Human C7 ELISA Kit
- Recombinant
- SimpleStep
- 각 태그의 뜻
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- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- sELISA
Supplier Data
Sandwich ELISA - Human C7 ELISA Kit (AB304938)
Interpolated concentrations of human C7 in serum, plasma (EDTA), plasma (heparin), and plasma (citrate)
Interpolated concentration of native C7 was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 10,000 serum, plasma (EDTA), plasma (heparin), and plasma (citrate). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
- sELISA
Supplier Data
Sandwich ELISA - Human C7 ELISA Kit (AB304938)
Interpolated concentrations of C7 in normal human serum donors
Serum of four individual healthy human female donors and four individual healthy Human male donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean C7 concentration was determined to be 42.6 µg/mL with a range of 29.0 - 55.4 µg/mL.
- sELISA
Supplier Data
Sandwich ELISA - Human C7 ELISA Kit (AB304938)
Example of human C7 standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.
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제품 세부 정보
Human C7 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of C7 protein in human serum, plasma, saliva and cell culture supernatant. Quantitate Human C7 with 18.846 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Precision
Recovery
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This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
C7 functions as an important facilitator in the innate immune response being an integral part of the membrane attack complex (MAC). This complex forms transmembrane channels in the cell membrane of pathogens leading to their lysis and destruction. Through its interaction with complement components like C5b and C6 C7 enables the insertion and stabilization of the MAC. This process is important for the immune system’s ability to eliminate pathogens quickly and effectively protecting the host from microbial invaders.
Pathways
The complement system pathway is the primary biological pathway involving C7. Within this pathway C7 interacts closely with complement proteins C5 and C6 contributing to the terminal pathway that leads to pathogen lysis. Besides it is linked with the classical and alternative complement pathways both of which converge at the activation of C5. Through these interactions and processes C7 works synergistically with other complement proteins to execute robust immune defense mechanisms.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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