Human CD127 ELISA Kit

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The Human CD127 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213799) is designed for the quantitative detection of Human CD127 in cell culture supernatants and serum.

The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A polyclonal antibody from goat specific for CD127 has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: E21-I262) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD127 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human CD127 amount of sample captured in plate.

CD127 (also known as interleukin-7 receptor or IL-7R) is a protein found on the surface of cells. It is mapped to 5p13.2. The protein encoded by this gene is a receptor for interleukin 7 (IL-7). The function of this receptor requires the interleukin 2 receptor, gamma chain (IL-2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukin 2, 4, 7, 9, and 15. CD127 has been shown to play a critical role in the development of immune cells called lymphocytes-specifically in a process known as V (D) J recombination. This protein is also found to control the accessibility of a region of the genome that contains the T-cell receptor gamma gene, by STAT5 and histone acetylation. What's more, CD127 antagonism is efficacious in treatment of EAE through its effects on Th17 cells and is a potential treatment for MS.