JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB213764

Human COMP ELISA Kit

Human COMP ELISA 키트

5

(2 리뷰들)

|

(1 출판물)

Human COMP ELISA Kit is a Sandwich ELISA for the measurement of Human COMP in Human in Cell/Tissue Extracts, Cell Culture Media, Biofluids samples.

대체 명칭 보기

Cartilage oligomeric matrix protein, COMP, Thrombospondin-5, TSP5

1 이미지
Sandwich ELISA - Human COMP ELISA Kit (AB213764)
  • sELISA

Supplier Data

Sandwich ELISA - Human COMP ELISA Kit (AB213764)

Human COMP ELISA Kit (ab213764) Standard Curve.

주요 정보

검출 방식

Colorimetric

샘플 타입

Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

< 10 pg/mL

검출 범위

156 - 10000 pg/mL

분석 소요 시간

3h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

style
left-column

보완 제품

Assay Kits

AB213754

Human Aggrecan ELISA Kit

Sandwich ELISA - Human Aggrecan ELISA Kit (AB213754)

  • 0
  • 0
Assay Kits

AB222511

Mouse C Reactive Protein ELISA Kit

Recombinant|SimpleStep

ELISA - Mouse C Reactive Protein ELISA Kit (AB222511)

  • 0
  • 0
Assay Kits

AB219046

Human Fibronectin ELISA Kit

Recombinant|SimpleStep

ELISA - Human Fibronectin ELISA Kit (AB219046)

  • 0
  • 0
Assay Kits

AB269371

Human MMP3 ELISA Kit

Recombinant|SimpleStep

ELISA - Human MMP3 ELISA Kit (AB269371)

  • 0
  • 0
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

제품 세부 정보

The Human COMP Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213764) is designed for the quantitative measurement of Human COMP in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A polyclonal antibody from goat specific for COMP has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: Q21-A757) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for COMP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human COMP amount of sample captured in plate.

Cartilage oligomeric matrix protein is a protein that in humans is encoded by the COMP gene. The sequences of rat and bovine COMP indicate that it is a member of the thrombospondin gene family. By Southern blot analysis of a somatic cell hybrid DNA panel and by isotopic in situ hybridization, human COMP gene was mapped to 19p13.1, and the murine COMP gene was mapped to the central region of mouse chromosome 8 by use of an interspecific backcross mapping panel. COMP is a marker of cartilage turnover.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 24, "mean": "1.13 ng/mL", "standardDeviation": "0.07", "coefficientOfVariability": "6.2" }, { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 24, "mean": "3.08 ng/mL", "standardDeviation": "0.166", "coefficientOfVariability": "5.4" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 24, "mean": "6.23 ng/mL", "standardDeviation": "0.324", "coefficientOfVariability": "5.2" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 16, "mean": "1.02 ng/mL", "standardDeviation": "0.052", "coefficientOfVariability": "5.1" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 16, "mean": "3.12 ng/mL", "standardDeviation": "0.131", "coefficientOfVariability": "4.2" }, { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 16, "mean": "6.19 ng/mL", "standardDeviation": "0.254", "coefficientOfVariability": "4.1" } ]
style
left-column

제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab213764-1x96Tests", "assetComponentDetails": [ { "size":"1 x 20 mL", "name":"Wash Buffer (25X)", "number":"AB213764-CMP11", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Stop Solution", "number":"AB213764-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"ABC Diluent Buffer", "number":"AB213764-CMP03", "productcode":"" }, { "size":"4 x 1 Unit", "name":"Adhesive Plate Seal", "number":"AB213764-CMP06", "productcode":"" }, { "size":"1 x 30 mL", "name":"Sample Diluent Buffer", "number":"AB213764-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"Antibody Diluent Buffer", "number":"AB213764-CMP09", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Color Developing Agent", "number":"AB213764-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human COMP coated Microplate (12 x 8 wells)", "number":"AB213764-CMP08", "productcode":"" }, { "size":"1 x 100 µL", "name":"Avidin-Biotin-Peroxidase Complex (ABC)", "number":"AB213764-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized recombinant Human COMP standard", "number":"AB213764-CMP01", "productcode":"" }, { "size":"1 x 100 µL", "name":"Biotinylated anti- Human COMP antibody", "number":"AB213764-CMP07", "productcode":"" } ] } } }
style
left-column

특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
-20°C
style
left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The Cartilage Oligomeric Matrix Protein (COMP) also known as oligomeric protein or COMP cartilage protein is a structural component found in the extracellular matrix of cartilage tissues. This matrix protein has a mass of approximately 524 kDa and is predominantly expressed in cartilage particularly hyaline cartilage. COMP forms pentameric structures which make it an important supporting element in the stability and resilience of cartilage. Researchers often use techniques like COMP ELISA to quantify its levels in biological samples.
Biological function summary

COMP interacts with other extracellular matrix components to enhance structural integrity and function of cartilage tissue. It performs significant roles in the organization and maintenance of the matrix tethering fibrillar proteins and supporting cellular interaction. COMP is not typically part of a large complex but works closely with collagens and other matrix proteins to form a robust and flexible extracellular environment.

Pathways

COMP participates in pathways associated with cartilage formation and turnover. It engages in chondrocyte-mediated processes that are vital for endochondral ossification and cartilage repair. Within these pathways it interacts with proteins such as collagen type II and decorin which facilitate the organization and mineralization of cartilage matrix. These interactions highlight the protein's importance in maintaining cartilage architecture and function.

COMP plays a role in chondrodysplasias and osteoarthritis. Mutations in the COMP gene can lead to skeletal disorders like pseudoachondroplasia and multiple epiphyseal dysplasia causing growth and developmental anomalies. In osteoarthritis alterations in COMP levels may serve as an indicator of cartilage degradation. COMP's relationships with proteins like aggrecan and matrilin-3 in these contexts highlight its involvement in pathologies that affect cartilage structure and health.
style
left-column

제품 프로토콜

style
left-column

타겟 정보

Plays a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. Can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors (PubMed : 16051604, PubMed : 16542502). Could play a role in the pathogenesis of osteoarthritis (PubMed : 16542502). Potent suppressor of apoptosis in both primary chondrocytes and transformed cells. Suppresses apoptosis by blocking the activation of caspase-3 and by inducing the IAP family of survival proteins (BIRC3, BIRC2, BIRC5 and XIAP) (PubMed : 17993464). Essential for maintaining a vascular smooth muscle cells (VSMCs) contractile/differentiated phenotype under physiological and pathological stimuli. Maintains this phenotype of VSMCs by interacting with ITGA7 (By similarity).
See full target information COMP
style
left-column

제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Frontiers in molecular biosciences 11:1252058 PubMed38584705

2024

Serum proteomic profiling of precancerous gastric lesions and early gastric cancer reveals signatures associated with systemic inflammatory response and metaplastic differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Yueqing Gong,Yaxin Lou,Xiurui Han,Keyan Chen,Yang Zhao,Hejun Zhang,Jing Zhang,Ying Xiong,Weiwei Fu,Shigang Ding
제품이 사용된 논문 모두 보기
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com