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AB224883

Human C1 inhibitor ELISA Kit

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Human C1 inhibitor ELISA Kit is a single-wash 90-min Simplestep used to quantify Human C1 inhibitor with a sensitivity of 10.9 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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ELISA - Human C1 inhibitor ELISA Kit (AB224883)
  • ELISA

Supplier Data

ELISA - Human C1 inhibitor ELISA Kit (AB224883)
Sandwich ELISA - Human C1 inhibitor ELISA Kit (AB224883)
  • sELISA

Supplier Data

Sandwich ELISA - Human C1 inhibitor ELISA Kit (AB224883)

Interpolated concentrations of native C1 inhibitor in human HepG2 cell culture supernatant.

HepG2 cells were cultured for 5 days in DMEM media containing 10% FBS and 1% NEAA. The concentrations of C1 inhibitor were measured in duplicate and interpolated from the C1 inhibitor standard curve and corrected for sample dilution. Undiluted samples are 18.75%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean C1 inhibitor concentration was determined to be 1,303 pg/mL.

Sandwich ELISA - Human C1 inhibitor ELISA Kit (AB224883)
  • sELISA

Supplier Data

Sandwich ELISA - Human C1 inhibitor ELISA Kit (AB224883)

Interpolated concentrations of native C1 inhibitor in human serum and plasma samples.

The concentrations of C1 inhibitor were measured in duplicates, interpolated from the C1 inhibitor standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 4000, plasma (citrate) 1 : 4000, plasma (EDTA) 1 : 4,000, and plasma (heparin) 1 : 8000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean C1 inhibitor concentration was determined to be 9,502 ng/mL in serum, 8,496 ng/mL in plasma (citrate), 4,285 ng/mL in plasma (EDTA), and 26,756 ng/mL in plasma (heparin).

Sandwich ELISA - Human C1 inhibitor ELISA Kit (AB224883)
  • sELISA

Supplier Data

Sandwich ELISA - Human C1 inhibitor ELISA Kit (AB224883)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean C1 inhibitor concentration was determined to be 12,259 pg/mL with a range of 39.7-20,572 pg/mL.

Sandwich ELISA - Human C1 inhibitor ELISA Kit (AB224883)
  • sELISA

Supplier Data

Sandwich ELISA - Human C1 inhibitor ELISA Kit (AB224883)

Example of human C1 inhibitor standard curve in Sample Diluent NS+1X Enhancer.

Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 10.9 pg/mL

검출 범위

31.25 - 2000 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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보완 제품

Reagents, Controls & Accessories

AB206978

Pre-Coated 96 Well Microplate (12 x 8 well strips) SimpleStep ELISA®

SimpleStep

ELISA - Pre-Coated 96 Well Microplate (12 x 8 well strips) SimpleStep ELISA® (AB206978)

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Assay Kits

AB230933

Mouse Thrombin ELISA Kit

Recombinant|SimpleStep

ELISA - Mouse Thrombin ELISA Kit (AB230933)

  • 0
  • 0
Assay Kits

AB202405

Human Prekallikrein 1B ELISA Kit

SimpleStep

ELISA - Human Prekallikrein 1B ELISA Kit (AB202405)

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Primary Antibodies

AB134918

Anti-SERPING1 antibody [EPR8015]

RabMAb|Recombinant

Western blot - Anti-SERPING1 antibody [EPR8015] (AB134918)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Human C1 inhibitor ELISA Kit ab224883 is a rapid single-wash 90-min Sandwich ELISA to measure Human C1 inhibitor in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 10.9 pg/mL.

