AB320045

Human Calprotectin (S100A8/S100A9) ELISA Kit

Recombinant
SimpleStep
각 태그의 뜻

Be the first to review this product! 리뷰 제출

(1 출판물)

Human Calprotectin (S100A8/S100A9) ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Calprotectin (S100A8/S100A9) with a sensitivity of 13.624 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

6 이미지

Sandwich ELISA - Human Calprotectin (S100A8/S100A9) ELISA Kit (AB320045)

sELISA

Supplier Data

Sandwich ELISA - Human Calprotectin (S100A8/S100A9) ELISA Kit (AB320045)

Cross reactivity of related S100 proteins

50 ng/mL of recombinant human S100A8 and S100A9 were tested individually for cross-reactivity. Percent reactivity was determined by interpolating the protein concentration from the human Calprotectin standard curve and expressing the interpolated concentration as a percentage of the protein concentration tested.

sELISA

Supplier Data

Sandwich ELISA - Human Calprotectin (S100A8/S100A9) ELISA Kit (AB320045)

Interpolated concentrations of human Calprotectin in HL-60 cell extract and colon tissue extract

Interpolated concentration of native Calprotectin was measured in duplicate at different sample concentrations. Undiluted samples are as follows : HL-60 cell extract 500 ng/mL and colon tissue extract 200 ng/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Wash Buffer PT.

sELISA

Supplier Data

Sandwich ELISA - Human Calprotectin (S100A8/S100A9) ELISA Kit (AB320045)

Interpolated concentrations of human Calprotectin in serum, plasma (citrate), and saliva

Interpolated concentration of native Calprotectin was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 1 : 4,000, plasma (citrate) 1 : 4,000, and saliva 1 : 2,000. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Wash Buffer PT.

sELISA

Supplier Data

Sandwich ELISA - Human Calprotectin (S100A8/S100A9) ELISA Kit (AB320045)

Interpolated concentrations of human Calprotectin in plasma (EDTA), milk, stimulated PBMC supernatant, and urine

Interpolated concentration of native Calprotectin was measured in duplicate at different sample concentrations. Undiluted samples are as follows : plasma (EDTA) 1 : 1,000, milk 1 : 500, stimulated PBMC supernatant 1 : 2,000, and urine 1 : 500. Human PBMCs were cultured for 46 hours in the presence of 1.5% PHA-M. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Wash Buffer PT.

sELISA

Supplier Data

Sandwich ELISA - Human Calprotectin (S100A8/S100A9) ELISA Kit (AB320045)

Example of human Calprotectin standard curve in 1X Wash Buffer PT. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Calprotectin (S100A8/S100A9) ELISA Kit (AB320045)

Standard curve comparison between Human Calprotectin SimpleStep ELISA kit (ab320045) and traditional ELISA kit from leading competitor. The SimpleStep ELISA kit shows increased sensitivity.

주요 정보

검출 방식

Colorimetric

샘플 타입

Serum, Citrate plasma, EDTA Plasma, Cell culture supernatant, Urine, Saliva, Milk, Cell Lysate, Tissue Extracts

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 13.624 pg/mL

검출 범위

31.25 - 2000 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

제품 세부 정보

Human Calprotectin (S100A8/S100A9) SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Calprotectin (S100A8/S100A9) protein in Serum, Cit plasma, EDTA Plasma, Cell culture supernatant, Urine, Saliva, Milk, and Cell Lysate. Quantitate Human Calprotectin (S100A8/S100A9) with 13.624 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style

left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Plasma", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.5" }, { "reproducibilityType": "Inter", "sample": "Plasma", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.4" } ]

style

left-column

Recovery

[ { "sample": "Serum", "range": "98 - 112 %", "average": "= 107" }, { "sample": "Citrate plasma", "range": "101 - 109 %", "average": "= 105" }, { "sample": "EDTA Plasma", "range": "99 - 101 %", "average": "= 100" }, { "sample": "Cell culture supernatant", "range": "85 - 101 %", "average": "= 94" }, { "sample": "Urine", "range": "105 - 106 %", "average": "= 105" }, { "sample": "Saliva", "range": "85 - 105 %", "average": "= 95" }, { "sample": "Milk", "range": "104 - 119 %", "average": "= 113" }, { "sample": "Cell Lysate", "range": "96 - 110 %", "average": "= 105" }, { "sample": "Tissue Extracts", "range": "99 - 113 %", "average": "= 106" } ]

style

left-column

제품 구성

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab320045-10x96Tests", "assetComponentDetails": [ { "size":"10 x 1 Unit", "name":"Plate Seal", "number":"AB320045-CMP11" }, { "size":"2 x 50 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB320045-CMP05" }, { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB320045-CMP09" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB320045-CMP08" }, { "size":"10 x 96 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB320045-CMP10" }, { "size":"10 x 6 mL", "name":"Antibody Diluent BAR", "number":"AB320045-CMP04" }, { "size":"20 x 1 Vial", "name":"Human Calprotectin (S100A8/S100A9) Lyophilized Recombinant Protein", "number":"AB320045-CMP03" }, { "size":"10 x 600 µL", "name":"10X Human Calprotectin (S100A8/S100A9) Detector Antibody", "number":"AB320045-CMP02" }, { "size":"10 x 600 µL", "name":"10X Human Calprotectin (S100A8/S100A9) Capture Antibody", "number":"AB320045-CMP01" }, { "size":"2 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB320045-CMP06" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab320045-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB320045-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB320045-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB320045-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB320045-CMP08", "productcode":"" }, { "size":"1 x 96 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB320045-CMP10", "productcode":"" }, { "size":"2 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB320045-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Calprotectin (S100A8/S100A9) Capture Antibody", "number":"AB320045-CMP01", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent BAR", "number":"AB320045-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Calprotectin (S100A8/S100A9) Lyophilized Recombinant Protein", "number":"AB320045-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Calprotectin (S100A8/S100A9) Detector Antibody", "number":"AB320045-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB320045-CMP05", "productcode":"" } ] } } }

style

left-column

특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

+4°C

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

S100A9 and S100A8 also known as calprotectin or MRP8/14 when they form a complex together are small calcium-binding proteins with molecular masses of approximately 13 kDa and 11 kDa respectively. These proteins show high expression levels in neutrophils and monocytes serving important roles in the immune response. Both S100A9 and S100A8 display the ability to bind calcium ions changing their conformation and functional status. This calcium-induced structural change is essential for interacting with other proteins and cellular components.

