Human Apo-H ELISA Kit (Apolipoprotein H)
- Recombinant
- SimpleStep
- 각 태그의 뜻
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- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- sELISA
Supplier Data
Sandwich ELISA - Human Apo-H ELISA Kit (Apolipoprotein H) (AB274403)
Interpolated concentrations of native Apo-H in human serum and plasma samples.
The concentrations of Apo-H were measured in duplicates, interpolated from the Apo-H standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 140,000, plasma (Heparin) 1 : 80,000, plasma (EDTA) 1 : 80,000 and plasma (Citrate) 1 : 80,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Apo-H concentration was determined to be 164.87 μg/ml in serum, 91.63 μg/ml in plasma (Heparin) and 82.17 μg/ml in plasma (EDTA) and 82.25 μg/ml in plasma (Citrate).
- sELISA
Supplier Data
Sandwich ELISA - Human Apo-H ELISA Kit (Apolipoprotein H) (AB274403)
Example of human Apo-H standard curve in Sample Diluent NS.
The Apo-H standard curve was prepared as described in Section 10.1. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Human Apo-H ELISA Kit (Apolipoprotein H) (AB274403)
Interpolated concentrations of native Apo-H in human HepG2 Cell extract based on a 200 μg/mL extract load.
The concentrations of Apo-H were measured in duplicate and interpolated from the Apo-H standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Apo-H concentration was determined to be 1307.93 pg/mL.
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제품 세부 정보
Human Apo-H ELISA kit (ab274403) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Apo-H protein in serum, plasma and lystates. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Apo-H with 10.4 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Apo-H, also known as beta-2 glycoprotein 1, is a key serum protein involved in agglutination response in the blood. It contains a highly positively charged stretch of amino acids which bind negatively charged agglutinizing factors. Improper expression of apo-H has been noted in many vascular disease states such as, cardiovascular disease, diabetes, and thrombosis. The immunogen used to raise the antibodies for this kit was the full-length mature protein. This protein does not share high sequence identity in mouse or rat samples (76.1 and 82.2%, respectively) and, therefore, is not expected to cross-react.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Apo-H acts as an anticoagulant and participates in the modulation of immune response. It forms complexes with lipid-bound proteins facilitating the clearance of these complexes from the bloodstream. This interaction is key in processes like platelet aggregation and stabilization of membranes. Apo-H also influences the removal of oxidized proteins and lipids reducing cellular stress and preventing tissue damage.
Pathways
Apo-H plays a critical role in the coagulation cascade and lipid metabolism. It interacts with proteins such as prothrombin and fibrinogen influencing blood coagulation pathways. Apo-H's involvement in the clearance of apoptotic cells integrates into the lipid metabolic pathway highlighting its relationship with apolipoproteins and other lipid-related proteins. These interactions illustrate the multi-functional aspects of Apo-H within these pathways.
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Recent publications for all applications. Explore the 전체 목록 and refine your search
Cancer diagnosis & prognosis 4:735-742 PubMed39502607
2024
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