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AB309427

Human beta-Glucuronidase ELISA Kit

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Human beta-Glucuronidase ELISA Kit is a Sandwich ELISA for the measurement of Human beta-Glucuronidase in Human in Biofluids, Cell Culture Media samples.
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Sandwich ELISA - Human beta-Glucuronidase ELISA Kit (AB309427)
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Supplier Data

Sandwich ELISA - Human beta-Glucuronidase ELISA Kit (AB309427)

Example of standard curve using ab309427. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

주요 정보

검출 방식

Colorimetric

샘플 타입

Serum, Plasma, Cell culture supernatant

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 0.18 ng/mL

검출 범위

0.18 - 40 ng/mL

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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보완 제품

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Proteins & Peptides

AB219458

Recombinant human beta glucuronidase (GUSB) protein (Active) (His tag C-Terminus)

Bioactive

SDS-PAGE - Recombinant human beta glucuronidase (GUSB) protein (Active) (His tag C-Terminus) (AB219458)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Human beta-Glucuronidase ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human beta-Glucuronidase in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human beta-Glucuronidase coated on a 96-well plate
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Recovery

[ { "sample": "Serum", "range": "104 - 129 %", "average": "= 111.7" }, { "sample": "Plasma", "range": "104 - 132 %", "average": "= 118.5" }, { "sample": "Cell culture media", "range": "80 - 125 %", "average": "= 108.7" } ]
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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab309427-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB309427-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB309427-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human beta-Glucuronidase/GUSB Protein Standard", "number":"AB309427-CMP10", "productcode":"" }, { "size":"1 x 200 µL", "name":"900X HRP-Streptavidin", "number":"AB309427-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human beta-Glucuronidase/GUSB Antibody-coated ELISA Plate", "number":"AB309427-CMP08", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB309427-CMP06", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB309427-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human beta-Glucuronidase/GUSB Detection Antibody", "number":"AB309427-CMP09", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
-20°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Beta glucuronidase also known as GUSB is an enzyme with several well-known names including b glucuronidase b-glucuronidase and beta-glucuronidase. It is a hydrolase enzyme with a molecular mass around 75 kDa. The enzyme is widely expressed in lysosomes of various tissues such as liver spleen and kidney. Mechanically beta-glucuronidase cleaves beta-D-glucuronic acid residues from glycosaminoglycans steroids and hormones an essential step for the degradation of glycosaminoglycans.
Biological function summary

Beta glucuronidase performs significant roles in cellular metabolism and molecule recycling. This enzyme aids in the breakdown and recycling of complex carbohydrates. By facilitating hydrolysis it helps in maintaining cellular homeostasis. Beta glucuronidase is not part of a complex; it functions independently within lysosomes. Its activity is vital for recycling endocytosed material and managing cellular turnover.

Pathways

Beta glucuronidase plays an important role in the lysosomal degradation pathway. This enzyme aids in glycosaminoglycan catabolism a process important for cellular waste disposal and recycling. Its activity connects with several proteins in the pathway notably those involved in lysosomal function such as Hexosaminidase. Beta glucuronidase's enzymatic function further integrates into the broader context of carbohydrate metabolism pathways enabling efficient breakdown of large carbohydrate molecules.

Mutations or deficiencies in beta glucuronidase lead to mucopolysaccharidosis type VII also called Sly syndrome. This lysosomal storage disorder results in the accumulation of undegraded glycosaminoglycans causing developmental delay skeletal abnormalities and other systemic issues. Additionally beta glucuronidase contributes to cancer biology where altered expressions can influence tumor progression. Proteins like glucocerebrosidase may interact indirectly impacting the disease's pathophysiology.
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제품 프로토콜

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타겟 정보

Plays an important role in the degradation of dermatan and keratan sulfates.
See full target information GUSB

대체 명칭 보기

Beta-glucuronidase, Beta-G1, GUSB
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websiteProtocolBooklet
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Product promise

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