JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB309442

Human Desmocollin 2 ELISA kit

Be the first to review this product! 리뷰 제출

|

(0 출판물)

Human Desmocollin 2 ELISA kit is a Sandwich ELISA for the measurement of Human Desmocollin 2 in Human in Biofluids, Cell Culture Media samples.
1 이미지
Sandwich ELISA - Human Desmocollin 2 ELISA kit (AB309442)
  • sELISA

Supplier Data

Sandwich ELISA - Human Desmocollin 2 ELISA kit (AB309442)

Example of standard curve using ab309442. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

주요 정보

검출 방식

Colorimetric

샘플 타입

Serum, Plasma, Cell culture supernatant

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 21 pg/mL

검출 범위

21 - 5000 pg/mL

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

style
left-column

보완 제품

Primary Antibodies

AB95967

Anti-Desmocollin 2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmocollin 2 antibody (AB95967)

  • 4
  • 2
Assay Kits

AB252360

Human PD-1 ELISA Kit

Recombinant|SimpleStep

ELISA - Human PD-1 ELISA Kit (AB252360)

  • 0
  • 0
Assay Kits

AB277712

Human PD-L1 ELISA Kit [28-8]

Recombinant|SimpleStep

ELISA - Human PD-L1 ELISA Kit [28-8] (AB277712)

  • 0
  • 0
Primary Antibodies

AB272255

PE/Cy5® Anti-Interferon gamma antibody [XMG1.2]

Flow Cytometry (Intracellular) - PE/Cy5® Anti-Interferon gamma antibody [XMG1.2] (AB272255)

  • 0
  • 0
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

제품 세부 정보

Human Desmocollin 2 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Desmocollin 2 in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human desmocollin 2 coated on a 96-well plate.
style
left-column

Precision & recovery 정보

Recovery

[ { "sample": "Serum", "range": "82 - 117 %", "average": "= 99.08" }, { "sample": "Plasma", "range": "83 - 134 %", "average": "= 109.3" }, { "sample": "Cell culture supernatant", "range": "70 - 80 %", "average": "= 73.96" }, { "sample": "Cell culture media", "range": "70 - 80 %", "average": "= 73.96" } ]

style
left-column

제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab309442-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB309442-CMP06", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB309442-CMP09", "productcode":"" }, { "size":"1 x 200 µL", "name":"150X HRP-Streptavidin", "number":"AB309442-CMP12", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human Desmocollin-2 Protein Standard", "number":"AB309442-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB309442-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human Desmocollin-2 Detection Antibody", "number":"AB309442-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB309442-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human Desmocollin-2 Antibody-coated ELISA Plate", "number":"AB309442-CMP01", "productcode":"" } ] } } }
style
left-column

특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
-20°C
style
left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Desmocollin 2 also known as DSC2 is a cadherin protein and an important component of desmosomes which are structures that facilitate cell-cell adhesion in epithelial tissues. It has a molecular weight of approximately 100 kDa. This protein expresses strongly in tissues rich in desmosomes such as the epidermis and cardiac tissue. Desmocollin 2 stabilizes cells against shearing forces ensuring that they adhere tightly by interacting with other desmosomal proteins.
Biological function summary

Desmocollin 2 plays a role in maintaining tissue integrity and cohesion. It participates as a part of the desmosomal complex where it interacts with other cadherin family members like desmogleins and anchors to the intermediate filaments inside the cell. These interactions help to maintain the architecture and structural support during mechanical stress especially in tissues undergoing constant stress like the heart and skin.

Pathways

Desmocollin 2's mechanical role intersects with various cell signaling pathways. Notably it plays a role in the Wnt signaling pathway which is important for cell proliferation and differentiation. The protein also interacts with plakoglobin a protein involved in beta-catenin regulation within these pathways. Desmocollin 2's involvement in cell adhesion and signaling nodes suggests its contribution to further complex signaling cascades.

Desmocollin 2 mutations and disruptions associate with arrhythmogenic right ventricular cardiomyopathy (ARVC) and certain skin blistering conditions. In ARVC the weakened cell adhesion due to desmocollin 2 defects can lead to fibrofatty replacement of myocardium impacting cardiac function. The protein plakoglobin often appears as a related factor in these diseases as both proteins interact at the desmosomal junctions and contribute to cell adhesion integrity.
style
left-column

제품 프로토콜

style
left-column

타겟 정보

A component of desmosome cell-cell junctions which are required for positive regulation of cellular adhesion (PubMed : 33596089). Promotes timely incorporation of DSG2 into desmosome intercellular junctions and promotes interaction of desmosome cell junctions with intermediate filament cytokeratin, via modulation of DSP phosphorylation (PubMed : 33596089). Plays an important role in desmosome-mediated maintenance of intestinal epithelial cell intercellular adhesion strength and barrier function (PubMed : 33596089). Positively regulates wound healing of intestinal mucosa via promotion of epithelial cell migration, and also plays a role in mechanotransduction of force between intestinal epithelial cells and extracellular matrix (PubMed : 31967937). May contribute to epidermal cell positioning (stratification) by mediating differential adhesiveness between cells that express different isoforms. May promote p38MAPK signaling activation that facilitates keratinocyte migration (By similarity).
See full target information DSC2

대체 명칭 보기

CDHF2, DSC3, DSC2, Desmocollin-2, Cadherin family member 2, Desmocollin-3, Desmosomal glycoprotein II, Desmosomal glycoprotein III
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com