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AB326748

Human Complement C2 ELISA Kit, Chemiluminescent

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Human Complement C2 ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human Complement C2 with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 45.72 - 100000 pg/ml with a sensitivity of 32.5 pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending
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Sandwich ELISA - Human Complement C2 ELISA Kit, Chemiluminescent (AB326748)
  • sELISA

Lab

Sandwich ELISA - Human Complement C2 ELISA Kit, Chemiluminescent (AB326748)

Standard curve comparison between the Chemiluminescent Human Complement C2 SimpleStep ELISA and colorimetric Human Complement C2 SimpleStep ELISA (ab254501) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Complement C2 ELISA Kit, Chemiluminescent (AB326748)
  • sELISA

Lab

Sandwich ELISA - Human Complement C2 ELISA Kit, Chemiluminescent (AB326748)

Dynamic range comparison between the Chemiluminescent Human Complement C2 SimpleStep ELISA, colorimetric Human Complement C2 SimpleStep ELISA (ab254501), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Sandwich ELISA - Human Complement C2 ELISA Kit, Chemiluminescent (AB326748)
  • sELISA

Supplier Data

Sandwich ELISA - Human Complement C2 ELISA Kit, Chemiluminescent (AB326748)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Complement C2 concentration was determined to be 19.67 μg/mL with a range of 12.10 – 27.26 μg/mL.

Sandwich ELISA - Human Complement C2 ELISA Kit, Chemiluminescent (AB326748)
  • sELISA

Supplier Data

Sandwich ELISA - Human Complement C2 ELISA Kit, Chemiluminescent (AB326748)

Interpolated concentrations of native Complement C2 in human cell culture supernatant, milk, and female saliva samples.

The concentrations of Complement C2 were measured in duplicates, interpolated from the Complement C2 standard curves and corrected for sample dilution. Undiluted samples are as follows : HepG2 cell culture supernatant 6.25%, milk 12.5%, and female saliva 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Complement C2 concentration was determined to be 117.47 ng/mL in HepG2 cell culture supernatant, 117.91 ng/mL in milk, and 22.87 ng/mL in female saliva.

Sandwich ELISA - Human Complement C2 ELISA Kit, Chemiluminescent (AB326748)
  • sELISA

Supplier Data

Sandwich ELISA - Human Complement C2 ELISA Kit, Chemiluminescent (AB326748)

Interpolated concentrations of native Complement C2 in human serum and plasma samples.

The concentrations of Complement C2 were measured in duplicates, interpolated from the Complement C2 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 4,000, plasma (citrate) 1 : 2,000, plasma (heparin) 1 : 1,000, and plasma (EDTA) 1 : 1,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Complement C2 concentration was determined to be 27.00 μg/mL in serum, 15.46 μg/mL in plasma (citrate), 11.89 μg/ml in plasma (heparin), and 12.51 μg/ml in plasma (EDTA).

주요 정보

검출 방식

Luminescent

샘플 타입

Cell culture supernatant, Serum, Milk, EDTA Plasma, Saliva, Heparin Plasma, Citrate plasma, Cell culture media

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 32.5 pg/mL

검출 범위

45.72 - 100000 pg/mL

분석 소요 시간

55m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

제품 세부 정보

Human Complement C2 ELISA Kit, Chemiluminescent (ab326748) is a rapid single-wash sandwich 55-min ELISA to measure Human Complement C2 in Cell culture supernatant, Serum, Milk, EDTA Plasma, Saliva, Heparin Plasma, Citrate plasma, Cell culture media with a sensitivity of 32.5 pg/mL.

How the assay works

Human Complement C2 ELISA Kit, Chemiluminescent (ab326748) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Complement C2 (ab326748) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-C2 antibody [EPR22452-69] - BSA and Aside free (Capture) ab259713
- Anti-C2 antibody [EPR22452-177] - BSA and Aside free (Detector) ab259714

Precision

[ { "reproducibilityType": "Intra", "sample": "Plasma", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6" }, { "reproducibilityType": "Inter", "sample": "Plasma", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 5.5" } ]

Recovery

[ { "sample": "Cell culture supernatant", "range": "107 - 116 %", "average": "= 116" }, { "sample": "Serum", "range": "94 - 107 %", "average": "= 107" }, { "sample": "Milk", "range": "104 - 114 %", "average": "= 114" }, { "sample": "EDTA Plasma", "range": "96 - 121 %", "average": "= 121" }, { "sample": "Saliva", "range": "113 - 116 %", "average": "= 116" }, { "sample": "Heparin Plasma", "range": "96 - 102 %", "average": "= 102" }, { "sample": "Citrate plasma", "range": "101 - 108 %", "average": "= 108" }, { "sample": "Cell culture media", "range": "94 - 108 %", "average": "= 108" } ]

제품 구성

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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C

제품 프로토콜

타겟 정보

Component C2 which is part of the classical pathway of the complement system is cleaved by activated factor C1 into two fragments : C2b and C2a. C2a, a serine protease, then combines with complement factor C4b to generate the C3 or C5 convertase.
See full target information C2

대체 명칭 보기

Complement C2, C3/C5 convertase, C2
websiteProtocolBooklet
en

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