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AB325367

Human C-Peptide (Insulin C-peptide) ELISA Kit, Chemiluminescent

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Human C-Peptide (Insulin C-peptide) ELISA Kit, Chemiluminescent is a 55 min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human C-Peptide (Insulin C-peptide) with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour
- Chemiluminescent detection allows a broad dynamic range: 0.46 - 1000 pg/mL with a sensitivity of 0.34 pg/mL
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Patent pending
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Sandwich ELISA - Human C-Peptide (Insulin C-peptide) ELISA Kit, Chemiluminescent (AB325367)
  • sELISA

Lab

Sandwich ELISA - Human C-Peptide (Insulin C-peptide) ELISA Kit, Chemiluminescent (AB325367)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human C-Peptide (Insulin C-peptide) ELISA Kit, Chemiluminescent (AB325367)
  • sELISA

Lab

Sandwich ELISA - Human C-Peptide (Insulin C-peptide) ELISA Kit, Chemiluminescent (AB325367)

Dynamic range comparison between the Chemiluminescent Human C-Peptide SimpleStep ELISA, colorimetric Human C-Peptide SimpleStep ELISA (ab260064), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Sandwich ELISA - Human C-Peptide (Insulin C-peptide) ELISA Kit, Chemiluminescent (AB325367)
  • sELISA

Lab

Sandwich ELISA - Human C-Peptide (Insulin C-peptide) ELISA Kit, Chemiluminescent (AB325367)

Interpolated concentration of native C-Peptide was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 12.5%, plasma (heparin) 12.5%, and urine 1 : 200. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human C-Peptide (Insulin C-peptide) ELISA Kit, Chemiluminescent (AB325367)
  • sELISA

Lab

Sandwich ELISA - Human C-Peptide (Insulin C-peptide) ELISA Kit, Chemiluminescent (AB325367)

Serum of eight individual healthy human male donors and serum of four congestive heart failure (CHF) donors was measured in duplicate. Average interpolated dilution factor corrected values (to neat sample) are plotted. The mean C-Peptide concentration of CHF serum was determined to be 5,450 pg/mL with a range of 1,892 – 7,335 pg/mL. The mean C-Peptide concentration of healthy donors was determined to be 669 pg/mL with a range of 17 – 1,467 pg/mL.

Sandwich ELISA - Human C-Peptide (Insulin C-peptide) ELISA Kit, Chemiluminescent (AB325367)
  • sELISA

Lab

Sandwich ELISA - Human C-Peptide (Insulin C-peptide) ELISA Kit, Chemiluminescent (AB325367)

Serum of eight individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean C-Peptide concentration was determined to be 669 pg/mL with a range of 17 – 1,467 pg/mL.

Sandwich ELISA - Human C-Peptide (Insulin C-peptide) ELISA Kit, Chemiluminescent (AB325367)
  • sELISA

Lab

Sandwich ELISA - Human C-Peptide (Insulin C-peptide) ELISA Kit, Chemiluminescent (AB325367)

Serum from three individual human male donors with type 2 diabetes was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean C-Peptide concentration in type 2 diabetes serum samples was determined to be 1.9 ng/mL with a range of 1.4 – 2.2 ng/mL.

Sandwich ELISA - Human C-Peptide (Insulin C-peptide) ELISA Kit, Chemiluminescent (AB325367)
  • sELISA

Lab

Sandwich ELISA - Human C-Peptide (Insulin C-peptide) ELISA Kit, Chemiluminescent (AB325367)

Standard curve comparison between the Chemiluminescent Human C-Peptide SimpleStep ELISA and colorimetric Human C-Peptide SimpleStep ELISA (ab260064) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

검출 방식

Luminescent

샘플 타입

Serum, Heparin Plasma, Cell culture media, Urine

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 0.34 pg/mL

검출 범위

0.46 - 1000 pg/mL

분석 소요 시간

55m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Human C-Peptide (Insulin C-peptide) ELISA Kit, Chemiluminescent (ab325367) is a rapid single-wash sandwich 55 min ELISA to measure Human C-Peptide (Insulin C-peptide) in Serum, Heparin Plasma, Cell culture media, Urine with a sensitivity of 0.34 pg/mL.

How the assay works

Human C-Peptide (Insulin C-peptide) ELISA Kit, Chemiluminescent (ab325367) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colormetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human C-Peptide (Insulin C-peptide) (ab325367) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-C Peptide antibody [EPR22500-5] - BSA and Azide free (Capture) ab259549
- Anti-C Peptide antibody [EPR22498-2] - BSA and Azide free (Detector) ab259550
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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.6" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.7" } ]
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Recovery

[ { "sample": "Serum", "range": "100 - 110 %", "average": "= 105" }, { "sample": "Heparin Plasma", "range": "85 - 102 %", "average": "= 92" }, { "sample": "Cell culture media", "range": "88 - 92 %", "average": "= 90" }, { "sample": "Urine", "range": "101 - 107 %", "average": "= 103" } ]
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제품 구성

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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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제품 프로토콜

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타겟 정보

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
See full target information C Peptide

대체 명칭 보기

Insulin
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websiteProtocolBooklet
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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