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AB313523

Human Cathepsin H ELISA Kit

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Human Cathepsin H ELISA Kit is a Sandwich ELISA for the measurement of Human Cathepsin H in Human in Cell Culture Media, Biofluids samples.

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CPSB, CTSH, Pro-cathepsin H

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Sandwich ELISA - Human Cathepsin H ELISA Kit (AB313523)
  • sELISA

Supplier Data

Sandwich ELISA - Human Cathepsin H ELISA Kit (AB313523)

Example of standard curve using ab313523. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

주요 정보

검출 방식

Colorimetric

샘플 타입

Cell culture supernatant, Plasma, Serum

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 0.17 ng/mL

검출 범위

0.17 - 40 ng/mL

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Human Cathepsin H ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Cathepsin H in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human Cathepsin H coated on a 96-well plate.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human C1qTNF9, Carbonic Anhydrase VA, CANT1, Contactin-5, Chymotrypsin C/CTRC, Draxin, EphB2, EphB3, FABP8, Fgr, FKBP51, FUCA1, Galanin, GALNT10, Gastrokine 1, Glyoxalase II, HS3ST1, HS3ST3B1, Lin28, Lysyl Oxidase Homolog, LRRTM4, Methionine Aminopeptidase 1D, Matrilin-2, MCEMP1, Mcl-1, MDGA2, MEF2C, Methionine Aminopeptidase 2, Neurocan, Nogo-A, PCK1, PlGF-2, PON1, SALM4/LRFN3, Semaphorin 6C, SorCS2, ST3GAL1, ST8SIA1, Tsukushi/TSK.

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Recovery

[ { "sample": "Serum", "range": "105 - 123 %", "average": "= 113" }, { "sample": "Plasma", "range": "69 - 107 %", "average": "= 85.07" }, { "sample": "Cell culture media", "range": "80 - 142 %", "average": "= 99.46" } ]
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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313523-1x96Tests", "assetComponentDetails": [ { "size":"1 x 200 µL", "name":"250X HRP-Streptavidin", "number":"AB313523-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB313523-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB313523-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB313523-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human Cathepsin H Protein Standard", "number":"AB313523-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human Cathepsin H Antibody-coated ELISA Plate", "number":"AB313523-CMP08", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB313523-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human Cathepsin H Detection Antibody", "number":"AB313523-CMP09", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
-20°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Cathepsin H also known as CPSH is a lysosomal cysteine protease that plays a role in intracellular protein degradation. It has a molecular mass of approximately 28 kilodaltons. This enzyme is expressed across various tissues with high expression levels in liver kidney and spleen. The enzyme primarily functions at acidic pH levels within lysosomes where it acts to break down peptides into amino acids by removing terminal residues.
Biological function summary

As a member of the cathepsin family Cathepsin H contributes to the regulation of protein turnover and homeostasis. It performs a dual role as both an aminopeptidase and endopeptidase. In addition to its function in protein degradation the enzyme involves itself in antigen processing during immune responses. Reactivity of Cathepsin H in immune complexes shows its importance in shaping immune tolerance and responses.

Pathways

Cathepsin H interacts with the lysosomal degradation pathway and plays a role in the MHC class II-mediated antigen presentation. This pathway is pivotal for the regulation of immune responses. Relation to other proteins like Cathepsin L and Cathepsin B highlights Cathepsin H's complementary function in proteolytic processing within lysosomes. Its role and expression play an integral part in balancing protein synthesis and degradation cycles.

Cathepsin H associates closely with cancer and neurodegenerative diseases. Its activity can influence tumor progression and metastasis due to its role in extracellular matrix remodeling and cell migration. In neurodegenerative disorders such as Alzheimer's disease its dysregulated activity may contribute to neuronal cell death. Cathepsin H’s connection to proteins like amyloid precursor protein (APP) in Alzheimer's highlights the complex interplay between protease activity and disease mechanisms.
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제품 프로토콜

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타겟 정보

Important for the overall degradation of proteins in lysosomes.
See full target information CTSH
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websiteProtocolBooklet
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Product promise

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