg/mL

분석 소요 시간

55m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

제품 세부 정보

Human AMH ELISA Kit, Chemiluminescent (ab326752) is a rapid single-wash sandwich 55-min ELISA to measure Human AMH in Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media with a sensitivity of 2.85 pg/mL.

How the assay works

Human AMH ELISA Kit, Chemiluminescent (ab326752) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human AMH (ab326752) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-AMH antibody [EPR25835-80] - BSA and Aside free (Capture) ab314564
- Anti-AMH antibody [EPR25767-77] - BSA and Aside free (Detector) ab314565
style
left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6.1" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 10.6" } ]
style
left-column

Recovery

[ { "sample": "Serum", "range": "87 - 108 %", "average": "= 108" }, { "sample": "Citrate plasma", "range": "100 - 117 %", "average": "= 117" }, { "sample": "EDTA Plasma", "range": "94 - 105 %", "average": "= 105" }, { "sample": "Heparin Plasma", "range": "86 - 105 %", "average": "= 105" }, { "sample": "Cell culture media", "range": "82 - 106 %", "average": "= 106" } ]
style
left-column

제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab326752-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB326752-CMP11", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB326752-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB326752-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human AMH Capture Antibody", "number":"AB326752-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human AMH Lyophilized Recombinant Protein", "number":"AB326752-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB326752-CMP10", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent B", "number":"AB326752-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB326752-CMP06", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB326752-CMP05", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent A", "number":"AB326752-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human AMH Detector Antibody", "number":"AB326752-CMP02", "productcode":"" } ] } } }
style
left-column

특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
style
left-column
style
left-column

제품 프로토콜

style
left-column

타겟 정보

The anti-Muellerian hormone (AMH) plays an important role in several reproductive functions (PubMed : 14742691, PubMed : 34155118, PubMed : 3754790, PubMed : 8469238). Anti-Muellerian hormone binds and activates AMHR2, its specific type-II receptor, that heterodimerizes with type-I receptors (ACVR1 and BMPR1A) to regulate target gene expression through downstream SMAD protein signal transduction (PubMed : 20861221, PubMed : 34155118). Produced and secreted by Sertoli cells of the male fetus, anti-Muellerian hormone induces Muellerian duct regression during male fetal sexual differentiation (PubMed : 34155118, PubMed : 3754790, PubMed : 8469238). In female, it is produced by granulosa cells of the preantral and small antral follicles and acts as a negative regulator of the primordial to primary follicle transition and decreases FSH sensitivity of growing follicles (PubMed : 14742691). Also plays a role in Leydig cell differentiation and function (By similarity).
See full target information AMH

대체 명칭 보기

MIF, AMH, Anti-Muellerian hormone, Muellerian-inhibiting factor, Muellerian-inhibiting substance, MIS
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com