Human CD30 ELISA Kit
- Recombinant
- SimpleStep
- 각 태그의 뜻
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- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- ELISA
Supplier Data
ELISA - Human CD30 ELISA Kit (AB236711)
- sELISA
Supplier Data
Sandwich ELISA - Human CD30 ELISA Kit (AB236711)
Serum from ten individual healthy human male donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD30 concentration was determined to be 398.1 pg/mL with a range of 170.5 – 597.9 pg/mL.
- sELISA
Supplier Data
Sandwich ELISA - Human CD30 ELISA Kit (AB236711)
Interpolated concentrations of native CD30 in human cell culture supernatant samples.
The concentrations of CD30 were measured in duplicates, interpolated from the CD30 standard curves and corrected for sample dilution. Undiluted samples are as follows : HDLM-2 supernatant 6.3%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD30 concentration was determined to be 23.1 ng/mL in HDLM-2 supernatant.
- sELISA
Supplier Data
Sandwich ELISA - Human CD30 ELISA Kit (AB236711)
Interpolated concentrations of native CD30 in human serum and plasma samples.
The concentrations of CD30 were measured in duplicates, interpolated from the CD30 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (heparin) 50%, and plasma (EDTA) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD30 concentration was determined to be 535.6 pg/ml in serum, 395.3 pg/ml in plasma (citrate), 434.5 pg/ml in plasma (heparin), and 512.9 pg/ml in plasma (EDTA).
- sELISA
Supplier Data
Sandwich ELISA - Human CD30 ELISA Kit (AB236711)
Example of human CD30 standard curve in Sample Diluent NS.
The CD30 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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How the assay works
Human CD30 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human CD30 ELISA Kit ab236711 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-CD30 antibody [EPR21828-36] - BSA and Azide free (Capture) ab245058
- Anti-CD30 antibody [EPR21828-217] - BSA and Azide free (Detector) ab245058
Precision
Recovery
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제품이 사용된 논문 (1)
Recent publications for all applications. Explore the 전체 목록 and refine your search
Cancer immunology, immunotherapy : CII 70:2353-2365 PubMed33527196
2021
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