AB45523

Human CD14 ELISA Kit

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Human CD14 ELISA Kit is a Sandwich ELISA for the measurement of Human CD14 in Human in Biofluids, Cell Culture Media, Cell/Tissue Extracts samples.

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Sandwich ELISA - Human CD14 ELISA Kit (AB45523)

sELISA

Lab

Sandwich ELISA - Human CD14 ELISA Kit (AB45523)

Sandwich ELISA -HuCD14 ELISA Kit.

Colorimetric standard curve : mean of duplicates (+/-SD) with background readings subtracted

sELISA

Lab

Sandwich ELISA - Human CD14 ELISA Kit (AB45523)

Sandwich ELISA - HuCD14 Elisa Kit.

hu CD14 measured in biologicals showing quantity (ng) per mL of tested sample

주요 정보

검출 방식

Colorimetric

샘플 타입

Plasma, Cell culture supernatant, Milk, Serum, Cell Lysate

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

< 6 ng/mL

검출 범위

6.89 - 220 ng/mL

분석 소요 시간

3h 45m

분석 플랫폼

Microplate

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Abcam's CD14 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CD14 in Human serum, plasma, buffered solutions or cell culture media.

A monoclonal antibody specific for CD14 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD14 concentrations and unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for CD14 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of CD14 present in the samples.

This kit will recognize both endogenous and recombinant Human CD14.

Get higher sensitivity in only 90 minutes with Human CD14 ELISA Kit (ab208983) from our SimpleStep ELISA^® range.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample A", "replicates": 8, "mean": "210.5 ng/mL", "standardDeviation": "1.55", "coefficientOfVariability": "0.73" }, { "reproducibilityType": "Inter", "sample": "Sample B", "replicates": 8, "mean": "24 ng/mL", "standardDeviation": "2", "coefficientOfVariability": "8.3" }, { "reproducibilityType": "Intra", "sample": "Sample A", "replicates": 8, "mean": "210.5 ng/mL", "standardDeviation": "2.6", "coefficientOfVariability": "1.23" }, { "reproducibilityType": "Intra", "sample": "Sample B", "replicates": 8, "mean": "23.4 ng/mL", "standardDeviation": "1.14", "coefficientOfVariability": "4.6" } ]

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제품 구성

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

+4°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CD14 also known as cluster of differentiation 14 is a glycoprotein with a molecular mass of approximately 55 kDa. This protein is expressed mainly on the surface of macrophages monocytes and to a lesser extent on neutrophils. CD14 exists in two forms: membrane-bound (mCD14) and soluble (sCD14). It functions as a co-receptor along with TLR4 (Toll-like receptor 4) and MD-2 for the detection of bacterial lipopolysaccharide (LPS) which is an important component of the outer membrane of Gram-negative bacteria.

Biological function summary

This glycoprotein plays a significant role in the innate immune system by recognizing pathogen-associated molecular patterns (PAMPs). It is part of a receptor complex where CD14 acts alongside LPS binding protein (LPS-R) TLR4 and MD-2 to facilitate pro-inflammatory signaling in response to microbial invasion. By binding to LPS CD14 initiates and amplifies the immune response leading to the production of cytokines and the recruitment of other immune cells to the site of infection.

Pathways

CD14 interacts with the toll-like receptor signaling pathway notably involving TLR4. Upon LPS detection CD14 assists in the activation of TLR4 triggering downstream signaling cascades such as NF-κB and MAPK pathways. These pathways result in the transcription of inflammatory cytokines and are essential for the host defense mechanism. The protein complex involving CD14 further interacts with adaptor proteins like MyD88 and TRIF facilitating signal propagation and the immune system's ability to respond to pathogens.

Researchers have linked CD14 to sepsis and atherosclerosis. High levels of soluble CD14 (sCD14) are often observed in patients with sepsis indicating its participation in excessive systemic inflammation. In atherosclerosis CD14's role in recognizing LPS contributes to chronic inflammation and the development of plaques in arteries. Through these diseases CD14 connects with TLR4 emphasizing its impact on inflammatory responses and its potential as a therapeutic target.

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제품 프로토콜

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타겟 정보

Coreceptor for bacterial lipopolysaccharide (PubMed : 1698311, PubMed : 23264655). In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed : 20133493, PubMed : 22265692, PubMed : 23264655). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed : 8612135). Acts as a coreceptor for TLR2 : TLR6 heterodimer in response to diacylated lipopeptides and for TLR2 : TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway (PubMed : 16880211). Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-) (PubMed : 23880187).

See full target information CD14

대체 명칭 보기

CD14, Monocyte differentiation antigen CD14, My23 antigen, Myeloid cell-specific leucine-rich glycoprotein

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Mediators of inflammation 2014:198413 PubMed24808634

2014

Circulating biomarkers of immune activation distinguish viral suppression from nonsuppression in HAART-treated patients with advanced HIV-1 subtype C infection.

Applications

Unspecified application

Species

Unspecified reactive species

Glen Malherbe,Helen C Steel,Sharon Cassol,Tulio de Oliveira,Christopher J Seebregts,Ronald Anderson,Edana Cassol,Theresa M Rossouw

제품이 사용된 논문 모두 보기

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websiteProtocolBooklet

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human CD14 ELISA Kit

Sandwich ELISA - Human CD14 ELISA Kit (AB45523)

Sandwich ELISA - Human CD14 ELISA Kit (AB45523)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

결과 유형

Sensitivity

검출 범위

분석 소요 시간

분석 플랫폼

보완 제품

Assay Kits

Assay Kits

Assay Kits

Assay Kits

Reactivity 정보

제품 세부 정보

Precision

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (1)

Product promise