Human DKK3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human DKK3 with a sensitivity of 19.3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
대체 명칭 보기
REIC, UNQ258/PRO295, DKK3, Dickkopf-related protein 3, Dickkopf-3, Dkk-3, hDkk-3
- sELISA
Lab
Sandwich ELISA - Human DKK3 ELISA Kit (AB236710)
Example of human DKK3 standard curve in Sample Diluent NS prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Lab
Sandwich ELISA - Human DKK3 ELISA Kit (AB236710)
Interpolated concentrations of native DKK3 in human cell culture supernatant samples. The concentrations of DKK3 were measured in duplicates, interpolated from the DKK3 standard curves and corrected for sample dilution. Undiluted samples are as follows : HUVEC supernatant 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean DKK3 concentration was determined to be 19.0 ng/mL in HUVEC supernatant.
- sELISA
Lab
Sandwich ELISA - Human DKK3 ELISA Kit (AB236710)
Interpolated concentrations of native DKK3 in human serum and plasma samples. The concentrations of DKK3 were measured in duplicates, interpolated from the DKK3 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 10%, plasma (citrate) 10%, plasma (heparin) 10%, and plasma (EDTA) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean DKK3 concentration was determined to be 37.5 ng/mL in serum, 30.5 ng/mL in plasma (citrate), 29.6 ng/ml in plasma (heparin), and 29.0 ng/mL in plasma (EDTA).
- sELISA
Supplier Data
Sandwich ELISA - Human DKK3 ELISA Kit (AB236710)
Serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean DKK3 concentration was determined to be 40.4 ng/mL with a range of 27.3 – 65.8 ng/mL.
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제품 세부 정보
Human DKK3 ELISA Kit (ab236710) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of DKK3 protein in cit plasma, edta plasma, hep plasma, serum, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human DKK3 with 34.1 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Precision
Recovery
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Clinics (Sao Paulo, Brazil) 79:100360 PubMed38678874
2024
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