- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- sELISA
Supplier Data
Sandwich ELISA - Human CD300a ELISA Kit (AB317784)
Interpolated concentrations of human CD300a TF-1 cell culture supernatant. Interpolated concentration of native CD300a was measured in duplicate at different Human sample concentrations. Undiluted samples are 100% TF-1 cell culture supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
- sELISA
Supplier Data
Sandwich ELISA - Human CD300a ELISA Kit (AB317784)
Interpolated concentrations of human CD300a in human serum in 96-well vs. 384-well plates. Interpolated concentration of native CD300a was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 50% human serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
- sELISA
Supplier Data
Sandwich ELISA - Human CD300a ELISA Kit (AB317784)
Interpolated concentrations of CD300a in normal human serum donors. Serum of eight individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean CD300a concentration was determined to be 2,115 pg/mL with a range of 863 - 3,518 pg/mL.
- sELISA
Supplier Data
Sandwich ELISA - Human CD300a ELISA Kit (AB317784)
Interpolated concentrations of human CD300a in serum, plasma – citrate plasma – EDTA, and plasma – heparin. Interpolated concentration of native CD300a was measured in duplicate at different Human sample concentrations. Undiluted samples are 50% serum, 50% plasma - citrate, 50% plasma - EDTA, and 50% plasma - heparin. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
- sELISA
Supplier Data
Sandwich ELISA - Human CD300a ELISA Kit (AB317784)
Example of human CD300a standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Human CD300a ELISA Kit (AB317784)
Example of human CD300a standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
보완 제품
Reactivity 정보
제품 세부 정보
Human CD300a SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD300a protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture supernatant. Quantitate Human CD300a with 4.063 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.
Precision
Recovery
제품 구성
특성 및 보관 정보
배송 시 보관 조건
적절한 단기 보관 조건
적절한 장기 보관 조건
보관 정보
타겟 정보
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com