Human CXCL3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CXCL3 with a sensitivity of 1.1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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GRO3, GROG, SCYB3, CXCL3, C-X-C motif chemokine 3, GRO-gamma(1-73), Growth-regulated protein gamma, Macrophage inflammatory protein 2-beta, GRO-gamma, MIP2-beta
- ELISA
Supplier Data
ELISA - Human CXCL3 ELISA Kit (AB234574)
- sELISA
Supplier Data
Sandwich ELISA - Human CXCL3 ELISA Kit (AB234574)
Interpolated concentration of native CXCL3 was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 400 PHA-M stimulated PBMC cell culture media. PBMC were cultured in the presence of 1.5% PHA-M for 46 hours. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
- sELISA
Supplier Data
Sandwich ELISA - Human CXCL3 ELISA Kit (AB234574)
Interpolated concentration of native CXCL3 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : 1 : 100 unstimulated PBMC cell culture media and 1 : 400 PHA-M stimulated PBMC cell culture media. PBMC were cultured in the presence or absence of 1.5% PHA-M for 46 hours. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
- sELISA
Supplier Data
Sandwich ELISA - Human CXCL3 ELISA Kit (AB234574)
Serum of eight individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean CXCL3 concentration was determined to be 69.29 pg/mL with a range of 18.26 – 119.71 pg/mL.
- sELISA
Supplier Data
Sandwich ELISA - Human CXCL3 ELISA Kit (AB234574)
Interpolated concentration of native CXCL3 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 50%, plasma (citrate) 25%, and plasma (EDTA) 12.5%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
- sELISA
Supplier Data
Sandwich ELISA - Human CXCL3 ELISA Kit (AB234574)
Example of human CXCL3 standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
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Reactivity 정보
제품 세부 정보
How the assay works
Human CXCL3 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human CXCL3 ELISA Kit ab234574 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-GRO gamma antibody [EPR20154-21] - BSA and Azide free (Capture) ab244842
- Anti-GRO gamma antibody [EPR20154-30] - BSA and Azide free (Detector) ab245032
Precision
Recovery
제품 구성
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적절한 단기 보관 조건
적절한 장기 보관 조건
보관 정보
추가 정보
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
GRO gamma functions as a chemokine that guides neutrophils by binding to its receptor CXCR2. It participates in inflammatory processes and is produced in response to stimuli such as bacterial products and pro-inflammatory cytokines. GRO gamma together with its close family members forms a network facilitating the immune cell recruitment and activation at infection sites. Also this chemokine is included in larger chemokine complexes influencing not just immune cells but also modulating angiogenesis and cellular matrix restructuring.
Pathways
GRO gamma significantly contributes to the chemokine signaling pathway and the NF-kB signaling pathway. In these pathways it interacts tightly with proteins like CXCR2 which is essential for the signaling cascade that results in immune cell migration. It also engages with the MAPK pathways impacting cell proliferation and apoptosis. These involvements make GRO gamma vital for orchestrating the inflammation response in humans.
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Recent publications for all applications. Explore the 전체 목록 and refine your search
Nature communications 14:4677 PubMed37542037
2023
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Oncology letters 25:225 PubMed37153045
2023
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British journal of cancer 123:459-470 PubMed32439934
2020
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