Human CD62E ELISA Kit
- Recombinant
- SimpleStep
- 각 태그의 뜻
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Human CD62E ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CD62E with a sensitivity of 5.181 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
대체 명칭 보기
CD62E, ELAM1, SELE, E-selectin, CD62 antigen-like family member E, Endothelial leukocyte adhesion molecule 1, Leukocyte-endothelial cell adhesion molecule 2, ELAM-1, LECAM2
- sELISA
Supplier Data
Sandwich ELISA - Human CD62E ELISA Kit (AB303747)
Example of human CD62E standard curve in Sample Diluent NS.
Example of human CD62E standard curve. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Human CD62E ELISA Kit (AB303747)
Interpolated concentrations of human CD62E in serum, plasma (EDTA), plasma (citrate), and plasma (heparin).
Interpolated concentration of native CD62E was measured in duplicate at different sample concentrations. Undiluted samples are 2% serum, plasma (EDTA), plasma (citrate), and plasma (heparin). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
- sELISA
Supplier Data
Sandwich ELISA - Human CD62E ELISA Kit (AB303747)
Human CD62E competitor standard curve comparison.
Standard curve comparison between Human CD62E SimpleStep ELISA kit (ab303747) and traditional ELISA kit from leading competitor. The SimpleStep ELISA kit shows increased sensitivity.
- sELISA
Supplier Data
Sandwich ELISA - Human CD62E ELISA Kit (AB303747)
Interpolated concentrations of CD62E in normal human serum donors.
Serum of 4 individual healthy human female donors (donors 1-4) and 4 individual healthy human male donors (donors 5-8) was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean CD62E concentration was determined to be 35.7 ng/mL with a range of 16.67 – 71.30 ng/mL.
보완 제품
Reactivity 정보
제품 세부 정보
Human CD62E SimpleStep ELISA® kit (ab303747) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human CD62E protein in serum, plasma (EDTA), plasma (hep), plasma (cit) and cell culture media. Quantitate Human CD62E with 5.181 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Precision
Recovery
제품 구성
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보관 정보
추가 정보
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
E-selectin facilitates leukocyte adhesion by binding specific carbohydrate ligands on the surface of circulating immune cells. This binding is critical in the cascade of events that leads to leukocyte extravasation into tissues. E-selectin does not work in isolation rather forming part of a complex interaction with other cell adhesion molecules such as P-selectin and L-selectin. These interactions ensure precise control of cellular traffic during inflammatory responses.
Pathways
CD62E engages in the inflammatory signaling pathways including the NF-kB pathway. This pathway modulates the expression of E-selectin in response to pro-inflammatory cytokines like interleukin-1 and tumor necrosis factor-alpha. CD62E interacts with integrins on leukocytes and has downstream effects on cellular processes involved in immune response. Its cooperation with proteins like ICAM-1 and VCAM-1 further integrates it into a network of adhesion molecules maintaining vascular stability and immune surveillance.
타겟 정보
Product promise
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