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AB99970

Human Angiogenin ELISA Kit (ANG)

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(7 제품이 사용된 논문 )

Human Angiogenin ELISA Kit (ANG) is a Sandwich ELISA for the measurement of Human Angiogenin (ANG) in Human in Biofluids, Cell Culture Media samples.
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Sandwich ELISA - Human Angiogenin ELISA Kit (ANG) (AB99970)
  • sELISA

Supplier Data

Sandwich ELISA - Human Angiogenin ELISA Kit (ANG) (AB99970)

Typical Standard Curve.

Representative standard curve using ab99970.

Sandwich ELISA - Human Angiogenin ELISA Kit (ANG) (AB99970)
  • sELISA

Supplier Data

Sandwich ELISA - Human Angiogenin ELISA Kit (ANG) (AB99970)

Representative standard curve using ab99970.

주요 정보

검출 방식

Colorimetric

샘플 타입

Plasma, Cell culture supernatant, Serum

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

< 1.5 pg/mL

검출 범위

1.64 - 400 pg/mL

분석 플랫폼

Microplate

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Abcam's Angiogenin (ANG) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Angiogenin in serum, plasma and cell culture supernates.

This assay employs an antibody specific for Human Angiogenin coated on a 96-well plate. Standards and samples are pipetted into the wells and Angiogenin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Angiogenin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Angiogenin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Optimisation may be required with urine samples.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "85 - 104 %", "average": "= 95.37" }, { "sample": "Serum", "range": "82 - 105 %", "average": "= 95.95" }, { "sample": "Plasma", "range": "83 - 104 %", "average": "= 93.49" } ]
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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab99970-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB99970-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB99970-CMP07", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB99970-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Angiogenin Microplate (12 x 8 well strip)", "number":"AB99970-CMP08", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB99970-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB99970-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human Angiogenin Standard (lyophilized)", "number":"AB99970-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human Angiogenin", "number":"AB99970-CMP09", "productcode":"" }, { "size":"1 x 200 µL", "name":"700X HRP-Streptavidin Concentrate", "number":"AB99970-CMP02", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
-20°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Angiogenin also known as ANG is a small protein with a molecular mass of approximately 14 kDa. It belongs to the ribonuclease A superfamily. Angiogenin expression occurs in a variety of tissues with notably pronounced levels in the liver pancreas and placenta indicating its widespread biological importance. In addition to its primary name it appears in the context of research under alternative names like KIT ANG and elisa ang reflecting its use and identification in various experimental assays.
Biological function summary

Angiogenin significantly contributes to the process of angiogenesis the formation of new blood vessels from pre-existing vascular structures. It characterizes acts as both a ribonuclease and a potent angiogenic factor. Although not part of a multi-protein complex angiogenin interacts with endothelial cells to promote their growth and migration. Angiogenin facilitates these cellular behaviors by triggering pathways that enhance cell survival and proliferation essential for healthy tissue development and repair.

Pathways

Angiogenin is intricately involved in the angiogenesis pathway a critical process for both physiological and pathological conditions. It interacts with proteins such as VEGF (vascular endothelial growth factor) which plays an essential role in vascular function. Angiogenin's interaction with these pathways enhances cellular responses to external stimuli maintaining vascular integrity and supporting tissue regeneration under stress or injury conditions.

Angiogenin has connections to conditions like cancer and amyotrophic lateral sclerosis (ALS). Aberrant regulation of angiogenin can contribute to tumorigenesis by promoting excessive angiogenesis aiding the growth and spread of cancerous cells. In ALS altered angiogenin function associates with neurodegenerative processes linking its function to proteins such as superoxide dismutase 1 (SOD1). Understanding angiogenin's role in these diseases aids in uncovering therapeutic targets and developing treatment strategies.
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제품 프로토콜

