Human Cytokeratin 19 ELISA Kit

제품 세부 정보

Human Cytokeratin 19 ELISA Kit (ab252367) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Cytokeratin 19 protein in cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Cytokeratin 19 with 375 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Cytokeratin 19, also known as KRT19, is an intermediate filament protein that gives structure to epithelial cells. Beyond its role as a structural protein, KRT19 is involved in cell signaling pathways including Wnt/Notch and EGR1/PTEN/AKT, although the response to altered expression is highly cell-type dependent. Expression levels of this protein are altered in many types of cancers, and it is currently used as a biomarker for breast cancer.The standard is affinity purified.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.