AB100613

Human CXCL7 ELISA Kit

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Human CXCL7 ELISA Kit is a Sandwich ELISA for the measurement of Human CXCL7 in Human in Biofluids, Cell Culture Media, Cell/Tissue Extracts samples.

대체 명칭 보기

CTAP3, CXCL7, SCYB7, TGB1, THBGB1, PPBP, Platelet basic protein, PBP, C-X-C motif chemokine 7, Leukocyte-derived growth factor, Macrophage-derived growth factor, Small-inducible cytokine B7, LDGF, MDGF

3 이미지

Sandwich ELISA - Human CXCL7 ELISA Kit (AB100613)

sELISA

Lab

Sandwich ELISA - Human CXCL7 ELISA Kit (AB100613)

CXCL7 measured in biological fluids showing quantity (ng) per mL of tested sample

sELISA

Supplier Data

Sandwich ELISA - Human CXCL7 ELISA Kit (AB100613)

Representative Standard Curve using ab100613.

sELISA

Supplier Data

Sandwich ELISA - Human CXCL7 ELISA Kit (AB100613)

Representative Standard Curve using ab100613.

주요 정보

검출 방식

Colorimetric

샘플 타입

Plasma, Cell culture supernatant, Serum, Cell Lysate

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

< 8.5 pg/mL

검출 범위

4.1 - 1000 pg/mL

분석 플랫폼

Microplate

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Abcam's CXCL7 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CXCL7 in serum, plasma, and cell culture supernatants.

This assay employs an antibody specific for Human CXCL7 coated on a 96-well plate. Standards and samples are pipetted into the wells and CXCL7 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CXCL7 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CXCL7 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Optimization may be required with urine samples.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "84 - 103 %", "average": "= 95.28" }, { "sample": "Serum", "range": "82 - 102 %", "average": "= 92.76" }, { "sample": "Plasma", "range": "83 - 103 %", "average": "= 93.45" } ]

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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100613-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100613-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100613-CMP05", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100613-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"CXCL7 Microplate (12 x 8 wells)", "number":"AB100613-CMP08", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100613-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100613-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human CXCL7", "number":"AB100613-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human CXCL7 Standard (lyophilized)", "number":"AB100613-CMP09", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB100613-CMP03", "productcode":"" } ] } } }

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

-20°C

적절한 장기 보관 조건

-20°C

보관 정보

-20°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The target CXCL7 also known as PBP PBP-172 or PPBP is a chemokine with a molecular mass of around 11 kDa. It is a platelet basic protein (PBP) belonging to the family of CXC chemokines. This protein is extensively expressed in megakaryocytes and stored in the alpha-granules of platelets. Upon platelet activation CXCL7 is released and becomes a potent attractant and activator of neutrophils. It travels through the bloodstream and impacts extracellular signaling processes.

Biological function summary

The chemokine plays a significant role in the regulation of inflammation. CXCL7 is not only an individual entity but can form complexes with glycosaminoglycans on cell surfaces enhancing its functionality. This interaction helps in modulating immune responses by recruiting neutrophils and monocytes to sites of injury or infection. The chemokine contributes to wound healing and tissue repair through its involvement in the inflammatory phase.

Pathways

CXCL7 is involved in the chemokine signaling pathway and is an important mediator in the immune system's response to infections. It is related to other chemokine family proteins like CXCL8 (IL-8) which share similar pathways in inflammatory responses. These proteins interact with specific receptors on leukocytes inducing changes in the cytoskeleton and promoting chemotaxis which is the directed movement towards chemical stimuli.

CXCL7 is associated with various inflammatory conditions and has been implicated in atherosclerosis and rheumatoid arthritis. In these conditions elevated levels of this chemokine correlate with increased recruitment and activation of neutrophils exacerbating inflammation. CXCL7 also shares interactions with other proteins such as CXCL1 affecting the progression and severity of these disorders. The understanding of CXCL7's role in such pathologies offers potential therapeutic avenues for intervention.

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제품 프로토콜

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타겟 정보

LA-PF4 stimulates DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by human synovial cells. NAP-2 is a ligand for CXCR1 and CXCR2, and NAP-2, NAP-2(73), NAP-2(74), NAP-2(1-66), and most potent NAP-2(1-63) are chemoattractants and activators for neutrophils. TC-1 and TC-2 are antibacterial proteins, in vitro released from activated platelet alpha-granules. CTAP-III(1-81) is more potent than CTAP-III desensitize chemokine-induced neutrophil activation.

See full target information PPBP

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제품이 사용된 논문 (5)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cancer & metabolism 13:20 PubMed40312746

2025

Proteomic and lipidomic analysis of low-density lipoprotein identifies potential biomarkers of early estrogen receptor-positive breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yamama Abu Mohsen,Rachel Twitto-Greenberg,Anna Cohen,Gil S Leichner,Lidor Mahler,Hofit Cohen,Yehuda Kamari,Aviv Shaish,Ayelet Harari,Alicia Leikin-Frenkel,Efrat Glick Saar,Tamar Geiger,Sergey Malitsky,Maxim Itkin,Dror Harats,Rom Keshet

Translational stroke research 13:364-369 PubMed34455571

2021

Platelet Activation and Chemokine Release Are Related to Local Neutrophil-Dominant Inflammation During Hyperacute Human Stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander M Kollikowski,Mirko Pham,Alexander G März,Lena Papp,Bernhard Nieswandt,Guido Stoll,Michael K Schuhmann

Cancer epidemiology, biomarkers & prevention : a p 28:1668-1681 PubMed31350263

2019

Potential Markers from Serum-Purified Exosomes for Detecting Oral Squamous Cell Carcinoma Metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Cuiping Li,Yang Zhou,Junjun Liu,Xiaoping Su,Hao Qin,Suhua Huang,Xuanping Huang,Nuo Zhou

Cancer medicine 7:325-335 PubMed29356357

2018

CTAPIII/CXCL7: a novel biomarker for early diagnosis of lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Qiang Du,Encheng Li,Yonge Liu,Wenli Xie,Chun Huang,Jiaqi Song,Wei Zhang,Yijie Zheng,Huiling Wang,Qi Wang

Biomicrofluidics 11:064105 PubMed29204246

2017

Constricted microfluidic devices to study the effects of transient high shear exposure on platelets.

Applications

Unspecified application

Species

Unspecified reactive species

Nesreen Z Alsmadi,Sarah J Shapiro,Christopher S Lewis,Vinit M Sheth,Trevor A Snyder,David W Schmidtke

제품이 사용된 논문 모두 보기

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websiteProtocolBooklet

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human CXCL7 ELISA Kit

Sandwich ELISA - Human CXCL7 ELISA Kit (AB100613)

Sandwich ELISA - Human CXCL7 ELISA Kit (AB100613)

Sandwich ELISA - Human CXCL7 ELISA Kit (AB100613)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

결과 유형

Sensitivity

검출 범위

분석 플랫폼

보완 제품

Assay Kits

Assay Kits

Assay Kits

Assay Kits

Reactivity 정보

제품 세부 정보

Recovery

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (5)

Product promise