Human Complement C2 ELISA Kit, Chemiluminescent
- SimpleStep Ignite™ ELISA
- Recombinant
- 각 태그의 뜻
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- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 45.72 - 100000 pg/ml with a sensitivity of 32.5 pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending
- sELISA
Lab
Sandwich ELISA - Human Complement C2 ELISA Kit, Chemiluminescent (AB326748)
Standard curve comparison between the Chemiluminescent Human Complement C2 SimpleStep ELISA and colorimetric Human Complement C2 SimpleStep ELISA (ab254501) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Lab
Sandwich ELISA - Human Complement C2 ELISA Kit, Chemiluminescent (AB326748)
Dynamic range comparison between the Chemiluminescent Human Complement C2 SimpleStep ELISA, colorimetric Human Complement C2 SimpleStep ELISA (ab254501), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.
- sELISA
Supplier Data
Sandwich ELISA - Human Complement C2 ELISA Kit, Chemiluminescent (AB326748)
Serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Complement C2 concentration was determined to be 19.67 μg/mL with a range of 12.10 – 27.26 μg/mL.
- sELISA
Supplier Data
Sandwich ELISA - Human Complement C2 ELISA Kit, Chemiluminescent (AB326748)
Interpolated concentrations of native Complement C2 in human cell culture supernatant, milk, and female saliva samples.
The concentrations of Complement C2 were measured in duplicates, interpolated from the Complement C2 standard curves and corrected for sample dilution. Undiluted samples are as follows : HepG2 cell culture supernatant 6.25%, milk 12.5%, and female saliva 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Complement C2 concentration was determined to be 117.47 ng/mL in HepG2 cell culture supernatant, 117.91 ng/mL in milk, and 22.87 ng/mL in female saliva.
- sELISA
Supplier Data
Sandwich ELISA - Human Complement C2 ELISA Kit, Chemiluminescent (AB326748)
Interpolated concentrations of native Complement C2 in human serum and plasma samples.
The concentrations of Complement C2 were measured in duplicates, interpolated from the Complement C2 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 4,000, plasma (citrate) 1 : 2,000, plasma (heparin) 1 : 1,000, and plasma (EDTA) 1 : 1,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Complement C2 concentration was determined to be 27.00 μg/mL in serum, 15.46 μg/mL in plasma (citrate), 11.89 μg/ml in plasma (heparin), and 12.51 μg/ml in plasma (EDTA).
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How the assay works
Human Complement C2 ELISA Kit, Chemiluminescent (ab326748) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.
Assay Specificity
Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human Complement C2 (ab326748) protocol summary
1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-C2 antibody [EPR22452-69] - BSA and Aside free (Capture) ab259713
- Anti-C2 antibody [EPR22452-177] - BSA and Aside free (Detector) ab259714
Precision
Recovery
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com