Human CD93 ELISA Kit
- Recombinant
- SimpleStep
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- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- sELISA
Supplier Data
Sandwich ELISA - Human CD93 ELISA Kit (AB235636)
Serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD93 concentration was determined to be 230.4 ng/mL with a range of 134.8 – 719.6 ng/mL.
- sELISA
Supplier Data
Sandwich ELISA - Human CD93 ELISA Kit (AB235636)
Interpolated concentrations of native CD93 in human serum and plasma samples.
The concentrations of CD93 were measured in duplicates, interpolated from the CD93 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1%, plasma (citrate) 2%, plasma (heparin) 2% and plasma (EDTA) 1%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD93 concentration was determined to be 191.2 ng/mL in serum, 189.7 ng/mL in plasma (citrate), 166.8 ng/ml in plasma (heparin), and 255.0 ng/mL in plasma (EDTA).
- sELISA
Supplier Data
Sandwich ELISA - Human CD93 ELISA Kit (AB235636)
Human CD93 Competitor Std Curve Comparison.
Standard curve comparison between human CD93 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
- sELISA
Supplier Data
Sandwich ELISA - Human CD93 ELISA Kit (AB235636)
Interpolated concentrations of native CD93 in human urine and LPS-treated THP-1 supernatant samples.
The concentrations of CD93 were measured in duplicates, interpolated from the CD93 standard curves and corrected for sample dilution. Undiluted samples are as follows : urine 50% and LPS-treated THP-1 supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD93 concentration was determined to be 1.2 ng/mL in urine, and 6.3 ng/ml in treated THP-1 supernatant.
- sELISA
Supplier Data
Sandwich ELISA - Human CD93 ELISA Kit (AB235636)
Example of human CD93 standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
보완 제품
Reactivity 정보
제품 세부 정보
Human CD93 ELISA Kit (ab235636) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD93 protein in edta plasma, hep plasma, serum, urine, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CD93 with 13.4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Precision
Recovery
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보관 정보
추가 정보
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
CD93 contributes to cellular processes through its role in the clearance of apoptotic cells and immune surveillance. It also partakes in angiogenesis facilitating the growth of new blood vessels. CD93 often functions as part of a larger complex in binding with other molecules to support cellular communication. By modulating the immune response CD93 ensures efficient removal of cellular debris maintaining homeostasis and preventing autoimmune reactions.
Pathways
CD93 operates within the innate immune response and complement system pathways. It closely interacts with other proteins such as C1q which is part of the complement activation pathway. This interaction supports the clearance of immune complexes and cellular debris. By facilitating these processes CD93 aids in maintaining the balance between immune activation and tolerance.
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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