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AB326749

Human CYR61 ELISA Kit, Chemiluminescent

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Human CYR61 ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human CYR61 with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 16.46 - 4000 pg/ml with a sensitivity of 5 pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending
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Sandwich ELISA - Human CYR61 ELISA Kit, Chemiluminescent (AB326749)
  • sELISA

Lab

Sandwich ELISA - Human CYR61 ELISA Kit, Chemiluminescent (AB326749)

Dynamic range comparison between the Chemiluminescent Human CYR61 SimpleStep ELISA and colorimetric Human CYR61 SimpleStep ELISA (ab238267) for 1X Cell Extraction Buffer PTR, and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Sandwich ELISA - Human CYR61 ELISA Kit, Chemiluminescent (AB326749)
  • sELISA

Lab

Sandwich ELISA - Human CYR61 ELISA Kit, Chemiluminescent (AB326749)

Standard curve comparison between the Chemiluminescent Human CYR61 SimpleStep ELISA and colorimetric Human CYR61 SimpleStep ELISA (ab238267) in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human CYR61 ELISA Kit, Chemiluminescent (AB326749)
  • sELISA

Lab

Sandwich ELISA - Human CYR61 ELISA Kit, Chemiluminescent (AB326749)

Interpolated concentration of native CYR61 was measured in duplicate at different sample concentrations. Undiluted samples are 100% serum, plasma (citrate), and urine. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions for serum and plasma are made in Sample Diluent 25BP, and sample dilutions for urine and made in Sample Diluent NS.

Sandwich ELISA - Human CYR61 ELISA Kit, Chemiluminescent (AB326749)
  • sELISA

Supplier Data

Sandwich ELISA - Human CYR61 ELISA Kit, Chemiluminescent (AB326749)

Interpolated concentrations of native CYR61 in human HeLa cell extract based on a 250 μg/mL extract load and MDA-MB435S cell extract based on a 500 μg/mL extract load. The concentrations of CYR61 were measured in duplicate and interpolated from the CYR61 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CYR61 concentration was determined to be 1,097 pg/mL in HeLa cell extract and 1,717 pg/mL in MDA-MB-435S cell extract.

Sandwich ELISA - Human CYR61 ELISA Kit, Chemiluminescent (AB326749)
  • sELISA

Supplier Data

Sandwich ELISA - Human CYR61 ELISA Kit, Chemiluminescent (AB326749)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CYR61 concentration was determined to be 170.3 pg/mL with a range of 97. 07 – 254.9 pg/mL.

Sandwich ELISA - Human CYR61 ELISA Kit, Chemiluminescent (AB326749)
  • sELISA

Supplier Data

Sandwich ELISA - Human CYR61 ELISA Kit, Chemiluminescent (AB326749)

Interpolated concentrations of native CYR61 in human breast milk (de-fatted), saliva, and cell culture supernatant (SN) samples.

The concentrations of CYR61 were measured in duplicates, interpolated from the CYR61 standard curves and corrected for sample dilution. Undiluted samples are as follows : milk 100%, saliva 100%, HeLa supernatant 25%, and MDA-MB-453S 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CYR61 concentration was determined to be 1,432 pg/mL in neat milk, 798.2 pg/mL in neat saliva, 8,357 pg/mL neat HeLa cell culture supernatant, and 1,984 pg/mL in neat MDA-MB-453S cell culture supernatant.

주요 정보

검출 방식

Luminescent

샘플 타입

Cell culture supernatant, Serum, Milk, Cell culture extracts, Urine, Saliva, Tissue Extracts, Citrate plasma

Reacts with

Human, Mouse

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 5 pg/mL

검출 범위

16.46 - 4000 pg/mL

분석 소요 시간

55m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Human CYR61 ELISA Kit, Chemiluminescent (ab326749) is a rapid single-wash sandwich 55-min ELISA to measure Human CYR61 in Cell culture supernatant, Serum, Milk, Cell culture extracts, Urine, Saliva, Tissue Extracts, Citrate plasma with a sensitivity of 5 pg/mL.

How the assay works

Human CYR61 ELISA Kit, Chemiluminescent (ab326749) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human CYR61 (ab326749) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-CCN1 antibody [EPR21133-104] - BSA and Aside free (Capture) ab244801
- Anti-CCN1 antibody [EPR21133-20] - BSA and Aside free (Detector) ab245074

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.5" }, { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.8" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "94 - 103 %", "average": "= 103" }, { "sample": "Serum", "range": "108 - 115 %", "average": "= 115" }, { "sample": "Milk", "range": "93 - 96 %", "average": "= 96" }, { "sample": "Cell culture extracts", "range": "80 - 85 %", "average": "= 85" }, { "sample": "Urine", "range": "103 - 113 %", "average": "= 113" }, { "sample": "Saliva", "range": "110 - 119 %", "average": "= 119" }, { "sample": "Tissue Extracts", "range": "80 - 88 %", "average": "= 88" }, { "sample": "Citrate plasma", "range": "86 - 96 %", "average": "= 96" } ]
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제품 구성

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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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제품 프로토콜

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타겟 정보

Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CCN1-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3.
See full target information CCN1

대체 명칭 보기

CYR61, GIG1, IGFBP10, CCN1, CCN family member 1, Cellular communication network factor 1, Cysteine-rich angiogenic inducer 61, Insulin-like growth factor-binding protein 10, Protein CYR61, Protein GIG1, IBP-10, IGF-binding protein 10, IGFBP-10
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websiteProtocolBooklet
en
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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