JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB283982

Human CENP-B ELISA Kit

Be the first to review this product! 리뷰 제출

|

(0 출판물)

Human CENP-B ELISA Kit is a Sandwich ELISA for the measurement of Human CENP-B in Human in Cell/Tissue Extracts samples.

대체 명칭 보기

Major centromere autoantigen B, Centromere protein B, CENP-B, CENPB

1 이미지
Sandwich ELISA - Human CENP-B ELISA Kit (AB283982)
  • sELISA

Supplier Data

Sandwich ELISA - Human CENP-B ELISA Kit (AB283982)

Example of Human CENPB standard curve. Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Cell Lysate

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 0.42 ng/mL

검출 범위

0.625 - 40 ng/mL

분석 소요 시간

4h

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

style
left-column

보완 제품

Primary Antibodies

AB259855

Anti-CENPB antibody [EPR24047-64]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CENPB antibody [EPR24047-64] (AB259855)

  • 0
  • 0
Proteins & Peptides

AB275796

Recombinant Human CENPB protein (His tag)

SDS-PAGE - Recombinant Human CENPB protein (His tag) (AB275796)

  • 0
  • 0
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

제품 세부 정보

The Human CENP-B ELISA Kit (ab283982) is designed for detection of CENP-B in human cell lysate samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures human CENP-B in approximately 4 hours.

A polyclonal antibody specific for human CENP-B has been pre-coated onto a 96-well microplate with removable strips. CENP-B in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human CENP-B, which is recognized by a streptavidin-peroxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The colour development is stopped and the intensity of the colour is measured.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 60, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.1" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 60, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6" } ]
style
left-column

제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab283982-1x96Tests", "assetComponentDetails": [ { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB283982-CMP10", "productcode":"" }, { "size":"1 x 2 mL", "name":"1X Standard Diluent", "number":"AB283982-CMP02", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Diluent M Concentrate", "number":"AB283982-CMP01", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB283982-CMP09", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB283982-CMP04", "productcode":"" }, { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB283982-CMP06", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB283982-CMP08", "productcode":"" }, { "size":"1 x 120 µL", "name":"50X Biotinylated Human CENP-B Antibody", "number":"AB283982-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human CENP-B Microplate", "number":"AB283982-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"CENP-B Standard", "number":"AB283982-CMP03", "productcode":"" } ] } } }
style
left-column

특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
Multi
보관 정보
Please refer to protocols
style
left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CENP-B also known as Centromere Protein B is a protein with a molecular weight of approximately 80 kDa. It binds to the centromeric DNA and plays a critical role in centromere formation and function. CENP-B is heavily expressed in dividing cells particularly in tissues where cell proliferation is high such as bone marrow and epithelial tissues. Researchers often use tools like anti-CENP-B antibodies and CENP-B ELISA kits to study its expression in various experimental settings.
Biological function summary

CENP-B participates in the assembly of the centromere protein complex contributing to the stability and maintenance of the centromere structure during cell division. It acts as a foundation for kinetochore nucleation ensuring proper chromosome segregation. CENP-B is involved in the formation of the CENP-A nucleosome complex important for accurate chromosomal alignment and separation.

Pathways

CENP-B is essential in the mitotic spindle assembly and chromosome segregation pathways. This protein interacts with CENP-A a variant histone which integrates into centromeric nucleosomes to initiate the formation of functional kinetochores. Additionally CENP-B interacts with CENP-C facilitating the recruitment of other centromere-associated proteins needed for chromosome dynamics during mitosis.

CENP-B is primarily associated with autoimmune disorders such as systemic sclerosis (scleroderma). Patients with these conditions often produce anti-CENP-B antibodies which serve as diagnostic markers. Moreover CENP-B's dysregulation links to chromosomal instability a contributor to cancer. Research has shown connections between CENP-B and related proteins like CENP-A in the context of chromosomal aneuploidies and malignancies.
style
left-column

제품 프로토콜

style
left-column

타겟 정보

Interacts with centromeric heterochromatin in chromosomes and binds to a specific 17 bp subset of alphoid satellite DNA, called the CENP-B box (PubMed : 11726497). May organize arrays of centromere satellite DNA into a higher-order structure which then directs centromere formation and kinetochore assembly in mammalian chromosomes (Probable).
See full target information CENPB
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com