Human CD69 ELISA Kit
- Recombinant
- SimpleStep
- 각 태그의 뜻
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Human CD69 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CD69 with a sensitivity of 25.97 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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CD69, CLEC2C, Early activation antigen CD69, Activation inducer molecule, BL-AC/P26, C-type lectin domain family 2 member C, EA1, Early T-cell activation antigen p60, GP32/28, Leukocyte surface antigen Leu-23, MLR-3, AIM
- sELISA
Supplier Data
Sandwich ELISA - Human CD69 ELISA Kit (AB272464)
Interpolated concentrations of native CD69 in human serum and plasma (heparin) samples.
The concentrations of CD69 were measured in duplicates, interpolated from the CD69 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD69 concentration was determined to be 2,875.88 pg/mL in serum and 2,157.97 pg/mL in plasma (heparin).
- sELISA
Supplier Data
Sandwich ELISA - Human CD69 ELISA Kit (AB272464)
Serum from ten individual healthy human male donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD69 concentration was determined to be 6.44 ng/mL with a range of 0.31 – 49.92 ng/mL.
- sELISA
Supplier Data
Sandwich ELISA - Human CD69 ELISA Kit (AB272464)
Interpolated concentrations of native CD69 in human U937 cell extract based on a 375 μg/mL extract load.
The concentrations of CD69 were measured in duplicate and interpolated from the CD69 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD69 concentration was determined to be 3,432.14 pg/mL in U937 cell extract.
- sELISA
Supplier Data
Sandwich ELISA - Human CD69 ELISA Kit (AB272464)
Interpolated concentrations of native CD69 in stimulated and unstimulated human Daudi cell extract based on a 250 μg/mL extract load.
Stimulated Daudi cell extract samples were treated with 50 ng/mL PMA for 24 hours. The concentrations of CD69 were measured in duplicate and interpolated from the CD69 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD69 concentration was determined to be 6,406.69 pg/mL in PMA stimulated Daudi cell extract and non-detectable in unstimulated Daudi cell extract.
- sELISA
Supplier Data
Sandwich ELISA - Human CD69 ELISA Kit (AB272464)
Example of human CD69 standard curve in Sample Diluent NS.
The CD69 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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제품 세부 정보
Human CD69 ELISA kit (ab272464) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Neurofilament H protein in serum, plasma, cell culture supernatant and cell extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CD69 with 25.97 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
CD69 is an early activation marker for hematopoietic stem cells. It is expressed on activated T-cells, B-cells, NK cells, neutrophils, eosinophils, epidermal Langerhans cells and platelets. CD69 is involved in lymphocyte proliferation and signal transduction.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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추가 정보
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
CD69 functions as a signaling molecule during the immune response influencing both cell proliferation and cytokine production. It is a member of the C-type lectin superfamily and operates as a homodimer on the cell surface. CD69 helps modulate the migration of T cells to inflammatory sites by affecting sphingosine-1-phosphate receptor 1 (S1P1) downregulation directing their tissue retention and primary response roles.
Pathways
The protein plays an important role in the regulation of the NF-kB and JAK-STAT signaling pathways. These are important for immune cell communication and response and CD69 interacts with proteins like the IL-2 receptor complex in these pathways. Additionally its cross-linking can lead to altered expression of cytokines and chemokines impacting overall immune function.
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