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AB264621

Human CD70 ELISA Kit

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Human CD70 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CD70 with a sensitivity of 0.1 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
3 이미지
Sandwich ELISA - Human CD70 ELISA Kit (AB264621)
  • sELISA

Supplier Data

Sandwich ELISA - Human CD70 ELISA Kit (AB264621)

Representative image of Human CD70 ELISA Kit (ab264621)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Human CD70 ELISA Kit Capture Antibody

- 10X Human CD70 Detector Antibody

- 10X Human CD70 ELISA Kit Lyophilised Purified Protein (2 vials)

- Human CD70 ELISA Kit 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody Diluent 4BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

Sandwich ELISA - Human CD70 ELISA Kit (AB264621)
  • sELISA

Supplier Data

Sandwich ELISA - Human CD70 ELISA Kit (AB264621)

Interpolated concentrations of native CD70 in human U266B1 cell extract, Daudi cell extract, and PC-3 extract based on 800 μg/mL extract load.

The concentrations of CD70 were measured in duplicate and interpolated from the CD70 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD70 concentration was determined to be 13.35 ng/mL in U266B1 cell extract, 4.15 ng/mL in Daudi cell extract, and undetectable in PC-3 cell extract (data not shown).

Sandwich ELISA - Human CD70 ELISA Kit (AB264621)
  • sELISA

Supplier Data

Sandwich ELISA - Human CD70 ELISA Kit (AB264621)

Example of human CD70 standard curve in 1X Cell Extraction Buffer PTR.

The CD70 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Cell culture extracts, Cell Lysate

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 0.1 ng/mL

검출 범위

0.28 - 18 ng/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Human CD70 ELISA Kit (ab264621) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD70 protein in cell lysate and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CD70 with 0.1 ng/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

CD70 is a cytokine which acts as a ligand to CD27. The binding of CD70 to CD27 induces the proliferation of co-stimulated T-cells and enhances cytolytic T-cell generation. CD70-CD27 pathway plays an important role in the generation and maintenance of T-cell immunity during antiviral response.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.3" } ]
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Recovery

[ { "sample": "Cell culture extracts", "range": "116 - 123 %", "average": "= 120" }, { "sample": "Cell Lysate", "range": "121 - 126 %", "average": "= 123" } ]
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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab264621-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB264621-CMP11", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB264621-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB264621-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB264621-CMP12", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB264621-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB264621-CMP05", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB264621-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB264621-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB264621-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human CD70 Lyophilized Recombinant Protein", "number":"AB264621-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CD70 Capture Antibody", "number":"AB264621-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CD70 Detector Antibody", "number":"AB264621-CMP02", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CD70 also known as CD27 ligand or CD27L is a type II transmembrane protein with a molecular mass of approximately 30 kDa. It belongs to the tumor necrosis factor (TNF) superfamily and is encoded by the CD70 gene. The expression of CD70 occurs mainly on activated T and B cells dendritic cells and some NK cells but not on resting lymphocytes. In addition CD70 expression is observed in certain malignancies and inflammatory conditions.
Biological function summary

CD70 plays an essential role in the regulation of immune responses. It does this by binding to its receptor CD27 forming a receptor-ligand complex that triggers signaling pathways vital for T cell proliferation survival and differentiation. This interaction also influences the production of immunoglobulins by B cells. CD70-CD27 signaling is particularly important in shaping adaptive immunity enhancing the body's ability to respond to pathogens effectively.

Pathways

CD70 is integral in the activation of the NF-κB and MAPK pathways. These pathways are activated upon the interaction of CD70 with CD27 promoting cell survival proliferation and pro-inflammatory cytokine production. Other proteins related to CD70 through these pathways include TRAF2 and TRAF3 which are essential for downstream signaling events that modulate immune responses.

CD70 has been implicated in autoimmune diseases and certain cancers. For instance it plays a role in the pathogenesis of lupus by contributing to abnormal immune activation. Overexpression of CD70 is also associated with various cancers such as renal cell carcinoma where it can support tumor cell growth and immune evasion. Through these conditions CD70 interacts with proteins like BCL-2 in cancer affecting cell survival pathways while its interaction with CD27 influences the immune deregulation seen in lupus.
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제품 프로토콜

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타겟 정보

Expressed at the plasma membrane of B cells, it is the ligand of the CD27 receptor which is specifically expressed at the surface of T cells (PubMed : 28011863, PubMed : 28011864, PubMed : 8387892). The CD70-CD27 signaling pathway mediates antigen-specific T cell activation and expansion which in turn provides immune surveillance of B cells (PubMed : 28011863, PubMed : 28011864).
See full target information CD70

대체 명칭 보기

CD70, CD27L, CD27LG, TNFSF7, CD70 antigen, CD27 ligand, Tumor necrosis factor ligand superfamily member 7, CD27-L
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websiteProtocolBooklet
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Product promise

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