AB287178

Human C9 ELISA Kit (Complement C9)

Recombinant
SimpleStep
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Human C9 ELISA Kit (Complement C9) is a single-wash 90-min Simplestep used to quantify Human C9 (Complement C9) with a sensitivity of 10.6 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

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Sandwich ELISA - Human C9 ELISA Kit (Complement C9) (AB287178)

sELISA

Supplier Data

Sandwich ELISA - Human C9 ELISA Kit (Complement C9) (AB287178)

Interpolated concentrations of human C9 in serum, plasma (heparin), plasma (EDTA), and plasma (citrate).

Interpolated concentration of native C9 was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 20,000 serum, plasma (heparin), plasma (EDTA), and plasma (citrate). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human C9 ELISA Kit (Complement C9) (AB287178)

Interpolated concentrations of human C9 in milk.

Interpolated concentration of native C9 was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 167 milk. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human C9 ELISA Kit (Complement C9) (AB287178)

Interpolated concentrations of C9 in normal human serum donors.

Serum of ten individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean C9 concentration was determined to be 73.8 μg/mL with a range of 28.5 – 99.3 μg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human C9 ELISA Kit (Complement C9) (AB287178)

Example of human C9 standard curve in Sample Diluent NS.

Example of human C9 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Heparin Plasma, Citrate plasma, Milk, Serum, EDTA Plasma

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 10.6 pg/mL

검출 범위

43.75 - 2800 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Human C9 ELISA kit (ab287178) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human C9 protein in human serum, plasma, and milk samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human C9 with 10.6 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 4, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.3" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.8" } ]

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Recovery

[ { "sample": "Milk", "range": "101 - 102 %", "average": "= 102" }, { "sample": "Serum", "range": "118 - 119 %", "average": "= 118" }, { "sample": "EDTA Plasma", "range": "110 - 117 %", "average": "= 114" }, { "sample": "Heparin Plasma", "range": "103 - 112 %", "average": "= 108" }, { "sample": "Citrate plasma", "range": "115 - 118 %", "average": "= 116" } ]

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제품 구성

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

+4°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

C9 also known as complement component 9 is a protein that plays an essential mechanical role in the complement system. It typically associates in the formation of the membrane attack complex (MAC). The C9 protein weighing around 71 kDa expresses in the liver and is secreted into the blood plasma. Alternative names like 'Cloud 9 capture' and 'C9 tag' often refer to research tools or motifs derived from the C9 protein rather than the protein itself. Researchers frequently study C9 in mouse models where its physiological roles can be explored.

Biological function summary

C9 participates in defending against pathogens. It joins other complement components like C5b C6 C7 and C8 to form the MAC complex which facilitates cell lysis by forming pores in the membrane of target cells. This action directly results in microbial cell destruction serving a critical immune function. C9 is not only part of the MAC complex but also holds significance due to its unique function as the last step in the formation of the pore influencing the efficacy of pathogen elimination.

Pathways

C9 functions primarily within the complement cascade which includes the classical and alternative pathways. Its role is in the terminal pathway segment that ultimately leads to the formation of the MAC and follows the activation of C5 which cleaves to produce C5b. This connection extends to proteins like C3 which is upstream in the cascade and significantly impacts the cascade's activation highlighting C9's function as an integral terminal component in these pathways.

Mutations or deficits in C9 relate to an increased susceptibility to infections particularly those caused by Neisseria species. This association results directly from its important role in the MAC formation. Additionally deficiency of C9 links to autoimmune disorders where the immune response may be improperly regulated due to compromised MAC formation. Other complement components like C6 and C8 may also relate to these diseases due to their collaboration with C9 in forming the MAC illustrating the interconnectedness of these proteins within the immune response framework.

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제품 프로토콜

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타겟 정보

Pore-forming component of the membrane attack complex (MAC), a multiprotein complex activated by the complement cascade, which inserts into a target cell membrane and forms a pore, leading to target cell membrane rupture and cell lysis (PubMed : 22832194, PubMed : 26841837, PubMed : 26841934, PubMed : 27052168, PubMed : 30552328, PubMed : 6177822, PubMed : 9212048, PubMed : 9634479). The MAC is initiated by proteolytic cleavage of C5 into complement C5b in response to the classical, alternative, lectin and GZMK complement pathways (PubMed : 39914456, PubMed : 39814882, PubMed : 9212048, PubMed : 9634479). The complement pathways consist in a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed : 9212048, PubMed : 9634479). Constitutes the pore-forming subunit of the MAC complex : during MAC assembly, C9 associates with the C5b8 intermediate complex, and polymerizes to complete the pore (PubMed : 26841934, PubMed : 30111885, PubMed : 30552328, PubMed : 34752492, PubMed : 4055801, PubMed : 6177822).

See full target information C9

대체 명칭 보기

Complement component C9, C9

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human C9 ELISA Kit (Complement C9)

Sandwich ELISA - Human C9 ELISA Kit (Complement C9) (AB287178)

Sandwich ELISA - Human C9 ELISA Kit (Complement C9) (AB287178)

Sandwich ELISA - Human C9 ELISA Kit (Complement C9) (AB287178)

Sandwich ELISA - Human C9 ELISA Kit (Complement C9) (AB287178)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

결과 유형

Sensitivity

검출 범위

분석 소요 시간

분석 플랫폼

보완 제품

Assay Kits

Assay Kits

Assay Kits

Assay Kits

Reactivity 정보

제품 세부 정보

Precision

Recovery

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

Product promise