AB45917

Human CD62L ELISA Kit (Selectin L)

Be the first to review this product! 리뷰 제출

(1 출판물)

Human CD62L ELISA Kit (Selectin L) is a Sandwich ELISA for the measurement of Human CD62L (Selectin L) in Human in Biofluids, Cell Culture Media samples.

1 이미지

Sandwich ELISA - Human CD62L ELISA Kit (Selectin L) (AB45917)

sELISA

Supplier Data

Sandwich ELISA - Human CD62L ELISA Kit (Selectin L) (AB45917)

Representative Standard Curve using ab45917

주요 정보

검출 방식

Colorimetric

샘플 타입

Plasma, Cell culture supernatant, Serum

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

< 1 ng/mL

검출 범위

1.9 - 60 ng/mL

분석 소요 시간

1h 45m

분석 플랫폼

Microplate

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

제품 세부 정보

Abcam's CD62L (Selectin L) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CD62L in Human serum, plasma, buffered solutions and cell culture medium.

A monoclonal antibody specific for CD62L has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD62L concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for CD62L are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of CD62L present in the samples.

This kit will recognize both endogenous and recombinant Human CD62L.

style

left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Sample A", "replicates": 15, "mean": "28 ng/mL", "standardDeviation": "1.3", "coefficientOfVariability": "4.6" }, { "reproducibilityType": "Intra", "sample": "Sample B", "replicates": 19, "mean": "9 ng/mL", "standardDeviation": "0.5", "coefficientOfVariability": "5.5" } ]

style

left-column

제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab45917-1x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Vial", "name":"CD62L Control", "number":"AB45917-CMP07" }, { "size":"1 x 11 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB45917-CMP10" }, { "size":"1 x 12 mL", "name":"HRP Diluent", "number":"AB45917-CMP03" }, { "size":"1 x 11 mL", "name":"Stop Reagent", "number":"AB45917-CMP02" }, { "size":"1 x 1 Unit", "name":"CD62L Microplate (12 x 8 well strips)", "number":"AB45917-CMP08" }, { "size":"1 x 10 mL", "name":"200X Wash Buffer", "number":"AB45917-CMP05" }, { "size":"1 x 7 mL", "name":"Biotinylated Antibody Diluent", "number":"AB45917-CMP01" }, { "size":"1 x 15 mL", "name":"10X Standard Diluent Buffer", "number":"AB45917-CMP06" }, { "size":"2 x 5 µL", "name":"Streptavidin-HRP", "number":"AB45917-CMP04" }, { "size":"1 x 0.4 mL", "name":"Biotinylated anti-CD62L antibody", "number":"AB45917-CMP11" }, { "size":"2 x 1 Vial", "name":"CD62L Standard", "number":"AB45917-CMP09" } ] }, "2x96Tests": { "sellingSize": "2 x 96 Tests", "publicAssetCode":"ab45917-2x96Tests", "assetComponentDetails": [ { "size":"4 x 1 Vial", "name":"CD62L Control", "number":"AB45917-CMP07", "productcode":"" }, { "size":"1 x 23 mL", "name":"HRP Diluent", "number":"AB45917-CMP03", "productcode":"" }, { "size":"2 x 11 mL", "name":"Stop Reagent", "number":"AB45917-CMP02", "productcode":"" }, { "size":"1 x 13 mL", "name":"Biotinylated Antibody Diluent", "number":"AB45917-CMP01", "productcode":"" }, { "size":"2 x 10 mL", "name":"200X Wash Buffer", "number":"AB45917-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"10X Standard Diluent Buffer", "number":"AB45917-CMP06", "productcode":"" }, { "size":"1 x 24 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB45917-CMP10", "productcode":"" }, { "size":"2 x 1 Unit", "name":"CD62L Microplate (12 x 8 well strips)", "number":"AB45917-CMP08", "productcode":"" }, { "size":"4 x 5 µL", "name":"Streptavidin-HRP", "number":"AB45917-CMP04", "productcode":"" }, { "size":"2 x 0.4 mL", "name":"Biotinylated anti-CD62L antibody", "number":"AB45917-CMP11", "productcode":"" }, { "size":"4 x 1 Vial", "name":"CD62L Standard", "number":"AB45917-CMP09", "productcode":"" } ] } } }

style

left-column

특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

+4°C

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CD62L also known as L-selectin is a cell adhesion molecule with a mass of approximately 74 kDa. It is part of the L-selectin family and is found on the surface of leukocytes including T cells B cells neutrophils and monocytes. Mouse models frequently study CD62L under the name "MEL-14". CD62L plays an important role in mediating the adhesion and rolling of leukocytes on blood vessel endothelial cells during the immune response. Its structure allows it to interact specifically with carbohydrates on endothelial cells facilitating the initial steps of leukocyte extravasation into tissues.

Biological function summary

CD62L is involved in the migration of leukocytes into lymphoid tissues and sites of inflammation. It is not known to be part of a larger protein complex; rather it functions independently on the cell membrane. CD62L also assists in the homing of naive lymphocytes to peripheral lymph nodes. The molecule regulates how immune cells enter tissues through high endothelial venules acting as a critical entry point for leukocyte trafficking.

Pathways

CD62L contributes significantly to the immune trafficking pathway and leukocyte adhesion cascade. It works closely with other adhesion molecules like CD44 and CD54 (ICAM-1) which are important within the context of cellular responses during inflammation. CD62L's function in these pathways ensures that immune cells are properly directed to areas where they are needed most whether that is secondary lymphoid organs or inflamed tissues.

CD62L expression and function have been associated with inflammatory conditions and certain immune-related pathologies such as multiple sclerosis and rheumatoid arthritis. Altered CD62L expression can affect lymphocyte homing potentially playing a role in the pathogenesis of these conditions. The role of CD62L is also linked to proteins like CD44 which can modulate cell interactions and have overlapping functions in disease mechanisms influencing disease progression or resolution.

style

left-column

제품 프로토콜

style

left-column

타겟 정보

Calcium-dependent lectin that mediates cell adhesion by binding to glycoproteins on neighboring cells (PubMed : 12403782, PubMed : 28011641, PubMed : 28489325). Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia (PubMed : 12403782, PubMed : 28011641).

See full target information SELL

대체 명칭 보기

CD62L, LNHR, LYAM1, SELL, L-selectin, CD62 antigen-like family member L, Leukocyte adhesion molecule 1, Leukocyte surface antigen Leu-8, Leukocyte-endothelial cell adhesion molecule 1, Lymph node homing receptor, TQ1, gp90-MEL, LAM-1, LECAM1

style

left-column

제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

PLoS computational biology 6: PubMed20885780

2010

Differentially expressed RNA from public microarray data identifies serum protein biomarkers for cross-organ transplant rejection and other conditions.

Applications

ELISA

Species

Human

Rong Chen,Tara K Sigdel,Li Li,Neeraja Kambham,Joel T Dudley,Szu-Chuan Hsieh,R Bryan Klassen,Amery Chen,Tuyen Caohuu,Alexander A Morgan,Hannah A Valantine,Kiran K Khush,Minnie M Sarwal,Atul J Butte

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human CD62L ELISA Kit (Selectin L)

Sandwich ELISA - Human CD62L ELISA Kit (Selectin L) (AB45917)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

결과 유형

Sensitivity

검출 범위

분석 소요 시간

분석 플랫폼

보완 제품

Assay Kits

Assay Kits

Assay Kits

Assay Kits

Reactivity 정보

제품 세부 정보

Precision

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (1)

Product promise