Human Apolipoprotein CI/Apo-CI ELISA Kit- Extracellular
- Recombinant
- SimpleStep
- 각 태그의 뜻
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- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- sELISA
Supplier Data
Sandwich ELISA - Human Apolipoprotein CI/Apo-CI ELISA Kit- Extracellular (AB320791)
Interpolated concentrations of Apolipoprotein CI/Apo-CI in normal human serum donors. Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean Apolipoprotein CI/Apo-CI concentration was determined to be 179.9 µg/mL with a range of 98.2 – 282.7 µg/mL.
- sELISA
Supplier Data
Sandwich ELISA - Human Apolipoprotein CI/Apo-CI ELISA Kit- Extracellular (AB320791)
Interpolated concentrations of human Apolipoprotein CI/Apo-CI in HepG2 cell culture supernatant. Interpolated concentration of native Apolipoprotein CI/Apo-CI was measured in duplicate at different sample concentrations. Undiluted samples are 6% HepG2 cell culture supernatant. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
- sELISA
Supplier Data
Sandwich ELISA - Human Apolipoprotein CI/Apo-CI ELISA Kit- Extracellular (AB320791)
Interpolated concentrations of human Apolipoprotein CI/Apo-CI in serum in 96-well vs. 384-well plates. Interpolated concentration of native Apolipoprotein CI/Apo-CI was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1 : 100,000 serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
- sELISA
Supplier Data
Sandwich ELISA - Human Apolipoprotein CI/Apo-CI ELISA Kit- Extracellular (AB320791)
Interpolated concentrations of human Apolipoprotein CI/Apo-CI in serum, plasma – citrate, plasma – EDTA, and plasma – heparin. Interpolated concentration of native Apolipoprotein CI/Apo-CI was measured in duplicate at different sample concentrations. Undiluted samples are serum 1 : 100,000, plasma – citrate 1 : 100,000, plasma – EDTA 1 : 100,000, and plasma – heparin 1 : 80,000. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
- sELISA
Supplier Data
Sandwich ELISA - Human Apolipoprotein CI/Apo-CI ELISA Kit- Extracellular (AB320791)
Example of human Apolipoprotein CI/Apo-CI standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Example of human Apolipoprotein CI/Apo-CI standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Human Apolipoprotein CI/Apo-CI ELISA Kit- Extracellular (AB320791)
Example of human Apolipoprotein CI/Apo-CI standard curve in Sample Diluent NS. Example of human Apolipoprotein CI/Apo-CI standard curve. Background-subtracted data values (mean +/- SD) are graphed.
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Reactivity 정보
제품 세부 정보
Human Apolipoprotein CI/Apo-CI SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Apolipoprotein CI/Apo-CI protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture media. Quantitate Human Apolipoprotein CI/Apo-CI with 14.44 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.
Precision
Recovery
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특성 및 보관 정보
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적절한 단기 보관 조건
적절한 장기 보관 조건
보관 정보
추가 정보
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Apo-CI influences plasma triglyceride levels and cholesterol homeostasis. It plays a role in lipid transport and metabolism by modulating the binding of other apolipoproteins such as apo A-IV and interfering with the binding of lipoproteins to their receptors. Apo-CI is part of a complex of apolipoproteins that regulate lipid metabolism working in tandem to balance lipid and cholesterol levels in the body.
Pathways
Apo-CI is actively involved in the lipoprotein metabolism pathway and cholesterol efflux pathway. These pathways are important in maintaining lipid balance and cardiovascular health. Apo-CI is related to other apolipoproteins within these pathways such as apo E and apo A-I. Its function in modulating lipoprotein receptor interactions aligns with the broader mechanisms of these pathways in lipid and cholesterol regulation.
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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