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AB99994

Human CNTF ELISA Kit

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Human CNTF ELISA Kit is a Sandwich ELISA for the measurement of Human CNTF in Human in Biofluids, Cell Culture Media samples.
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Sandwich ELISA - Human CNTF ELISA Kit (AB99994)
  • sELISA

Supplier Data

Sandwich ELISA - Human CNTF ELISA Kit (AB99994)

Representative Standard Curve using ab99994

Sandwich ELISA - Human CNTF ELISA Kit (AB99994)
  • sELISA

Supplier Data

Sandwich ELISA - Human CNTF ELISA Kit (AB99994)

Representative Standard Curve using ab99994

주요 정보

검출 방식

Colorimetric

샘플 타입

Plasma, Cell culture supernatant

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

< 0.2 ng/mL

검출 범위

0.823 - 200 ng/mL

분석 플랫폼

Microplate

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보완 제품

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Reagents, Controls & Accessories

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Primary Antibodies

AB267430

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Assay Kits

AB178013

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Abcam's CNTF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CNTF in plasma and cell culture supernatants. (Human CNTF concentration is pretty low in normal plasma, it may not be detected in this assay).

This assay employs an antibody specific for Human CNTF coated on a 96-well plate. Standards and samples are pipetted into the wells and CNTF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CNTF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CNTF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

We have not been able to detect endogenous Human CNTF in normal serum with ab99994, only in serum spiked with Human CNTF.

Optimization may be required with urine samples.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "84 - 104 %", "average": "= 95.45" }, { "sample": "Plasma", "range": "83 - 104 %", "average": "= 93.28" } ]
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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab99994-1x96Tests", "assetComponentDetails": [ { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB99994-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB99994-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB99994-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"CNTF Microplate (12 strips x 8 wells)", "number":"AB99994-CMP07", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB99994-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB99994-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human CNTF Standard (lyophilized)", "number":"AB99994-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human CNTF", "number":"AB99994-CMP09", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X HRP-Streptavidin Concentrate", "number":"AB99994-CMP02", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
-20°C
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제품 프로토콜

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타겟 정보

CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.
See full target information CNTF

대체 명칭 보기

Ciliary neurotrophic factor, CNTF
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Stem cells (Dayton, Ohio) 36:844-855 PubMed29476565

2018

Human Periodontal Ligament-Derived Stem Cells Promote Retinal Ganglion Cell Survival and Axon Regeneration After Optic Nerve Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Ling-Ping Cen,Tsz Kin Ng,Jia-Jian Liang,Xi Zhuang,Xiaowu Yao,Gary Hin-Fai Yam,Haoyu Chen,Herman S Cheung,Mingzhi Zhang,Chi Pui Pang
제품이 사용된 논문 모두 보기
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websiteProtocolBooklet
en
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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