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AB99990

Human CD40 ELISA Kit

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(4 제품이 사용된 논문 )

Human CD40 ELISA Kit is a Sandwich ELISA for the measurement of Human CD40 in Human in Biofluids, Cell Culture Media samples.
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Sandwich ELISA - Human CD40 ELISA Kit (AB99990)
  • sELISA

Supplier Data

Sandwich ELISA - Human CD40 ELISA Kit (AB99990)

Representative Standard Curve using ab99990

주요 정보

검출 방식

Colorimetric

샘플 타입

Plasma, Cell culture supernatant, Serum

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

< 50 pg/mL

검출 범위

54.87 - 40000 pg/mL

분석 플랫폼

Microplate

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보완 제품

Assay Kits

AB196268

Human CD40L ELISA Kit (TNFSF5)

Recombinant|SimpleStep

ELISA - Human CD40L ELISA Kit (TNFSF5) (AB196268)

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Assay Kits

AB289648

Human CD27 ELISA Kit

Recombinant|SimpleStep

ELISA - Human CD27 ELISA Kit (AB289648)

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Assay Kits

AB231926

Human OX40 ELISA Kit (TNFRSF4)

Recombinant|SimpleStep

Sandwich ELISA - Human OX40 ELISA Kit (TNFRSF4) (AB231926)

  • 0
  • 0
Assay Kits

AB181421

Human TNF alpha ELISA Kit

KO Validated|Recombinant|SimpleStep

Sandwich ELISA - Human TNF alpha ELISA Kit (AB181421)

  • 5
  • 4
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Abcam's CD40 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CD40 in serum, plasma, and cell culture supernatants.

This assay employs an antibody specific for Human CD40 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD40 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CD40 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD40 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Optimization may be required with urine samples.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "80 - 100 %", "average": "= 92.27" }, { "sample": "Serum", "range": "104 - 124 %", "average": "= 114.7" }, { "sample": "Plasma", "range": "78 - 100 %", "average": "= 90.19" } ]
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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab99990-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB99990-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D", "number":"AB99990-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB99990-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"CD40 Microplate (12 strips x 8 wells)", "number":"AB99990-CMP06", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB99990-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB99990-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-human CD40", "number":"AB99990-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"CD40 Standard Human (recombinant) (lyophilized)", "number":"AB99990-CMP07", "productcode":"" }, { "size":"1 x 200 µL", "name":"500X HRP-Streptavidin Concentrate", "number":"AB99990-CMP02", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
-20°C
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제품 프로토콜

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타겟 정보

Receptor for TNFSF5/CD40LG (PubMed : 31331973). Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion (By similarity).
See full target information CD40

대체 명칭 보기

CD40, TNFRSF5, Tumor necrosis factor receptor superfamily member 5, B-cell surface antigen CD40, Bp50, CD40L receptor, CDw40
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제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

eLife 10: PubMed34854378

2021

Single-cell-level protein analysis revealing the roles of autoantigen-reactive B lymphocytes in autoimmune disease and the murine model.

Applications

Unspecified application

Species

Unspecified reactive species

Takemichi Fukasawa,Ayumi Yoshizaki,Satoshi Ebata,Asako Yoshizaki-Ogawa,Yoshihide Asano,Atsushi Enomoto,Kiyoshi Miyagawa,Yutaka Kazoe,Kazuma Mawatari,Takehiko Kitamori,Shinichi Sato

Frontiers in immunology 9:1789 PubMed30131806

2018

Extracellular Vesicles Work as a Functional Inflammatory Mediator Between Vascular Endothelial Cells and Immune Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Baharak Hosseinkhani,Sören Kuypers,Nynke M S van den Akker,Daniel G M Molin,Luc Michiels

Scientific reports 7:7942 PubMed28801616

2017

Circulating CD40 and sCD40L Predict Changes in Renal Function in Subjects with Chronic Kidney Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Jeffrey X Xie,Helen Alderson,James Ritchie,Philip A Kalra,Yanmei Xie,Kaili Ren,Hanh Nguyen,Tian Chen,Pamela Brewster,Rajesh Gupta,Lance D Dworkin,Deepak Malhotra,Christopher J Cooper,Jiang Tian,Steven T Haller

Journal of hematology & oncology 7:64 PubMed25227919

2014

Highly efficient, in-vivo Fas-mediated apoptosis of B-cell lymphoma by hexameric CTLA4-FasL.

Applications

sELISA

Species

Human

Alexandra Aronin,Shira Amsili,Tatyana B Prigozhina,Kobi Tzdaka,Roy Shen,Leonid Grinmann,Fanny Szafer,Per Edebrink,Mari-Anne Rauvola,Noam Shani,Michal Dranitzki Elhalel
제품이 사용된 논문 모두 보기
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websiteProtocolBooklet
en
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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