Human Cardiac Troponin I ELISA Kit, Chemiluminescent

• sELISA

Lab

Sandwich ELISA - Human Cardiac Troponin I ELISA Kit, Chemiluminescent (AB326433)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

• sELISA

Lab

Sandwich ELISA - Human Cardiac Troponin I ELISA Kit, Chemiluminescent (AB326433)

Standard curve comparison between the Chemiluminescent Human Cardiac Troponin 1 SimpleStep ELISA and colorimetric Human Cardiac Troponin 1 SimpleStep ELISA (ab200016) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

• sELISA

Lab

Sandwich ELISA - Human Cardiac Troponin I ELISA Kit, Chemiluminescent (AB326433)

Dynamic range comparison between the Chemiluminescent Human Cardiac Troponin 1 SimpleStep ELISA and colorimetric Human Cardiac Troponin 1 SimpleStep ELISA (ab200016). The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

• sELISA

Supplier Data

Sandwich ELISA - Human Cardiac Troponin I ELISA Kit, Chemiluminescent (AB326433)

Sensitivity.

The MDD was determined by calculating the mean of zero standard replicates and adding 2 standard deviations then extrapolating the corresponding concentration.

• sELISA

Supplier Data

Sandwich ELISA - Human Cardiac Troponin I ELISA Kit, Chemiluminescent (AB326433)

Linearity of dilution.

Neat serum from ten individual healthy human female donors was measured in duplicate. All values were below the detectable range of the assay.

Native Cardiac Troponin 1 was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in 1X Cell Extraction Buffer PTR.

• sELISA

Supplier Data

Sandwich ELISA - Human Cardiac Troponin I ELISA Kit, Chemiluminescent (AB326433)

Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

Recombinant Cardiac Troponin 1 was spiked into the following biological samples and diluted in a 2-fold dilution series in Sample Diluent 50BP for serum and plasma samples and Sample Diluent NS for cell culture media samples.

Neat pooled serum and plasma (EDTA Heparin Citrate) samples from healthy donors was measured in duplicate. All values were below the detectable range of the assay.