Biological function summary

The S100A9 and S100A8 proteins regulate the inflammatory response by modulating immune cell activity. They function as heterodimers forming complexes that influence cell adhesion migration and the production of reactive oxygen species. Such activities are integral to both acute and chronic inflammation. The proteins achieve these effects by acting as antimicrobial agents and by regulating leukocyte recruitment to sites of infection or injury.

Pathways

S100A9 and S100A8 participate in key inflammation- and immune-related signaling cascades such as the NF-κB pathway and the TLR4 signaling pathway. In these contexts they interact with associated proteins like TLR4 and RAGE (Receptor for Advanced Glycation End-products). Their involvement in these pathways highlights their role in amplifying inflammatory signals during immune responses promoting the expression of pro-inflammatory cytokines.

S100A9 and S100A8 links with conditions like rheumatoid arthritis and inflammatory bowel disease. The upregulation of these proteins often correlates with increased inflammation and severity in such diseases. Moreover they connect with joint damage observed in rheumatoid arthritis through their interaction with RAGE. In inflammatory bowel disease their elevated levels serve as biomarkers for disease activity and guide therapeutic strategies aimed at modulating the immune response.

style

left-column

제품 프로토콜

style

left-column

타겟 정보

S100A8 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis and adhesion. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include : facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Also participates in regulatory T-cell differentiation together with CD69 (PubMed : 26296369). Activates NADPH-oxidase by facilitating the enzyme complex assembly at the cell membrane, transferring arachidonic acid, an essential cofactor, to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. The extracellular functions involve pro-inflammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities. Its pro-inflammatory activity includes recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER). Binding to TLR4 and AGER activates the MAP-kinase and NF-kappa-B signaling pathways resulting in the amplification of the pro-inflammatory cascade. Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn(2+) which is essential for microbial growth. Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3. Can regulate neutrophil number and apoptosis by an anti-apoptotic effect; regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK. Its role as an oxidant scavenger has a protective role in preventing exaggerated tissue damage by scavenging oxidants. Can act as a potent amplifier of inflammation in autoimmunity as well as in cancer development and tumor spread. The iNOS-S100A8/A9 transnitrosylase complex directs selective inflammatory stimulus-dependent S-nitrosylation of GAPDH and probably multiple targets such as ANXA5, EZR, MSN and VIM by recognizing a [IL]-x-C-x-x-[DE] motif; S100A8 seems to contribute to S-nitrosylation site selectivity.. (Microbial infection) Upon infection by human coronavirus SARS-CoV-2, may induce expansion of aberrant immature neutrophils in a TLR4-dependent manner.

See full target information S100A8

Additional targets

S100A9

대체 명칭 보기

CAGA, CFAG, MRP8, S100A8, Protein S100-A8, Calgranulin-A, Calprotectin L1L subunit, Cystic fibrosis antigen, Leukocyte L1 complex light chain, Migration inhibitory factor-related protein 8, S100 calcium-binding protein A8, Urinary stone protein band A, MRP-8, p8, CAGB, CFAG, MRP14, S100A9, Protein S100-A9, Calgranulin-B, Calprotectin L1H subunit, Leukocyte L1 complex heavy chain, Migration inhibitory factor-related protein 14, S100 calcium-binding protein A9, MRP-14, p14

style

left-column

제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell communication and signaling : CCS 23:141 PubMed40102920

2025

Increased melanin induces aberrant keratinocyte - melanocyte - basal - fibroblast cell communication and fibrogenesis by inducing iron overload and ferroptosis resistance in keloids.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangguang Shi,Xueyi Xia,Yang Xiao,Ying Zhang,Yiyi Gong,Yahui Chen,Chenyi Shi,Wei Wang,Jianlan Liu,Jia Huang,Mengguo Liu,Zhuoya Xu,Yanyun Ma,Mengkun Shi,Jiucun Wang,Wenyu Wu

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Calprotectin (S100A8/S100A9) ELISA Kit

Sandwich ELISA - Human Calprotectin (S100A8/S100A9) ELISA Kit (AB320045)

Sandwich ELISA - Human Calprotectin (S100A8/S100A9) ELISA Kit (AB320045)

Sandwich ELISA - Human Calprotectin (S100A8/S100A9) ELISA Kit (AB320045)

Sandwich ELISA - Human Calprotectin (S100A8/S100A9) ELISA Kit (AB320045)

Sandwich ELISA - Human Calprotectin (S100A8/S100A9) ELISA Kit (AB320045)

Sandwich ELISA - Human Calprotectin (S100A8/S100A9) ELISA Kit (AB320045)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

결과 유형

Sensitivity

검출 범위

분석 소요 시간

분석 플랫폼

보완 제품

Assay Kits

Assay Kits

Secondary Antibodies

Assay Kits

Reactivity 정보

제품 세부 정보

Precision

Recovery

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

Additional targets

대체 명칭 보기

제품이 사용된 논문 (1)

Product promise