Human CD137 ELISA Kit
- Recombinant
- SimpleStep
- 각 태그의 뜻
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- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- sELISA
Unknown
Sandwich ELISA - Human CD137 ELISA Kit (AB229892)
Interpolated concentrations of spiked CD137 in human cell culture supernatant sample.
The concentrations of CD137 were measured in duplicates, interpolated from the CD137 standard curves and corrected for sample dilution. Undiluted samples are as follows : cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
- sELISA
Unknown
Sandwich ELISA - Human CD137 ELISA Kit (AB229892)
Interpolated concentrations of native CD137 in human stimulated THP-1 cell extract.
Extract based on 500 μg/mL extract load. The concentrations of CD137 were measured in duplicate and interpolated from the CD137 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD137 concentration was determined to be 537 pg/mL in Stimulated THP-1 cell extract, and undetectable in unstimulated THP-1 cell extract.
- sELISA
Unknown
Sandwich ELISA - Human CD137 ELISA Kit (AB229892)
Interpolated concentrations of spiked CD137 in human serum and plasma samples.
The concentrations of CD137 were measured in duplicates, interpolated from the CD137 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 25%, plasma (heparin) 50% and plasma (EDTA) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
- sELISA
Unknown
Sandwich ELISA - Human CD137 ELISA Kit (AB229892)
Example of human CD137 standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Unknown
Sandwich ELISA - Human CD137 ELISA Kit (AB229892)
Example of human CD137 standard curve in 1X Cell Extraction Buffer PTR.
Background-subtracted data values (mean +/- SD) are graphed.
보완 제품
Reactivity 정보
제품 세부 정보
Human CD137 ELISA Kit (ab229892) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD137 protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CD137 with 8.2 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Precision & recovery 정보
Precision
[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.8" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.4" } ]
Recovery
[ { "sample": "Serum", "range": "87 - 89 %", "average": "= 88" }, { "sample": "EDTA Plasma", "range": "82 - 85 %", "average": "= 84" }, { "sample": "Cell culture extracts", "range": "99 - 104 %", "average": "= 100" }, { "sample": "Heparin Plasma", "range": "80 - 82 %", "average": "= 81" }, { "sample": "Citrate plasma", "range": "86 - 92 %", "average": "= 90" }, { "sample": "Cell culture media", "range": "98 - 107 %", "average": "= 104" } ]
제품 구성
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보관 정보
추가 정보
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
CD137 plays an important role in T cell activation and survival. It is a member of a receptor-ligand system that enhances immune cell functions. CD137 is part of a receptor complex that when bound by its ligand CD137L promotes cell proliferation and cytokine production. It also supports the development of memory T cells contributing to the body's ability to mount long-term immune responses.
Pathways
CD137 takes part in the NF-kB signaling and MAPK pathways. These pathways are vital for regulating immune responses and inflammation. CD137 signaling through these pathways involves interaction with proteins such as TRAF1 and TRAF2 which help mediate downstream effects critical for cell survival and proliferation. These interactions enhance the body's defense against pathogens and tumors.
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com