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타겟 정보

Secreted ribonuclease that can either promote or restrict cell proliferation of target cells, depending on the context (PubMed : 12051708, PubMed : 1400510, PubMed : 19332886, PubMed : 20129916, PubMed : 21855800, PubMed : 23047679, PubMed : 23843625, PubMed : 2424496, PubMed : 2459697, PubMed : 2730651, PubMed : 27518564, PubMed : 28176817, PubMed : 29100074, PubMed : 29748193, PubMed : 3122207, PubMed : 32510170, PubMed : 38718836, PubMed : 8159680, PubMed : 8570639, PubMed : 8622921, PubMed : 9578571). Endocytosed in target cells via its receptor PLXNB2 and translocates to the cytoplasm or nucleus (PubMed : 29100074, PubMed : 32510170). Under stress conditions, localizes to the cytoplasm and promotes the assembly of stress granules (SGs) : specifically cleaves a subset of tRNAs within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs), resulting in translation repression and inhibition of cell proliferation (PubMed : 1400510, PubMed : 19332886, PubMed : 20129916, PubMed : 21855800, PubMed : 23047679, PubMed : 27518564, PubMed : 29100074, PubMed : 29748193, PubMed : 32510170, PubMed : 38718836). tiRNas also prevent formation of apoptosome, thereby promoting cell survival (By similarity). Preferentially cleaves RNAs between a pyrimidine and an adenosine residue, suggesting that it cleaves the anticodon loop of tRNA(Ala) (32-UUAGCAU-38) after positions 33 and 36 (PubMed : 3289612, PubMed : 38718836). Cleaves a subset of tRNAs, including tRNA(Ala), tRNA(Glu), tRNA(Gly), tRNA(Lys), tRNA(Val), tRNA(His), tRNA(Asp) and tRNA(Sec) (PubMed : 31582561). Under growth conditions and in differentiated cells, translocates to the nucleus and stimulates ribosomal RNA (rRNA) transcription, including that containing the initiation site sequences of 45S rRNA, thereby promoting cell growth and proliferation (PubMed : 12051708, PubMed : 15735021, PubMed : 27518564, PubMed : 29100074, PubMed : 8127865). Angiogenin induces vascularization of normal and malignant tissues via its ability to promote rRNA transcription (PubMed : 19354288, PubMed : 4074709, PubMed : 8448182). Involved in hematopoietic stem and progenitor cell (HSPC) growth and survival by promoting rRNA transcription in growth conditions and inhibiting translation in response to stress, respectively (PubMed : 27518564). Mediates the crosstalk between myeloid and intestinal epithelial cells to protect the intestinal epithelial barrier integrity : secreted by myeloid cells and promotes intestinal epithelial cells proliferation and survival (PubMed : 32510170). Also mediates osteoclast-endothelial cell crosstalk in growing bone : produced by osteoclasts and protects the neighboring vascular cells against senescence by promoting rRNA transcription (By similarity).
See full target information ANG

대체 명칭 보기

RNASE5, ANG, Angiogenin, Ribonuclease 5, RNase 5
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제품이 사용된 논문 (7)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of oncology 2020:3531652 PubMed33204262

2020

Prevents the Recurrences of Resected Colonic Polyps by Affecting Angiogenin-2-Induced Protein Kinase B/Akt Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Qiang Gao,Guihong Yu,Minghui Yu,Xinping Wang

Translational cancer research 8:2316-2327 PubMed35116984

2019

Identification of molecular markers for pre-thrombotic state: validation in the rabbits with tibia fracture or lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Qi,Xin Hu,Jing Chen,Qian Wu,Xiaobin Ying,Yan Shi

BBA clinical 8:28-34 PubMed28725572

2017

Novel panel of protein biomarkers to predict response to bortezomib-containing induction regimens in multiple myeloma patients.

Applications

Unspecified application

Species

Unspecified reactive species

Kay Reen Ting,Michael Henry,Justine Meiller,Annemarie Larkin,Martin Clynes,Paula Meleady,Despina Bazou,Paul Dowling,Peter O'Gorman

Oncotarget 7:43109-43123 PubMed27317771

2016

Angiogenin contributes to bladder cancer tumorigenesis by DNMT3b-mediated MMP2 activation.

Applications

Unspecified application

Species

Unspecified reactive species

Rafael Peres,Hideki Furuya,Ian Pagano,Yoshiko Shimizu,Kanani Hokutan,Charles J Rosser

Materials (Basel, Switzerland) 8:4299-4315 PubMed28793441

2015

Poly(Dopamine)-Assisted Immobilization of Xu Duan on 3D Printed Poly(Lactic Acid) Scaffolds to Up-Regulate Osteogenic and Angiogenic Markers of Bone Marrow Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Chia-Hung Yeh,Yi-Wen Chen,Ming-You Shie,Hsin-Yuan Fang

Oncogene 34:890-901 PubMed24561529

2014

Angiogenin promotes tumoral growth and angiogenesis by regulating matrix metallopeptidase-2 expression via the ERK1/2 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

M Miyake,S Goodison,A Lawton,E Gomes-Giacoia,C J Rosser

Molecular cancer research : MCR 12:322-34 PubMed24464915

2014

PAI-1 leads to G1-phase cell-cycle progression through cyclin D3/cdk4/6 upregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Evan Gomes Giacoia,Makito Miyake,Adrienne Lawton,Steve Goodison,Charles J Rosser
제품이 사용된 논문 모두 보기
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Product promise

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