AB119505

Human APRIL ELISA Kit

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Human APRIL ELISA Kit is a Sandwich ELISA for the measurement of Human APRIL in Human in Biofluids, Cell Culture Media samples.

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Sandwich ELISA - Human APRIL ELISA Kit (AB119505)

sELISA

Supplier Data

Sandwich ELISA - Human APRIL ELISA Kit (AB119505)

Representative Standard Curve using ab119505.

주요 정보

검출 방식

Colorimetric

샘플 타입

Plasma, Cell culture supernatant, Serum

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 0.4 ng/mL

검출 범위

0.78 - 50 ng/mL

분석 플랫폼

Microplate

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Abcam's APRIL Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human APRIL concentrations in cell culture supernatant, serum and plasma (citrate, EDTA, heparin).

APRIL specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and along with an APRIL biotinylated detection antibody and the microplate is then incubated at room temperature. Following washing with wash buffer a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and then unbound conjugates are washed away using wash buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of APRIL captured on the plate.

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Precision & recovery 정보

Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.1" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.1" } ]

Recovery

[ { "sample": "Serum", "range": null, "average": "= 76" } ]

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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab119505-1x96Tests", "assetComponentDetails": [ { "size":"1 x 5 mL", "name":"20X Assay Buffer Concentrate", "number":"AB119505-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"TMB Substrate Solution", "number":"AB119505-CMP09", "productcode":"" }, { "size":"4 x 1 Unit", "name":"Adhesive Films", "number":"AB119505-CMP01", "productcode":"" }, { "size":"1 x 15 mL", "name":"Stop Solution (1M Phosphoric acid)", "number":"AB119505-CMP07", "productcode":"" }, { "size":"1 x 50 mL", "name":"20X Wash Buffer Concentrate", "number":"AB119505-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Microplate coated with polyclonal antibody to Human APRIL (12 x 8 wells)", "number":"AB119505-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent", "number":"AB119505-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human APRIL Standard", "number":"AB119505-CMP05", "productcode":"" }, { "size":"1 x 70 µL", "name":"Biotin-Conjugate anti-human APRIL polyclonal antibody", "number":"AB119505-CMP04", "productcode":"" }, { "size":"1 x 150 µL", "name":"Streptavidin-HRP", "number":"AB119505-CMP08", "productcode":"" } ] } } }

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

+4°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

APRIL also known as TNFSF13 is a member of the tumor necrosis factor (TNF) ligand superfamily. This protein has a mass of approximately 31 kDa and undergoes incorporation into different cellular processes. APRIL is mainly expressed in cells of the immune system such as T cells B cells and myeloid cells. The protein plays a critical role in modulating various biological activities including cell survival proliferation and apoptosis.

Biological function summary

APRIL functions as a cytokine involved in immune regulation. The molecule acts independently or as part of a ligand-receptor interaction binding specifically to receptors like BCMA and TACI. This interaction influences B-cell maturation and differentiation driving immune responses. APRIL helps maintain homeostasis within the immune system ensuring appropriate immune activation and preventing overactive immune responses.

Pathways

APRIL coordinates with several immune-related pathways notably the B-cell signaling pathway and the NF-kB signaling pathway. These pathways are essential in immune system modulation influencing how the body responds to pathogens. APRIL interacts with proteins such as BAFF (B-cell activating factor) which share common receptors shaping the immune response through these pathways and ensuring proper immune cell function.

APRIL has connections with conditions like autoimmune diseases and certain lymphomas. Elevated levels of APRIL often occur in diseases like lupus contributing to abnormal B-cell activity and autoantibody production. Additionally its involvement in lymphomas links to excessive proliferation of B cells. Proteins such as BAFF and their receptors play a significant role in these conditions highlighting APRIL's impact on disease mechanisms and potential as a therapeutic target.

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제품 프로토콜

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타겟 정보

Cytokine that binds to TNFRSF13B/TACI and to TNFRSF17/BCMA. Plays a role in the regulation of tumor cell growth. May be involved in monocyte/macrophage-mediated immunological processes.

See full target information TNFSF13

대체 명칭 보기

CD256, APRIL, TALL2, ZTNF2, UNQ383/PRO715, TNFSF13, Tumor necrosis factor ligand superfamily member 13, A proliferation-inducing ligand, TNF- and APOL-related leukocyte expressed ligand 2, TNF-related death ligand 1, TALL-2, TRDL-1

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록and refine your search

Scientific reports 12:11219 PubMed35780200

2022

Protein and functional isoform levels and genetic variants of the BAFF and APRIL pathway components in systemic lupus erythematosus.

Applications

Unspecified application

Species

Unspecified reactive species

Pilar Ortiz-Aljaro,Marco Antonio Montes-Cano,José-Raúl García-Lozano,Virginia Aquino,Rosario Carmona,Javier Perez-Florido,Francisco José García-Hernández,Joaquín Dopazo,María Francisca González-Escribano

제품이 사용된 논문 모두 보기

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websiteProtocolBooklet

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human APRIL ELISA Kit

Sandwich ELISA - Human APRIL ELISA Kit (AB119505)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

결과 유형

Sensitivity

검출 범위

분석 플랫폼

보완 제품

Assay Kits

Assay Kits

Assay Kits

Assay Kits

Reactivity 정보

제품 세부 정보

Precision & recovery 정보

Precision

Recovery

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (1)

Product promise