AB325998

Human CTHRC1 ELISA Kit, Chemiluminescent

Recombinant
SimpleStep Ignite™ ELISA
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Human CTHRC1 ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human CTHRC1 with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 18.29 - 40000 pg/ml with a sensitivity of 4.01 pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending

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Sandwich ELISA - Human CTHRC1 ELISA Kit, Chemiluminescent (AB325998)

sELISA

Lab

Sandwich ELISA - Human CTHRC1 ELISA Kit, Chemiluminescent (AB325998)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

sELISA

Lab

Sandwich ELISA - Human CTHRC1 ELISA Kit, Chemiluminescent (AB325998)

Interpolated concentration of native CTHRC1 in human serum, plasma and cell culture supernatant samples was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, and SK-MEL28 cell culture supernatant 4%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CTHRC1 concentration was determined to be 9,486 pg/mL in serum, 10,463 pg/mL in plasma (citrate) and 140,962 pg/mL in SK-MEL28 cell culture supernatant. Sample dilutions are made in Sample Diluent NS.

sELISA

Lab

Sandwich ELISA - Human CTHRC1 ELISA Kit, Chemiluminescent (AB325998)

Standard curve comparison between the Chemiluminescent Human CTHRC1 SimpleStep ELISA and colorimetric Human CTHRC1 SimpleStep ELISA (ab274399) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Human CTHRC1 ELISA Kit, Chemiluminescent (AB325998)

Dynamic range comparison between the Chemiluminescent Human CTHRC1 SimpleStep ELISA, colorimetric Human CTHRC1 SimpleStep ELISA (ab274399), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

주요 정보

검출 방식

Luminescent

샘플 타입

Cell culture supernatant, Citrate plasma, Serum

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 4.01 pg/mL

검출 범위

18.29 - 40000 pg/mL

분석 소요 시간

55m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Human CTHRC1 ELISA Kit, Chemiluminescent (ab325998) is a rapid single-wash sandwich 55-min ELISA to measure Human CTHRC1 in Cell culture supernatant, Citrate Plasma, Serum with a sensitivity of 4.01 pg/mL.

How the assay works

Human CTHRC1 ELISA Kit, Chemiluminescent (ab325998) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human CTHRC1 (ab325998) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-CTHRC1 antibody [EPR22851-144] - BSA and Aside free (Capture) ab274446
- Anti-CTHRC1 antibody [EPR22851-215] - BSA and Aside free (Detector) ab274447

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Precision

[ { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.6" }, { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.9" } ]

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Recovery

[ { "sample": "Serum", "range": "91 - 101 %", "average": "= 97" }, { "sample": "Citrate plasma", "range": "95 - 103 %", "average": "= 100" }, { "sample": "Cell culture supernatant", "range": "99 - 101 %", "average": "= 100" } ]

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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab325998-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB325998-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human CTHRC1 Lyophilized Recombinant Protein", "number":"AB325998-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CTHRC1 Capture Antibody", "number":"AB325998-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB325998-CMP09", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB325998-CMP06", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent B", "number":"AB325998-CMP08", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent A", "number":"AB325998-CMP07", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB325998-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CTHRC1 Detector Antibody", "number":"AB325998-CMP02", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB325998-CMP05", "productcode":"" } ] } } }

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

+4°C

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제품 프로토콜

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타겟 정보

May act as a negative regulator of collagen matrix deposition.

See full target information CTHRC1

대체 명칭 보기

UNQ762/PRO1550, CTHRC1, Collagen triple helix repeat-containing protein 1

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websiteProtocolBooklet

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human CTHRC1 ELISA Kit, Chemiluminescent

Sandwich ELISA - Human CTHRC1 ELISA Kit, Chemiluminescent (AB325998)

Sandwich ELISA - Human CTHRC1 ELISA Kit, Chemiluminescent (AB325998)

Sandwich ELISA - Human CTHRC1 ELISA Kit, Chemiluminescent (AB325998)

Sandwich ELISA - Human CTHRC1 ELISA Kit, Chemiluminescent (AB325998)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

결과 유형

Sensitivity

검출 범위

분석 소요 시간

분석 플랫폼

보완 제품

Proteins & Peptides

Assay Kits

Primary Antibodies

Assay Kits

Reactivity 정보

제품 세부 정보

Precision

Recovery

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

제품 프로토콜

타겟 정보

대체 명칭 보기

Product promise