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AB309390

Human Dectin-1 ELISA Kit

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Human Dectin-1 ELISA Kit is a Sandwich ELISA for the measurement of Human Dectin-1 in Human in Cell Culture Media, Biofluids samples.

대체 명칭 보기

CD369, BGR, CLECSF12, DECTIN1, UNQ539/PRO1082, CLEC7A, C-type lectin domain family 7 member A, Beta-glucan receptor, C-type lectin superfamily member 12, Dendritic cell-associated C-type lectin 1, DC-associated C-type lectin 1, Dectin-1

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Sandwich ELISA - Human Dectin-1 ELISA Kit (AB309390)
  • sELISA

Supplier Data

Sandwich ELISA - Human Dectin-1 ELISA Kit (AB309390)

Example of standard curve using ab309390. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

주요 정보

검출 방식

Colorimetric

샘플 타입

Cell culture supernatant, Plasma, Serum

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 0.082 ng/mL

검출 범위

0.082 - 20 ng/mL

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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보완 제품

Proteins & Peptides

AB276797

Recombinant Mouse Dectin-1 protein (His tag)

SDS-PAGE - Recombinant Mouse Dectin-1 protein (His tag) (AB276797)

  • 0
  • 0
Primary Antibodies

AB311845

Anti-Dectin-1 antibody [EPR28032-27]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Dectin-1 antibody [EPR28032-27] (AB311845)

  • 0
  • 0
Primary Antibodies

AB201337

Anti-CD1a antibody [C1A/711]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1a antibody [C1A/711] (AB201337)

  • 0
  • 0
Primary Antibodies

AB305048

Alexa Fluor® 647 Anti-CD8 alpha antibody [CAL66]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD8 alpha antibody [CAL66] (AB305048)

  • 5
  • 1
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Human Dectin-1 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Dectin-1 in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human Dectin-1 coated on a 96-well plate.
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Recovery

[ { "sample": "Plasma", "range": "93 - 147 %", "average": "= 116.1" }, { "sample": "Serum", "range": "109 - 124 %", "average": "= 116.9" }, { "sample": "Cell culture media", "range": "94 - 140 %", "average": "= 113.3" } ]
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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab309390-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB309390-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"500X HRP-Streptavidin", "number":"AB309390-CMP12", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human Dectin-1 Detection Antibody", "number":"AB309390-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB309390-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human Dectin-1 Protein Standard", "number":"AB309390-CMP11", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B (Item E)", "number":"AB309390-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human Dectin-1 Antibody-coated ELISA Plate", "number":"AB309390-CMP09", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB309390-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D (Item K)", "number":"AB309390-CMP06", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
-20°C
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제품 프로토콜

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타겟 정보

Lectin that functions as a pattern recognizing receptor (PRR) specific for beta-1,3-linked and beta-1,6-linked glucans, which constitute cell wall constituents from pathogenic bacteria and fungi (PubMed : 11567029, PubMed : 12423684). Necessary for the TLR2-mediated inflammatory response and activation of NF-kappa-B : upon beta-glucan binding, recruits SYK via its ITAM motif and promotes a signaling cascade that activates some CARD domain-BCL10-MALT1 (CBM) signalosomes, leading to the activation of NF-kappa-B and MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14) pathways which stimulate expression of genes encoding pro-inflammatory cytokines and chemokines (By similarity). Enhances cytokine production in macrophages and dendritic cells (By similarity). Mediates production of reactive oxygen species in the cell (By similarity). Mediates phagocytosis of C.albicans conidia (PubMed : 17230442). Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation. Stimulates T-cell proliferation. Induces phosphorylation of SCIMP after binding beta-glucans (By similarity).
See full target information CLEC7A
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websiteProtocolBooklet
en
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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