How the assay works

Human C1 inhibitor SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human C1 inhibitor ELISA Kit ab224883 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-C1 inhibitor antibody [EPR20165-39] - BSA and Azide free (Capture) ab244940
- Anti-C1 inhibitor antibody [EPR20165-84] - BSA and Azide free (Detector) ab244940

C1 inhibitor, also known as C1 esterase inhibitor and Serpin G1, is a protease inhibitor belonging to the serpin superfamily, and is encoded by the gene SERPING1. C1 inhibitor is a 478 -amino acid containing protein, and is heavily glycosylated with an approximate 105 kDa molecular mass. The primary function of C1 inhibitor is the inhibition of the classical complement cascade to suppress spontaneous activation by forming an inactive complex with C1r and C1s proteases. In addition, C1 inhibitor also inactivates proteases of the fibrinolytic, clotting, and kinin pathways. The levels of C1 inhibitor in blood fluctuate during inflammation, and deficiencies of C1 inhibitor are associated with hereditary angioedema.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.4" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 9, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.9" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "89 - 95 %", "average": "= 92" }, { "sample": "Serum", "range": "111 - 114 %", "average": "= 113" }, { "sample": "EDTA Plasma", "range": "91 - 107 %", "average": "= 102" }, { "sample": "Heparin Plasma", "range": "92 - 108 %", "average": "= 100" }, { "sample": "Citrate plasma", "range": "101 - 112 %", "average": "= 106" } ]
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제품 구성

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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

C1 inhibitor also known as C1 esterase inhibitor or C1-INH is a serpin family protein that plays a significant role in inhibiting the complement system. This protein found in human serum has a molecular mass of about 104 kDa. C1 inhibitor is predominantly expressed in the liver and secreted into the bloodstream where it functions to regulate several proteolytic cascades. As a glycoprotein it also contains significant carbohydrate moieties that aid in its stability and function.
Biological function summary

As an inhibitor of the classical pathway of the complement system C1 inhibitor controls the activation of C1 esterase. This interaction prevents the uncontrolled activation of downstream components of the complement cascade which could result in tissue damage. C1 inhibitor also participates in regulating the kallikrein-kinin system preventing excessive inflammation. It achieves these roles not alone but as a part of complexes that interact with other proteins to maintain homeostasis within the immune system.

Pathways

The role of C1 inhibitor intersects with critical inflammatory modulatory pathways such as the complement pathway and the kallikrein-kinin system. These pathways are essential for proper immune response modulation preventing excessive inflammation and maintaining vascular permeability. C1-INH acts alongside proteases like kallikrein and proteins like C4 and C2 within these pathways to regulate the initiation and progression of the immune response during infections or insults.

Inadequate levels or functional defects in C1 inhibitor lead to hereditary angioedema a condition characterized by recurrent episodes of severe swelling. This disease highlights the importance of C1-INH in controlling vascular leakage and inflammation. Moreover C1 inhibitor has been implicated in autoimmune diseases like systemic lupus erythematosus where its regulatory kinetics with proteins such as C1q play a part in managing excessive complement activation. These associations highlight the important protective role of C1 inhibitor in health and disease.
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제품 프로토콜

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타겟 정보

Activation of the C1 complex is under control of the C1-inhibitor. It forms a proteolytically inactive stoichiometric complex with the C1r or C1s proteases. May play a potentially crucial role in regulating important physiological pathways including complement activation, blood coagulation, fibrinolysis and the generation of kinins. Very efficient inhibitor of FXIIa. Inhibits chymotrypsin and kallikrein.
See full target information SERPING1

대체 명칭 보기

C1IN, C1NH, SERPING1, Plasma protease C1 inhibitor, C1 Inh, C1Inh, C1 esterase inhibitor, C1-inhibiting factor, Serpin G1
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

PLoS pathogens 21:e1013107 PubMed40294075

2025

Deposition of complement regulators on the surface of Plasmodium falciparum merozoites depends on the immune status of the host.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Rosaria Bassi,Bogdan Cristinoi,Frank Buitenwerf,Mark Bergholt Cuadrado,Kasper Haldrup Björnsson,Melanie Rose Walker,Frederica Dedo Partey,Andrew B Ward,Michael Fokuo Ofori,Lea Barfod
제품이 사용된 논문 모두 보기
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websiteProtocolBooklet
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Product promise

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