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AB313522

Human Cathepsin E ELISA Kit

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Human Cathepsin E ELISA Kit is a Sandwich ELISA for the measurement of Human Cathepsin E in Human in Cell Culture Media, Biofluids samples.
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Sandwich ELISA - Human Cathepsin E ELISA Kit (AB313522)
  • sELISA

Supplier Data

Sandwich ELISA - Human Cathepsin E ELISA Kit (AB313522)

Example of standard curve using ab313522. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

주요 정보

검출 방식

Colorimetric

샘플 타입

Cell culture supernatant, Serum

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 14.5 pg/mL

검출 범위

14.5 - 4000 pg/mL

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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보완 제품

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Primary Antibodies

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Primary Antibodies

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Proteins & Peptides

AB185383

Recombinant Mouse Cathepsin E protein

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  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Human Cathepsin E ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Cathepsin E in serum (plasma is not recommended in this assay) and cell culture supernatants. This assay employs an antibody specific for human Cathepsin E coated on a 96-well plate.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human B7-2 (CD86); BAFF R (TNFRSF13C); Calcitonin; Calsyntenin-1; cIAP-2 (HIAP-1); Coagulation Factor VII; Complement MASP3; Endocan; EphA2; EphB4; Ephrin-A4; FGF-23; FGF-5; Flt-3 (Flk-2); GLP-1 (7-37 & 7-36- NH2); Glypican 2; GM-CSF R alpha; GP73 (GOLM1); HTRA2 (Omi); IL-20 R alpha; IL-4 R alpha; JAM-C; Luteinizing Hormone (LH); Matrilin-3; Meprin alpha (MEP1A); MSP R (Ron); N-Cadherin; Neprilysin-2 (MMEL1); NKp44; Pappalysin-1 (PAPP-A); Parathyroid hormone (PTH); Pepsinogen II (PGC); Peptide YY (PYY); Presenilin 1 (PSEN1); SOX2; TFPI-2; TRACP (ACP5); Trefoil factor 3 (TFF3); Ubiquitin+1.

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Recovery

[ { "sample": "Serum", "range": "71 - 112 %", "average": "= 87.15" }, { "sample": "Cell culture media", "range": "86 - 94 %", "average": "= 88.02" } ]
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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313522-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB313522-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"150X HRP-Streptavidin", "number":"AB313522-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB313522-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB313522-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human Cathepsin E Protein Standard", "number":"AB313522-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human Cathepsin E Antibody-coated ELISA Plate", "number":"AB313522-CMP08", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB313522-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human Cathepsin E Detection Antibody", "number":"AB313522-CMP09", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
-20°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Cathepsin E also known as CATE is an aspartic protease with a molecular mass of approximately 40 kDa. It functions within the cell to perform endopeptidase activity meaning it cleaves peptide bonds within proteins. Cathepsin E shows expression in the gastric mucosa and is prevalent in immune-related tissues such as the spleen and thymus. This enzyme operates optimally at acidic pH levels specializing in the breakdown of proteins in lysosomes.
Biological function summary

Cathepsin E plays a role in protein degradation and processing within lysosomal compartments. It associates with lysosomal and endosomal pathways participating actively in immune response regulation. Cathepsin E does not form part of a larger complex; however its activity can influence the function of other immune-related proteins by modifying antigen presentation.

Pathways

Cathepsin E engages with the major histocompatibility complex (MHC) class II pathway. This involvement assists in the processing of antigenic peptides for presentation to CD4+ T-cells connecting Cathepsin E to adaptive immune responses. Additionally it interacts with proteins such as HLA-DM which modulates the peptide loading on MHC class II molecules during immune surveillance.

Researchers link Cathepsin E to gastric cancer and Alzheimer's disease. In gastric cancer Cathepsin E overexpression can alter tumor microenvironments impacting disease progression. Furthermore in Alzheimer's disease Cathepsin E's activity correlates with amyloid precursor protein (APP) metabolism which can influence the accumulation of amyloid-beta plaques a hallmark of the disease process.
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제품 프로토콜

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타겟 정보

May have a role in immune function. Probably involved in the processing of antigenic peptides during MHC class II-mediated antigen presentation. May play a role in activation-induced lymphocyte depletion in the thymus, and in neuronal degeneration and glial cell activation in the brain.
See full target information Cathepsin E

대체 명칭 보기

Cathepsin E, CTSE
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websiteProtocolBooklet
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