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AB314727

Human Desmoglein 2/DSG2 ELISA Kit

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Human Desmoglein 2/DSG2 ELISA Kit is a Sandwich ELISA for the measurement of Human Desmoglein 2/DSG2 in Human in Biofluids, Cell Culture Media samples.

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CDHF5, DSG2, Desmoglein-2, Cadherin family member 5, HDGC

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Sandwich ELISA - Human Desmoglein 2/DSG2 ELISA Kit (AB314727)
  • sELISA

Supplier Data

Sandwich ELISA - Human Desmoglein 2/DSG2 ELISA Kit (AB314727)

Example of standard curve using ab314727. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

주요 정보

검출 방식

Colorimetric

샘플 타입

Serum, Plasma, Cell culture supernatant

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 16 pg/mL

검출 범위

16 - 4000 pg/mL

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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보완 제품

Primary Antibodies

AB150372

Anti-Desmoglein 2/DSG2 antibody [EPR6768]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 2/DSG2 antibody [EPR6768] (AB150372)

  • 4
  • 2
Assay Kits

AB275902

Human Desmoglein 3 ELISA Kit (PVA)

Recombinant|SimpleStep

Sandwich ELISA - Human Desmoglein 3 ELISA Kit (PVA) (AB275902)

  • 0
  • 0
Primary Antibodies

AB95967

Anti-Desmocollin 2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmocollin 2 antibody (AB95967)

  • 4
  • 2
Primary Antibodies

AB209490

Anti-Desmoglein 1/DSG1 antibody [EPR20382]

BOND RX™ Validated|20ul selling size|Recombinant|Advanced Validation|RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 1/DSG1 antibody [EPR20382] (AB209490)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Human Desmoglein 2/DSG2 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Desmoglein 2/DSG2 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human Desmoglein 2/DSG2 coated on a 96-well plate.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human ADAM12, ADAM-8, B7-H3 (CD276), BMPR-IB, Cadherin-13, Cadherin-4, CD155 (PVR), CD229 (SLAMF3), CD48 (SLAMF2), CD58 (LFA-3), CD84 (SLAMF5), CD99, CEACAM-5, Coagulation Factor XIV (Protein C), Cystatin A, Cystatin B, Cystatin E/M, DR3 (TNFRSF25), ErbB4 (HER4), ESAM, FGF-21, Galectin-2, Galectin-9, ICOS, JAM-A (CD321/F11R), JAM-B (VE-JAM/CD322), Kallikrein 5, Midkine, Pentraxin-3 (TSG-14), Pref-1 (DLK-1/FA1), Siglec10, SLAM (CD150), SP-D, Syndecan-4, Testican 2 (SPOCK2), TIM-3 (KIM-3), TLR4, TRAIL (TNFSF10), ULBP-1.

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Recovery

[ { "sample": "Serum", "range": "107 - 143 %", "average": "= 123.8" }, { "sample": "Plasma", "range": "80 - 123 %", "average": "= 108.3" }, { "sample": "Cell culture media", "range": "68 - 90 %", "average": "= 80.08" } ]
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제품 구성

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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
-20°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Desmoglein 2 (DSG2) is a member of the desmoglein family of proteins sometimes referred to as desmoglein-2 or DSG 2. This protein is a calcium-binding transmembrane glycoprotein and part of the cadherin family with a molecular mass of approximately 122 kDa. DSG2 plays a mechanical role in cell-cell adhesion by forming desmosomes which are specialized structures for maintaining the integrity of tissues. DSG2 is mainly expressed in epithelial cells including those found in the heart skin and certain internal organs.
Biological function summary

DSG2 contributes to the structural stability of tissues by locking cells together forming a mechanical and signaling function. The protein is integral to the composition of desmosomes complexes that include other components such as plakoglobin and desmoplakin. These complexes strengthen intercellular adhesion which is essential for tissues that experience constant physical stress like the heart and skin. DSG2 actively participates in cell signaling impacting cellular behavior and communication.

Pathways

DSG2 plays significant roles in the heart development and epidermal differentiation pathways. DSG2 interacts with several other proteins including plakoglobin and desmoplakin to maintain cellular adhesion and tissue morphogenesis. In the cardiac setting the heart development pathway connects DSG2 with other proteins essential for maintaining the integrity and signaling within the cardiac muscle. This interaction helps modulate pathways that ensure proper cardiac function and repair processes.

DSG2 is linked to arrhythmogenic right ventricular cardiomyopathy (ARVC) which affects the heart's rhythm and muscle structure. DSG2 mutations can disrupt desmosomal structure weakening cell adhesion and leading to disease. Another related disorder is pemphigus vulgaris an autoimmune disease where antibodies target DSG2 among other desmogleins disrupting cell adhesion in the skin and mucous membranes. In ARVC DSG2 frequently connects with other proteins like plakoglobin that are involved in desmosomal interactions leading to the structural and functional deficits seen in the disease.
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제품 프로토콜

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타겟 정보

A component of desmosome cell-cell junctions which are required for positive regulation of cellular adhesion (PubMed : 17559062, PubMed : 38395410). Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. Required for proliferation and viability of embryonic stem cells in the blastocyst, thereby crucial for progression of post-implantation embryonic development (By similarity). Maintains pluripotency by regulating epithelial to mesenchymal transition/mesenchymal to epithelial transition (EMT/MET) via interacting with and sequestering CTNNB1 to sites of cell-cell contact, thereby reducing translocation of CTNNB1 to the nucleus and subsequent transcription of CTNNB1/TCF-target genes (PubMed : 29910125). Promotes pluripotency and the multi-lineage differentiation potential of hematopoietic stem cells (PubMed : 27338829). Plays a role in endothelial cell sprouting and elongation via mediating the junctional-association of cortical actin fibers and CDH5 (PubMed : 27338829). Promotes cardiomyocyte cell homeostasis and desmosome junction formation at intercalated disks, as a result plays a role in the maintenance of cardiac conduction and heart chamber integrity (By similarity). Positively regulates pancreatic islet development and maintenance of endothelial cell barrier integrity in the pancreas, therefore involved in the controlled release of insulin from islet cells into the circulation in response to glucose (By similarity). Plays a role in limiting inflammatory infiltration and the apoptotic response to injury in kidney tubular epithelial cells, potentially via its role in maintaining cell-cell adhesion and the epithelial barrier (PubMed : 38395410). Acts as a positive modulator of CSK and EGFR activation via sequestering them away from lipid rafts, this is independent of its role in desmosome cell junctions (PubMed : 26918609). Also disrupts the localization of CAV1 to lipid rafts resulting in its distribution throughout the cytoplasm (PubMed : 26918609).
See full target information DSG2
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