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AB243686

Human Complement Factor D ELISA Kit

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Human Complement Factor D ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Complement Factor D with a sensitivity of 2.6 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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Sandwich ELISA - Human Complement Factor D ELISA Kit (AB243686)
  • sELISA

Supplier Data

Sandwich ELISA - Human Complement Factor D ELISA Kit (AB243686)

Interpolated concentrations of native Complement Factor D in human serum and plasma samples.

The concentrations of Complement Factor D were measured in duplicates, interpolated from the Complement Factor D standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 1,000, plasma (citrate) 1 : 1,000, plasma (EDTA) 1 : 1,000and plasma (heparin) 1 : 1,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Complement Factor D concentration was determined to be 944.1 ng/mL in serum, 721.8 ng/mL in plasma (citrate), 860.5 ng/mL in plasma (EDTA), and 321.7 ng/mL in plasma (heparin).

Sandwich ELISA - Human Complement Factor D ELISA Kit (AB243686)
  • sELISA

Supplier Data

Sandwich ELISA - Human Complement Factor D ELISA Kit (AB243686)

Human Complement Factor D Standard Curve Comparison.

Standard Curve comparison between human Complement Factor D SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

Sandwich ELISA - Human Complement Factor D ELISA Kit (AB243686)
  • sELISA

Supplier Data

Sandwich ELISA - Human Complement Factor D ELISA Kit (AB243686)

Serum from ten individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Complement Factor D concentration was determined to be 975.9 ng/mL with a range of 556.8 – 1,603.5 ng/mL.

Sandwich ELISA - Human Complement Factor D ELISA Kit (AB243686)
  • sELISA

Unknown

Sandwich ELISA - Human Complement Factor D ELISA Kit (AB243686)

Example of human Complement Factor D standard curve in Sample Diluent NS.

Sandwich ELISA - Human Complement Factor D ELISA Kit (AB243686)
  • sELISA

Supplier Data

Sandwich ELISA - Human Complement Factor D ELISA Kit (AB243686)

Interpolated concentrations of native Complement Factor D in human urine and cell culture supernatant samples.

The concentrations of Complement Factor D were measured in duplicates, interpolated from the Complement Factor D standard curves and corrected for sample dilution. Undiluted samples are as follows : male urine 100%, female urine 50%, and U937 supernatant 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Complement Factor D concentration was determined to be 958.8 pg/mL in male urine, 494.7 pg/mL in female urine and 4,734.2 pg/mL in U937 supernatant.

Sandwich ELISA - Human Complement Factor D ELISA Kit (AB243686)
  • sELISA

Supplier Data

Sandwich ELISA - Human Complement Factor D ELISA Kit (AB243686)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Complement Factor D concentration was determined to be 1,142.5 ng/mL with a range of 867.1 – 1,958.8 ng/mL.

주요 정보

검출 방식

Colorimetric

샘플 타입

Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 2.6 pg/mL

검출 범위

15.63 - 1000 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Human Complement Factor D ELISA Kit (ab243686) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Complement Factor D protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Complement Factor D with 2.6 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.3" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.4" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "87 - 102 %", "average": "= 96" }, { "sample": "Serum", "range": "92 - 105 %", "average": "= 98" }, { "sample": "EDTA Plasma", "range": "84 - 102 %", "average": "= 93" }, { "sample": "Urine", "range": "83 - 90 %", "average": "= 88" }, { "sample": "Heparin Plasma", "range": "82 - 102 %", "average": "= 95" }, { "sample": "Citrate plasma", "range": "90 - 100 %", "average": "= 94" } ]
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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab243686-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB243686-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB243686-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB243686-CMP10", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB243686-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB243686-CMP06", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB243686-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB243686-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Complement Factor D Lyophilized Recombinant Protein", "number":"AB243686-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Complement Factor D Capture Antibody", "number":"AB243686-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Complement Factor D Detector Antibody", "number":"AB243686-CMP03", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Factor D also known as complement factor D or CFD protein is a serine protease involved in the alternative pathway of the complement system. It plays a mechanical role by cleaving factor B when it is bound to C3b forming the active C3 convertase C3bBb. This enzyme is relatively small with a mass of approximately 28.5 kDa. It is mainly expressed in adipose tissue but it can also be found in the blood plasma.
Biological function summary

Factor D aids the immune system's ability to destroy pathogens. As part of the alternative complement pathway it acts independently and does not associate with larger protein complexes. Its activation of the alternative pathway leads to direct lysis of pathogens therefore contributing to innate immunity.

Pathways

Factor D participates specifically in the complement activation pathway particularly in the innate immune response. It works closely with other proteins in the complement system such as factor B and C3. This pathway enhances the opsonization and removal of microbes and damaged cells acting in parallel with the classical pathway of the complement system.

Abnormal function or regulation of factor D can relate to chronic inflammation and age-related macular degeneration. Disorders involving factor D may involve excess complement activation leading to tissue damage. The role of factor D in these diseases often connects it to other key complement components such as C3 and factor B which can also become problematic when dysregulated.
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제품 프로토콜

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타겟 정보

Serine protease that initiates the alternative pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed : 21205667, PubMed : 22362762, PubMed : 6769474, PubMed : 874324, PubMed : 9748277). In contrast to other complement pathways (classical, lectin and GZMK) that are directly activated by pathogens or antigen-antibody complexes, the alternative complement pathway is initiated by the spontaneous hydrolysis of complement C3 (PubMed : 21205667, PubMed : 22362762, PubMed : 6769474, PubMed : 874324). The alternative complement pathway acts as an amplification loop that enhances complement activation by mediating the formation of C3 and C5 convertases (PubMed : 21205667, PubMed : 22362762, PubMed : 6769474, PubMed : 874324). Activated CFD cleaves factor B (CFB) when the latter is complexed with complement C3b, activating the C3 convertase of the alternative pathway (PubMed : 21205667, PubMed : 6769474, PubMed : 874324, PubMed : 9748277).
See full target information CFD

대체 명칭 보기

DF, PFD, CFD, Complement factor D, Adipsin, C3 convertase activator, Properdin factor D
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Frontiers in cardiovascular medicine 9:1003282 PubMed36172581

2022

Exosomes derived from pericardial adipose tissues attenuate cardiac remodeling following myocardial infarction by Adipsin-regulated iron homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Wanrong Man,Xinglong Song,Zhenyu Xiong,Jing Gu,Jie Lin,Xiaoming Gu,Duan Yu,Congye Li,Mengyuan Jiang,Xuebin Zhang,Zhi Yang,Yang Cao,Yan Zhang,Xiaofei Shu,Dexi Wu,Haichang Wang,Gang Ji,Dongdong Sun
제품이 사용된 논문 모두 보기
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websiteProtocolBooklet
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Product promise

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