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AB119589

Human Cystatin C ELISA Kit

Human Cystatin C ELISA 키트

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(6 제품이 사용된 논문 )

Human Cystatin C ELISA Kit is a Sandwich ELISA for the measurement of Human Cystatin C in Human in Cell Culture Media, Biofluids samples.

대체 명칭 보기

Cystatin-C, Cystatin-3, Gamma-trace, Neuroendocrine basic polypeptide, Post-gamma-globulin, CST3

1 이미지
Sandwich ELISA - Human Cystatin C ELISA Kit (AB119589)
  • sELISA

Supplier Data

Sandwich ELISA - Human Cystatin C ELISA Kit (AB119589)

Representative Standard Curve using 119589.

주요 정보

검출 방식

Colorimetric

샘플 타입

Cell culture supernatant, EDTA Plasma, Heparin Plasma, Milk, Serum, Urine

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

< 10 pg/mL

검출 범위

0.312 - 20 ng/mL

분석 플랫폼

Microplate

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보완 제품

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Abcam's Human Cystatin C in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human Cystatin C in cell culture supernatants, serum, plasma (heparin, EDTA) saliva, urine and Human milk.

A Cystatin C specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for Cystatin C is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human Cystatin C amount of sample captured in plate.

Get results in 90 minutes with Human Cystatin C ELISA Kit (ab179883) from our SimpleStep ELISA® range.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 24, "mean": "2.715 ng/mL", "standardDeviation": "0.217", "coefficientOfVariability": "= 8" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 24, "mean": "10.112 ng/mL", "standardDeviation": "0.859", "coefficientOfVariability": "= 8.5" }, { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 16, "mean": "10.374 ng/mL", "standardDeviation": "0.684", "coefficientOfVariability": "= 6.6" }, { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 24, "mean": "0.647 ng/mL", "standardDeviation": "0.051", "coefficientOfVariability": "= 7.9" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 16, "mean": "2.837 ng/mL", "standardDeviation": "0.207", "coefficientOfVariability": "= 7.3" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 16, "mean": "0.687 ng/mL", "standardDeviation": "0.044", "coefficientOfVariability": "= 6.4" } ]
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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab119589-1x96Tests", "assetComponentDetails": [ { "size":"1 x 20 mL", "name":"Wash Buffer (25X)", "number":"AB119589-CMP11", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Stop Solution", "number":"AB119589-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"ABC Diluent Buffer", "number":"AB119589-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"Antibody Diluent Buffer", "number":"AB119589-CMP06", "productcode":"" }, { "size":"4 x 1 Unit", "name":"Plate Seal", "number":"AB119589-CMP01", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Color Developing Agent", "number":"AB119589-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human Cystatin C antibody Microplate (12 x 8 wells)", "number":"AB119589-CMP10", "productcode":"" }, { "size":"1 x 30 mL", "name":"Sample Diluent Buffer", "number":"AB119589-CMP02", "productcode":"" }, { "size":"1 x 100 µL", "name":"Avidin-Biotin-Peroxidase Complex (ABC)", "number":"AB119589-CMP09", "productcode":"" }, { "size":"2 x 20 ng", "name":"Lyophilized recombinant Human Cystatin C standard", "number":"AB119589-CMP08", "productcode":"" }, { "size":"1 x 100 µL", "name":"Biotinylated anti- Human Cystatin C antibody", "number":"AB119589-CMP07", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
-20°C
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제품 프로토콜

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타겟 정보

As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.
See full target information CST3
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제품이 사용된 논문 (6)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Clinical proteomics 21:61 PubMed39487396

2024

Serum proteomics for the identification of biomarkers to flag predilection of COVID19 patients to various organ morbidities.

Applications

Unspecified application

Species

Unspecified reactive species

Madhan Vishal Rajan,Vipra Sharma,Neelam Upadhyay,Ananya Murali,Sabyasachi Bandyopadhyay,Gururao Hariprasad

Antimicrobial agents and chemotherapy 68:e0021924 PubMed39225483

2024

Evaluating nephrotoxicity reduction in a novel polymyxin B formulation: insights from a 3D kidney-on-a-chip model.

Applications

Unspecified application

Species

Unspecified reactive species

Anurag Payasi,Manoj Kumar Yadav,Saransh Chaudhary,Anmol Aggarwal

Asia Pacific allergy 13:158-163 PubMed38094096

2023

Activities and concentration of alpha-1 antitrypsin and cystatin C in serum from patients with house dust mite asthma.

Applications

Unspecified application

Species

Unspecified reactive species

Mailani Dwi Hidayati,Febriana Catur Iswanti,Samsuridjal Djauzi,Sukamto Koesnoe,Mohamad Sadikin

Environment international 177:107997 PubMed37269720

2023

Ambient black carbon reaches the kidneys.

Applications

Unspecified application

Species

Unspecified reactive species

Leen Rasking,Priyanka Koshy,Eva Bongaerts,Hannelore Bové,Marcel Ameloot,Michelle Plusquin,Katrien De Vusser,Tim S Nawrot

Pharmacogenomics and personalized medicine 16:481-489 PubMed37256202

2023

Plasma Homocysteine (Hcy) Concentration Functions as a Predictive Biomarker of SPECT-Evaluated Post-Ischemic Hyperperfusion in Acute Ischemic Stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Yingqiu Wang,Renhua Hou,Yan Liu

Sensors (Basel, Switzerland) 21: PubMed34063596

2021

Development and Evaluation of a Quantitative Fluorescent Lateral Flow Immunoassay for Cystatin-C, a Renal Dysfunction Biomarker.

Applications

Unspecified application

Species

Unspecified reactive species

Satheesh Natarajan,Maria C DeRosa,Malay Ilesh Shah,Joseph Jayaraj
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Product promise

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